摘要
目的探索~(99)Tc~m-MIBI脂质体纳米粒的制备方法,考察其在体外条件下的物理、生物学表征和稳定性,并研究其在小鼠体内的生物学分布特征。方法采用乙醇滴注-超声分散工艺制备~(99)Tc~m-MIBI脂质体纳米粒。测定其粒径及包封率指标。在体外37℃条件下,观察~(99)Tc~m-MIBI脂质体纳米粒在正常人血清和生理盐水中(NS)不同时间点的放化纯及其稳定性,研究其在小鼠体内15、60、120 min的分布特征。结果乙醇滴注-超声分散方法制备的~(99)Tc~m-MIBI脂质体纳米粒在电镜下观察呈球形、均匀,平均粒径(168.2±18.6)nm。体外稳定性实验表明,在正常人血清和NS中将~(99)Tc~m-MIBI脂质体纳米粒孵育15、30、60、120 min,其放化纯分别达96%、93%、90%、89%和92%、89%、86%、85%。体内生物分布实验表明,与~(99)Tc~m-MIBI比较,静脉注射~(99)Tc~m-MIBI脂质体纳米粒后,在观察时间内发现脾脏摄取显著,肾脏的放射性摄取率较低。结论~(99)Tc~m-MIBI脂质体纳米粒的制备方法简单,具有较为理想的物理、生物学表征,在血清中的稳定性较好。与~(99)Tc~m-MIBI比较肾脏摄取率低,在动物体内的循环时间延长。
Objective To explore the method of preparing ~(99)Tc~m-MIBIliposome nanoparticles,investigate its' physical,biological characterization and stability in vitro,and study its biological distribution characteristics in mice. Methods The ~(99)Tc~m-MIBIliposome nanoparticles were prepared by the ethanol infusion-ultrasonic dispersion process. The particle size and encapsulation efficiency were measured. The radioactivity purity and stability of ~(99)Tc~m-MIBIliposome nanoparticles in normal human serum and normal saline( NS) at different time points were observed under the condition of 37 ℃ in vitro. The distribution characteristics of 15,60,and 120 min in mice were studied. Results The ~(99)Tc~m-MIBIliposome nanoparticles prepared by ethanol infusion-ultrasonic dispersion method were spherical and homogeneous under the electron microscope,with an average diameter of( 168. 2 ± 18. 6) nm. In vitro stability test showed that ~(99)Tc~m-MIBIliposome nanoparticles incubated 15,30,60,120 min in normal serum and NS respectively,and their radiochemical purity was96%,93%,90%,89% and 92%,89%,86% and 85%,respectively. In vivo biodistribution experiment showed that compared with ~(99)Tc~m-MIBI,after intravenous injection of ~(99)Tc~m-MIBIliposome nanoparticles,the spleen uptake was significantly observed and the radioactivity uptake rate of kidney was low during the observation time. Conclusion The preparation method of ~(99)Tc~m-MIBIliposome nanoparticles is simple and has ideal physical,biological characterization,and the stability in serum is good. Compared with ~(99)Tc~m-MIBIthe kidney uptake rate was low,and the circulation time in vivo of the mice was prolonged.
引文
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