马缨杜鹃Actin基因片段的克隆及序列分析
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摘要
为研究马缨杜鹃相关功能基因的表达模式提供内参基因,本研究根据Gen Bank中公布的锦绣杜鹃肌动蛋白基因(Actin)的保守序列设计引物,以马缨杜鹃c DNA为模板,利用RT-PCR方法克隆得到马缨杜鹃Actin基因部分c DNA片段,将其命名为Rd Actin。结果显示:该基因片段长度为468 bp,编码156个氨基酸;保守域分析表明该基因具有Actin的功能保守域,同时Rd Actin与其它植物Actin的氨基酸序列同源性达97%以上。
In order to provide internal reference gene for the study of functional genes expression patterns of Rhododendron delavayi,we designed the primer on the basis of Aticn from Rhododendron x pulchrum in the Genebank. Using the c DNA from Rhododendron delavayi as template,we obtained Actin gene fragment by RT-PCR method,and named it as Rd Actin. The results showed that this Actin gene fragment was 468 bp,encoding a protein of 156 amino acids. Meanwhile,Rd Actin had the functional domains of Actin,and shared over 97% amino acid sequence homology with other plants actins.
In order to provide internal reference gene for the study of functional genes expression patterns of Rhododendron delavayi,we designed the primer on the basis of Aticn from Rhododendron x pulchrum in the Genebank. Using the c DNA from Rhododendron delavayi as template,we obtained Actin gene fragment by RT-PCR method,and named it as Rd Actin. The results showed that this Actin gene fragment was 468 bp,encoding a protein of 156 amino acids. Meanwhile,Rd Actin had the functional domains of Actin,and shared over 97% amino acid sequence homology with other plants actins.
引文
[1]李苇洁,陈训.马缨杜鹃林区枯落物与土壤持水量特性研究[J].贵州科学,2005,23(2):60-65.
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[4]VIDALI L,HEPLER P K.Actin and pollen tube growth[J].Protoplasma,2001,215:64-76.
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[6]陈颖,王刚,赵俊霞.高等植物体内的肌动蛋白[J].生物学通报,2003,38(1):13-15.
[7]张少斌,刘国琴.植物肌动蛋白异型体研究进展[J].植物学通报,2006,23(3):242-248.
[8]田云芳,刘艺平,李涵,等.蕙兰Actin基因的克隆及序列分析[J].河南农业科学,2013,42(6):107-111.
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[11]王生银,张永超,李莉,等.拒盐型盐生植物小花碱茅肌动蛋白基因片段的克隆及序列分析[J].基因组学与应用生物学,2009,4(28):673-677.
[12]许娟,罗兴录.木薯Actin基因片段的克隆及序列分析[J].生物技术通报,2011(6):65-70.
[13]袁秀云,田云芳,蒋素华,等.Actin基因片段的克隆及序列分析[J].中国农学通报,2012,28(13):243-248.
[14]THOMAS C,MEYER D,WOLFF M,et al.Molecular characterization and spatial expression of the sunflower ABP1gene[J].Plant molecular biology,2003,52:1025-1036.
[15]袁秀云,田云芳,蒋素华,等.Actin基因片段的克隆及序列分析[J].中国农学通报,2012,28(13):243-248.
[16]AKIRA N,DAIKI M,YUTAKA K,et al.Isolation and expression analysis of flavonoid biosynthesis genes in evergreen azalea[J].Scientia Horticulturae,2008,118:314-320.
[17]美国国立生物技术信息中心.NCBI数据库[EB/OL].[2018-05-22].http://www.ncbi.nlm.nih.gov/.
[18]瑞士生物信息学研究所.蛋白质分析系统[EB/OL].[2018-05-22].https://www.expasy.org/.
[19]美国国立生物技术信息中心.NCBI数据库[EB/OL].[2018-05-22].nlm.nih.gov/Structure/cdd/wrpsb.cgi?RID=GWMUXPNA014&mode=all.
[20]郭艳,张美,夏快飞,等.厚藤Actin基因片段的克隆及序列分析[J].氨基酸和生物资源,2016,38(3):59-62.
[21]陈颖,王刚,赵俊霞.高等植物体内的肌动蛋白[J].生物学通报,2003,38(1):13-15.
[2]章绍尧,丁炳扬.浙江植物志:总论卷[M].杭州:浙江科学技术出版社,1993.
[3]阮海星,王子坚,吴克枫,等.黔西北马缨杜鹃花红色素的毒性试验[J].植物资源与环境,1997,6(1):25-28.
[4]VIDALI L,HEPLER P K.Actin and pollen tube growth[J].Protoplasma,2001,215:64-76.
[5]COLLINGS D A,HARPER J D I,MARC J,et al.Life in the fast lane:Actinbased motility of plant peroxisomes[J].Canadian journal of botany-revue canadienne de botanique,2002,80(4):430-441.
[6]陈颖,王刚,赵俊霞.高等植物体内的肌动蛋白[J].生物学通报,2003,38(1):13-15.
[7]张少斌,刘国琴.植物肌动蛋白异型体研究进展[J].植物学通报,2006,23(3):242-248.
[8]田云芳,刘艺平,李涵,等.蕙兰Actin基因的克隆及序列分析[J].河南农业科学,2013,42(6):107-111.
[9]张磊,刘志鹏,张吉宇,等.箭筈豌豆两个肌动蛋白基因片段的克隆及序列分析[J].生物技术通报,2010(9):70-75.
[10]周海飞,赵武玲,阎隆飞.玉兰肌动蛋白基因的克隆与序列分析[J].农业生物技术学报,2001,9(3):274-278.
[11]王生银,张永超,李莉,等.拒盐型盐生植物小花碱茅肌动蛋白基因片段的克隆及序列分析[J].基因组学与应用生物学,2009,4(28):673-677.
[12]许娟,罗兴录.木薯Actin基因片段的克隆及序列分析[J].生物技术通报,2011(6):65-70.
[13]袁秀云,田云芳,蒋素华,等.Actin基因片段的克隆及序列分析[J].中国农学通报,2012,28(13):243-248.
[14]THOMAS C,MEYER D,WOLFF M,et al.Molecular characterization and spatial expression of the sunflower ABP1gene[J].Plant molecular biology,2003,52:1025-1036.
[15]袁秀云,田云芳,蒋素华,等.Actin基因片段的克隆及序列分析[J].中国农学通报,2012,28(13):243-248.
[16]AKIRA N,DAIKI M,YUTAKA K,et al.Isolation and expression analysis of flavonoid biosynthesis genes in evergreen azalea[J].Scientia Horticulturae,2008,118:314-320.
[17]美国国立生物技术信息中心.NCBI数据库[EB/OL].[2018-05-22].http://www.ncbi.nlm.nih.gov/.
[18]瑞士生物信息学研究所.蛋白质分析系统[EB/OL].[2018-05-22].https://www.expasy.org/.
[19]美国国立生物技术信息中心.NCBI数据库[EB/OL].[2018-05-22].nlm.nih.gov/Structure/cdd/wrpsb.cgi?RID=GWMUXPNA014&mode=all.
[20]郭艳,张美,夏快飞,等.厚藤Actin基因片段的克隆及序列分析[J].氨基酸和生物资源,2016,38(3):59-62.
[21]陈颖,王刚,赵俊霞.高等植物体内的肌动蛋白[J].生物学通报,2003,38(1):13-15.