Gremlin在增殖性糖尿病视网膜病变患者玻璃体腔中的表达
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摘要
目的探讨增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)患者玻璃体中gremlin的水平与PDR的关系。方法收集26例PDR患者的玻璃体为试验组,同时收集20例特发性黄斑前膜患者的玻璃体为对照组,采用ELISA方法测定玻璃体中gremlin的水平。结果试验组患者玻璃体中gremlin水平为[(67.786±33.963)ng/ml)],明显高于对照组[(42.265±7.626)ng/ml)],对比差异有统计学意义(P<0.001)。结论 Gremlin在PDR患者玻璃体中的高表达可能对PDR的发生发展起着重要的作用。
Objective To investigate the levels of gremlin in the vitreous from patients with proliferative diabetic retinopathy(PDR).Methods The vitreous from 26 patients with PDR were collected as the experimental group, while the vitreous from 20 patients with idiopathic anterior macular membrane were collected as the control group. The level of gremlin in the vitreous was determined by ELISA.Results The level of gremlin in the vitreous of the experimental group [(67.786±33.963)ng/ml)] was significantly higher than that of the control group [(42.265±7.626)ng/ml)](P<0.001).Conclusion The high expression of gremlin in the vitreous of PDR patients may play an important role in the occurrence and development of PDR.
Objective To investigate the levels of gremlin in the vitreous from patients with proliferative diabetic retinopathy(PDR).Methods The vitreous from 26 patients with PDR were collected as the experimental group, while the vitreous from 20 patients with idiopathic anterior macular membrane were collected as the control group. The level of gremlin in the vitreous was determined by ELISA.Results The level of gremlin in the vitreous of the experimental group [(67.786±33.963)ng/ml)] was significantly higher than that of the control group [(42.265±7.626)ng/ml)](P<0.001).Conclusion The high expression of gremlin in the vitreous of PDR patients may play an important role in the occurrence and development of PDR.
引文
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[12] Topol LZ,Marx M,Laugier D,et al.Identification of drm,a novel gene whose expression is suppressed in transformed cells and which can inhibit growth of normal but not transformed cells in culture [J].Mol Cell Biol,1997,17(8):4801-4810.
[13] Wang SN,Lapage J,Hirschberg R.Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy [J].J Am Soc Nephrol,2001,12:2392 -2399.
[14] Wada J,Makino H,Kanwar YS.Gene expression and identification of gene therapy targets in diabetic nephropathy [J].Kidney Int,2002,61(1 Suppl):S73-s78.
[15] Lappin DW,McMahon R,Murphy M,et al.Gremlin:an example of the re-emergence of developmental programmes in diabetic nephropathy[J].Nephrol Dial Transplant,2002,17 Suppl 9:65-67.
[16] Huang H,Huang H,Li Y,et al.Gremlin induces cell proliferation and extra cellular matrix accumulation in mouse mesangial cells exposed to high glucose via the ERK1/2 pathway [J].BMC Nephrol,2013,14:33.
[17] Boers W,Aarrass S,Linthorst C,et al.Transcriptional profiling reveals novel markers of liver fibrogenesis:gremlin and insulin-like growth factor-binding proteins [J].J Biol Chem,2006,281(24):16289-16295.
[18] Kane R,Stevenson L,Godson C,et al.Gremlin gene expression in bovine retinal pericytes exposed to elevated glucose [J].Br J Ophthalmol,2005,89(12):1638-1642.
[2] Mitola S,Ravelli C,Moroni E,et al.Gremlin is a novel agonist of the major proangiogenic receptor VEGFR2 [J].Blood,2010,116(18):3677-3680.
[3] Kaur C,Foulds WS,Ling EA.Blood-retinal barrier in hypoxic ischaemic conditions:basic concepts,clinical features and management [J].Prog Retin Eye Res,2008,27:622-647.
[4] Hall AP.Review of the pericyte during angiogenesis and its role in cancer and diabetic retinopathy [J].Toxicol Pathol,2006,34:763-775.
[5] Camera A,Hopps E,Caimi G.Diabetic microangiopathy:physiopathological,clinical and therapeutic aspects [J].Minerva Endocrinol,2007,32:209-229.
[6] Roy S,Sato T,Paryani G,et al.Downregulation of fibronectin overexpression reduces basement membrane thickening and vascular lesions in retinas of galactose-fed rats [J].Diabetes,2003,52:1229-1234.
[7] Roy S,Sala R,Cagliero E,et al.Overexpression of fibronectin induced by diabetes or high glucose:phenomenon with a memory [J].Proc Natl Acad Sci USA,1990,87:404-408.
[8] Spirin KS,Saghizadeh M,Lewin SL,et al.Basement membrane and growth factor gene expression in normal and diabetic human retinas [J].Curr Eye Res,1990,18:490-499.
[9] Ljubimov AV,Burgeson RE,Butkowski RJ,et al.Basement membrane abnormalities in human eyes with diabetic retinopathy [J].J Histochem Cytochem,1996,44:1469-1479.
[10] Snead DR,James S,Snead MP.Pathological changes in the vitreoretinal junction 1:epiretinal membrane formation [J].Eye,2008,22:1310-1317.
[11] Funatsu H,Yamashita H,Noma H.Risk evaluation of outcome of vitreous surgery for proliferative diabetic retinopathy based on vitreous level of vascular endothelial growth factor and angiotensin Ⅱ[J].Br J Ophthalmol,2004,88(8):1064-1068.
[12] Topol LZ,Marx M,Laugier D,et al.Identification of drm,a novel gene whose expression is suppressed in transformed cells and which can inhibit growth of normal but not transformed cells in culture [J].Mol Cell Biol,1997,17(8):4801-4810.
[13] Wang SN,Lapage J,Hirschberg R.Loss of tubular bone morphogenetic protein-7 in diabetic nephropathy [J].J Am Soc Nephrol,2001,12:2392 -2399.
[14] Wada J,Makino H,Kanwar YS.Gene expression and identification of gene therapy targets in diabetic nephropathy [J].Kidney Int,2002,61(1 Suppl):S73-s78.
[15] Lappin DW,McMahon R,Murphy M,et al.Gremlin:an example of the re-emergence of developmental programmes in diabetic nephropathy[J].Nephrol Dial Transplant,2002,17 Suppl 9:65-67.
[16] Huang H,Huang H,Li Y,et al.Gremlin induces cell proliferation and extra cellular matrix accumulation in mouse mesangial cells exposed to high glucose via the ERK1/2 pathway [J].BMC Nephrol,2013,14:33.
[17] Boers W,Aarrass S,Linthorst C,et al.Transcriptional profiling reveals novel markers of liver fibrogenesis:gremlin and insulin-like growth factor-binding proteins [J].J Biol Chem,2006,281(24):16289-16295.
[18] Kane R,Stevenson L,Godson C,et al.Gremlin gene expression in bovine retinal pericytes exposed to elevated glucose [J].Br J Ophthalmol,2005,89(12):1638-1642.