纳米场效应晶体管生物传感器在肿瘤早期检测中的应用
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摘要
肿瘤的早期检测对于肿瘤的早期发现、诊断及治疗具有重要意义。基于硅纳米线、石墨烯、二硫化钼的纳米场效应晶体管生物传感器,由于灵敏度高和特异性好、分析速度快、免标记、廉价、能够微型化和一体化等优点,能够检测肿瘤相关标志物微RNA和蛋白质等,在肿瘤的早期检测中可以发挥重要作用。
The timely detection of tumors is of great significance for the early detection, diagnosis and treatment for cancer. Nano-field effect transistor biosensors, based on silicon nanowires, graphene and molybdenum disulfide, can detect tumor derived miRNA and protein markers due to the several critical advantages including high sensitivity and selectivity, rapid analysis, label-free detection, easy operation and so on, which can play an important role in the early detection of tumor.
The timely detection of tumors is of great significance for the early detection, diagnosis and treatment for cancer. Nano-field effect transistor biosensors, based on silicon nanowires, graphene and molybdenum disulfide, can detect tumor derived miRNA and protein markers due to the several critical advantages including high sensitivity and selectivity, rapid analysis, label-free detection, easy operation and so on, which can play an important role in the early detection of tumor.
引文
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[4] Liu C, Yan X, Zhang X, et al. Evaluation of x-ray diffraction enhanced imaging in the diagnosis of breast cancer[J]. Phys Med Biol, 2007,52(2):419-427.
[5] Kong RM, Ding L, Wang Z, et al. A novel aptamerfunctionalized MoS2 nanosheet fluorescent biosensor for sensitive detection of prostate specific antigen[J].Anal Bioanal Chem, 2015,407(2):369-377.
[6] Wang H, Wang X, Wang J, et al. A SPR biosensor based on signal amplification using antibody-QD conjugates for quantitative determination of multiple tumor markers[J]. Sci Rep, 2016,6:33 140.
[7] Jang HS, Park KN, Chang DK, et al. Optical fiber SPR biosensor with sandwich assay for the detection of prostate specific antigen[J]. Opt Commun, 2009, 282(14):2 827-2 830.
[8] Zheng C, Huang L, Zhang H, et al. Fabrication of ultrasensitive field-effect transistor DNA biosensors by a directional transfer technique based on CVD-grown Graphene[J]. Acs Appl Mater Interfaces, 2015, 7(31):16 953-16 959.
[9] Zhang AQ, Lieber CM. Nano-bioelectronics[J]. Chem Rev, 2016,116(1):215-257.
[10] Lin P, Yan F. Organic thin-film transistors for chemical and biological sensing[J]. Adv Mater, 2012, 24(1):34-51.
[11] Huang Y, Dong X, Liu Y, et al. Graphene-based biosensors for detection of bacteria and their metabolic activities[J]. J Mater Chem, 2011,21(33):12 358-12 362.
[12] Lin CT, Loan PTK, Chen TY, et al. Label-free electrical detection of DNA hybridization on graphene using hall effect measurements:Revisiting the sensing mechanism[J]. Adv Funct Mater, 2013,23(18):2 301-2 307.
[13] Wang L, Wang Y, Wong JI, et al. Functionalized MoS2nanosheet-based field-effect biosensor for labelfree sensitive detection of cancer marker proteins in solution[J]. Small, 2014,10(6):1 101-1 105.
[14] Zhang GJ, Chua JH, Chee RE, et al. Label-free direct detection of miRNA with silicon nanowire biosensors[J]. Biosens Bioelectron, 2009,24(8):2 504-2 508.
[15] Stine R, Mulvaney SP, Robinson JT, et al. Fabrication, optimization, and use of graphene field effect sensors[J]. Anal Chem, 2013,85(2):509-521.
[16] Gao A, Lu N, Dai P, et al. Label-free and ultrasensitive detection of microRNA biomarkers in lung cancer cells based on silicon nanowire FET biosensors:Proceedings of the 17th International Conference on SolidState Sensors, Actuators and Microsystems(Transducers&Eurosensors XXVII)[C]. Barcelona, Spain:IEEE, 2013:2 439-2 442.
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[19] Zheng G, Patolsky F, Cui Y, et al. Multiplexed electrical detection of cancer markers with nanowire sensor arrays[J]. Nat Biotechnol, 2005,23(10):1 294-1 301.
[20] Huang YW, Wu CS, Chuang CK, et al. Real-time and label-free detection of the prostate-specific antigen in human serum by a polycrystalline silicon nanowire field-effect transistor biosensor[J]. Anal Chem, 2013,85(16):7 912-7 918.
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[23] Stern E, Vacic A, Rajan NK, et al. Label-free biomarker detection from whole blood[J]. Nat Nanotechnol, 2010,5(2):138-142.
[24] Chen HC, Chen YT, Tsai RY, et al. A sensitive and selective magnetic graphene composite-modified polycrystalline-silicon nanowire field-effect transistor for bladder cancer diagnosis[J]. Biosens Bioelectron, 2015,66:198-207.
[25] Spanjaard RA, Whren KM, Graves C, et al. Tumor necrosis factor receptor superfamily member troy is a novel melanoma biomarker and potential therapeutic target[J]. Int J Cancer, 2007,120(6):1 304-1 310.
[26] Maedler C, Kim D, Spanjaard RA, et al. Sensing of the melanoma biomarker troy using silicon nanowire field-effect transistors[J]. ACS Sensors, 2016,1(6):696-701.
[27] Tran DP, Wolfrum B, Stockmann R, et al. CMOScompatible silicon nanowires on-a-Chip:Fabrication and Pre-clinical validation for the detection of a cancer prognostic protein marker in serum[J]. Anal Chem, 2014,87(3):1 662-1 668.
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[29] Cai B, Huang L, Zhang H, et al. Gold nanoparticlesdecorated graphene field-effect transistor biosensor for femtomolar microRNA detection[J]. Biosens Bioelectron, 2015,74:329-334.
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[32] Rajesh D, Gao Z, Vishnubhotla R, et al. Genetically engineered antibody functionalized platinum nanoparticles modified CVD-graphene nanohybrid transistor for the detection of breast cancer biomarker, her3[J]. Adv Mater Interfaces, 2016,3(17):1600124.
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[35] Kwon OS, Park SJ, Hong JY, et al. Flexible FETtype VEGF aptasensor based on nitrogen-doped graphene converted from conducting polymer[J]. ACS Nano, 2012,6(2):1 486-1 493.
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[41] Nam H, Oh BR, Chen P, et al. Multiple MoS2transistors for sensing molecule interaction kinetics[J]. Sci Rep, 2015,5:10 546.
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