硬核资源遗传多样性的AFLP分析（英文）
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摘要
为了解云南省硬核[Scleropyrum wallichianum (Wight et Arn.) Arn.]的遗传多样性,采用AFLP标记分析了7个居群84份种质材料的遗传变异。结果表明,从64对引物组合中挑选出多态性较好的引物8对,共扩增出1 728条带,其中多态性条带1 388条,多态性百分率为80.14%。硬核在物种水平的多样性指数分别为Na=1.416, Ne=1.179, H=0.137, I=0.225,在居群水平上分别为H=0.111,I=0.175;在遗传相似性系数为0.52时,这些种质材料可分为3组,其中易武居群具有丰富的遗传变异,大部分的遗传变异存在于居群内,而在0.05置信区间内居群间遗传变异仅为11.5%;居群间的遗传距离和地理距离无显著相关性(r=0.0323, P=0.5820)。因此,硬核资源可采用就地和迁地保护策略,以增加其遗传多样性。
In order to understand the genetic diversity of Scleropyrum wallichianum in Yunnan, the genetic variations of eighty-six germplasms from seven populations were analyzed by using AFLP markers. Eight pairs of AFLP primers with high and stable polymorphism were selected, amplified 1 728 DNA bands, of which 1 388 bands were polymorphic, accounting for 80.14%. The genetic diversity indexes at the species level were Na= 1.416, Ne=1.179, H=0.137, I=0.225, and at the population level were H=0.111, I=0.175. At similarity coefficient of 0.52, all germplasms could be divided into three groups. The population YW had abundant genetic diversity, and most of genetic variation existed within populations, while genetic variation among populations was 11.5% under confidence interval of 0.05. There was no significant correlation between genetic distance and geographical distance among populations(r=0.0323, P=0.5820). Therefore, in situ and ex situ protections would be proposed for increasing genetic diversity of S. wallichianum germplasms.
In order to understand the genetic diversity of Scleropyrum wallichianum in Yunnan, the genetic variations of eighty-six germplasms from seven populations were analyzed by using AFLP markers. Eight pairs of AFLP primers with high and stable polymorphism were selected, amplified 1 728 DNA bands, of which 1 388 bands were polymorphic, accounting for 80.14%. The genetic diversity indexes at the species level were Na= 1.416, Ne=1.179, H=0.137, I=0.225, and at the population level were H=0.111, I=0.175. At similarity coefficient of 0.52, all germplasms could be divided into three groups. The population YW had abundant genetic diversity, and most of genetic variation existed within populations, while genetic variation among populations was 11.5% under confidence interval of 0.05. There was no significant correlation between genetic distance and geographical distance among populations(r=0.0323, P=0.5820). Therefore, in situ and ex situ protections would be proposed for increasing genetic diversity of S. wallichianum germplasms.
引文
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[14] DOYLE J. DNA protocols for plants[M]//HEWITT G M, JOHNSTON AWB,YOUNGJPW.MolecularTechniquesinTaxonomy.Berlin:Springer, 1991:283–293. doi:10.1007/978-3-642-83962-7_18.
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[2]MORRISLJ,MARSHALLMO.Occurrenceofstearolicacidin Santalaceae seed oils[J]. Chem Ind, 1966, 12:460–461.
[3]WANG J P, LI J M, YU F L. Acetylenic acids from seed oil of Scleropyrumwallichianum[J]. Acta Bot Yunnan, 1992, 14(1):101–104.(in Chinese)
[4]WANGJR,LONGCL.Ethnobotanicalstudyoftraditionaledible plants of Jinuo nationality[J]. Acta Bot Yunnan, 1995, 17(2):161–168.(in Chinese)
[5]Editorial Committee of Oil Plants in China. Oil Plants in China[M].Beijing:Science Press, 1987:90–92.(in Chinese).
[6]WU Y, HE M Y, ZHANG F L, et al. Phenotype variations of seed in wildingScleropyrumwallichianum[J].JNEForUniv,2015,43(8):29–33.(in Chinese)
[7]WU Y, HE M Y, ZHANG F L, et al. Population variation of fatty acids fromtheseedswildoil-bearingtreeScleropyrumwallichianumin Yunnan[J]. J NE For Univ, 2015, 30(6):99–103.(in Chinese)doi:10.3969/j.issn.1001-7461.2015.06.17.
[8]QIN X B, GAO J H. Studies on genetic diversity for specialty kiwifruits fromsouthwesternregionsofChinausingAFLPsystem[J].JTrop SubtropBot,2013,21(4):315–322.(inChinese)doi:10.3969/j.issn.1005-3395.2013.04.004.
[9]ZHANGY,RANH,FENGY,etal.Studiesontherelationshipof newly published infra-species of Phyllostachys edulis by AFLP marker[J]. J Trop Subtrop Bot, 2016, 24(6):657–664.(in Chinese)doi:10.11926/j.issn.1005-3395.2016.06.009.
[10] RAPPOSELLIE,MELITOS,BARMINAGG,etal.AFLPfinger printing and essential oil profiling of cultivated and wild populations of Sardinian Salvia desoleana[J]. Genet Resour Crop Evol, 2015, 62(6):959–970. doi:10.1007/s10722-015-0263-1.
[11] LIU J Y, ZHAO P F, LIU X L, et al. Genetic diversity analysis on 68foreign sugarcane germplasms(Saccharum spp.)with AFLP technique[J]. J Hunan Agric Univ, 2013, 39(5):466–470.(in Chinese)
[12] WANGX,XINGSY,SUNLM,etal.GeneticdiversityofStyrax obassia Sieb. et Zucc. based on AFLP markers[J]. Biochem Syst Ecol,2015, 61:28–34. doi:10.1016/J.BSE.2015.04.024.
[13] WU Y, ZHANG F L, HE M Y, et al. Investigation of wild resource of oil tree Scleropyrum wallichianum in Yunnan[J]. Trop Agric Sci Technol,2015, 38(1):24–26.(in Chinese)doi:10.3969/j.issn.1672-450X.2015.01.007.
[14] DOYLE J. DNA protocols for plants[M]//HEWITT G M, JOHNSTON AWB,YOUNGJPW.MolecularTechniquesinTaxonomy.Berlin:Springer, 1991:283–293. doi:10.1007/978-3-642-83962-7_18.
[15] EXCOFFIERL,LAVALG,SCHNEIDERS.Arlequin(version3.0):An integrated software package for population genetics data analysis[J]. Evol Bioinform, 2005, 1:47–50. doi:10.1177/117693430500100003.
[16] MILLER M P. Tools for Population Genetic Analyses(TFPGA), Ver.1.3 A[CP/OL].[2018-01-10] http://www.marksgeneticsoftware.net/_vti_bin/shtml.exe/tfpga.htm?
[17] LEVIN D A, KERSTER H W. Gene flow in seed plants[M]//DOBZHANSKY T, HECHT M K, STEERE W C. Evolutionary Biology. Boston,MA:Springer, 1974:139–220. doi:10.1007/978-1-4615-944-2_5.