无偿献血者意外抗体检测情况调查
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摘要
目的:回顾性分析近3年无偿献血者意外抗体检出频率及特异性,探讨对献血者进行意外抗体筛查的意义,为临床输血前检查提供依据。方法:应用微板法和试管法对196 311例无偿献血者血液中的意外抗体进行筛查和确认。结果:196 311例无偿献血者血液中检出意外抗体296例,检出率0.15%。1 170例RhD阴性献血者中女性意外抗体的检出率(2.14%)明显高于男性献血者(0.28%)(P<0.05)。检出意外抗体312例(包括15例重复献血者共检出31例次),其中ABO血型系统12例,Rh血型系统10例,MNS血型系统84例,P血型系统110例,Lewis血型系统10例,未鉴定出特异性86例。结论:为保障患者安全用血,有必要对献血者血液进行意外抗体筛查,但需要对筛查方法进行验证、遴选,使用适宜的筛查方法筛查出更多有临床意义的抗体。
Objective:A retrospective study has been peformed to analyze the frequency and specificity of unexpected erythrocyte antibodies among blood donors in our center in past three years,to explore the significance of unexpected antibody screening for blood donors,and to provide a basis for pre-transfusion examination.Method:The micro-plate method and tube test were used to screen and identify unexpected antibodies in 196 311 blood donors.Result:A total of 296 unexpected antibodies were detected in 196 311 blood donors.The detection rate was 0.15%.The detection rate of unexpected antibodies in females(2.14%)was significantly higher than that in males(0.28%)in 1170 RhD-negative blood donors(P<0.05).312 cases of unexpected antibodies were detected(including31 cases of 15 repeated blood donors),including 12 cases of ABO blood group system,10 cases of Rh blood type system,84 cases of MNS blood type system,110 cases of P blood type system,and 10 cases of Lewis blood type system.and 86 cases of nonspecific antibodies.Conclusion:In order to ensure the safety of clinical blood transfusion,it is necessary to screen the unexpected antibodies in blood donors.The selection of screening methods need further verification,in order to detect more clinically significant antibody.
Objective:A retrospective study has been peformed to analyze the frequency and specificity of unexpected erythrocyte antibodies among blood donors in our center in past three years,to explore the significance of unexpected antibody screening for blood donors,and to provide a basis for pre-transfusion examination.Method:The micro-plate method and tube test were used to screen and identify unexpected antibodies in 196 311 blood donors.Result:A total of 296 unexpected antibodies were detected in 196 311 blood donors.The detection rate was 0.15%.The detection rate of unexpected antibodies in females(2.14%)was significantly higher than that in males(0.28%)in 1170 RhD-negative blood donors(P<0.05).312 cases of unexpected antibodies were detected(including31 cases of 15 repeated blood donors),including 12 cases of ABO blood group system,10 cases of Rh blood type system,84 cases of MNS blood type system,110 cases of P blood type system,and 10 cases of Lewis blood type system.and 86 cases of nonspecific antibodies.Conclusion:In order to ensure the safety of clinical blood transfusion,it is necessary to screen the unexpected antibodies in blood donors.The selection of screening methods need further verification,in order to detect more clinically significant antibody.
引文
[1]李静.安阳无偿献血者与临床患者不规则抗体调查分析[J].河南医学研究,2015,24(9):121-122.
[2]徐向华.22682例无偿献血者血液标本的不规则抗体筛查结果[J].中国输血杂志,2015,28(9):1136-1137.
[3]戎霞,张雪梅,黄珂.广州地区无偿献血者不规则抗体筛查结果分析[J].热带医学杂志,2014,14(2):176-178,183.
[4]张慧贤,赵倩,王艳彬,等.石家庄地区献血者Rh阴性血清学表型及不规则抗体检出情况[J].临床血液学杂志,2017,30(10):800-801.
[5] Takeshita A,Watanabe H,Fi jihara H.Collaborative study of irregular erythrocyte antibodies in Japan:results from the Japanesestudy group of allo-immunity and antigen diversity in Asian populations[J].Transfus Apher Sci,2010,43:3-8.
[6]燕备战,孔存权,朱伟彥,等.不规则抗体中抗-M抗体的检测及意义[J].临床血液学杂志,2016,29(2):99-102.
[7] Philip J,Kushwaha N,Jain N.Report of two cases of anti-M antibody in antenatal patients[J].Asian J Transfus Sci,2015,9:89-91.
[8] Daniels G.Human Blood groups[M].3rded.Chirhester:John Wiley&Sons,2013:163-164.
[9]黄雅,林梦霞,吴月清,等.抗-P1导致ABO血型正反定型不符1例[J].临床血液学杂志,2018,31(4):323-325.
[10]张璐,袁红,伍钢.抗M抗体、抗Lea抗体以及自身冷抗体引起的输血交叉配血不合1例[J].临床血液学杂志,2017,30(4):325-326.
[2]徐向华.22682例无偿献血者血液标本的不规则抗体筛查结果[J].中国输血杂志,2015,28(9):1136-1137.
[3]戎霞,张雪梅,黄珂.广州地区无偿献血者不规则抗体筛查结果分析[J].热带医学杂志,2014,14(2):176-178,183.
[4]张慧贤,赵倩,王艳彬,等.石家庄地区献血者Rh阴性血清学表型及不规则抗体检出情况[J].临床血液学杂志,2017,30(10):800-801.
[5] Takeshita A,Watanabe H,Fi jihara H.Collaborative study of irregular erythrocyte antibodies in Japan:results from the Japanesestudy group of allo-immunity and antigen diversity in Asian populations[J].Transfus Apher Sci,2010,43:3-8.
[6]燕备战,孔存权,朱伟彥,等.不规则抗体中抗-M抗体的检测及意义[J].临床血液学杂志,2016,29(2):99-102.
[7] Philip J,Kushwaha N,Jain N.Report of two cases of anti-M antibody in antenatal patients[J].Asian J Transfus Sci,2015,9:89-91.
[8] Daniels G.Human Blood groups[M].3rded.Chirhester:John Wiley&Sons,2013:163-164.
[9]黄雅,林梦霞,吴月清,等.抗-P1导致ABO血型正反定型不符1例[J].临床血液学杂志,2018,31(4):323-325.
[10]张璐,袁红,伍钢.抗M抗体、抗Lea抗体以及自身冷抗体引起的输血交叉配血不合1例[J].临床血液学杂志,2017,30(4):325-326.