广州市腹泻患者Aichi病毒的检测及基因型
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摘要
目的了解广州市腹泻患者粪便中Aichi病毒的携带情况,并对其进行基因分析。方法在2015年至2018年,采集南方医科大学某附属医院门诊腹泻患者的粪便样本,利用PCR技术检测粪便中Aichi病毒。采用基因测序与序列比对的方法确认阳性样本,并且对阳性样本病毒序列进行基因进化分析,构建系统进化树。结果在1 002份腹泻患者粪便样本中,检测到2例Aichi病毒阳性标本,经基因分析一株病毒株为B基因型,另一株为A基因型。结论本研究首次在广东地区发现Aichi病毒,并首次在我国大陆地区检测到A基因型的Aichi病毒,但Aichi病毒总体检出率很低。
Objective To understand the prevalence and phylogenetic characteristics of Aichi Virus in fecalsamples from patients with diarrhea in Guangzhou City.MethodsWe used PCR detection methods to detect Aichiviruses in fecal samples from patients with diarrhea between 2015 and 2018.ResultsA total of 1 002 fecal sampleswere collected from a hospital in Guangzhou city, only 2 positive samples were detected and verified. One positivesample was clustered in Aichi virus genotype B, and the other was clustered in genotype A.ConclusionThe detec-tion rate of Aichi virus in Guangzhou was very low. This is the first study to report Aichi virus in Guangdong Prov-ince and the first to report type A Aichi virus in the Chinese mainland.
Objective To understand the prevalence and phylogenetic characteristics of Aichi Virus in fecalsamples from patients with diarrhea in Guangzhou City.MethodsWe used PCR detection methods to detect Aichiviruses in fecal samples from patients with diarrhea between 2015 and 2018.ResultsA total of 1 002 fecal sampleswere collected from a hospital in Guangzhou city, only 2 positive samples were detected and verified. One positivesample was clustered in Aichi virus genotype B, and the other was clustered in genotype A.ConclusionThe detec-tion rate of Aichi virus in Guangzhou was very low. This is the first study to report Aichi virus in Guangdong Prov-ince and the first to report type A Aichi virus in the Chinese mainland.
引文
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[15]柳彩霞,李奇凤,余亮,等.2011年乌鲁木齐地区腹泻患儿中7种病毒性病原分子流行病学调查[J].中华微生物学和免疫学杂志,2013,(7):545-549.
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[18]杨芬,孙立梅,郭丽丽,等.广东省2013年其他感染性腹泻流行病学特征研究[J].华南预防医学,2015,41(3):233-237.
[19]张振,李媛,路滟,等.2010-2015年广东省深圳市其他感染性腹泻监测分析[J].疾病监测,2017,32(10):836-841.
[20]LODDER W J,RUTJES S A,TAKUMI K,et al.Aichi virus in sewage and surface water,the Netherlands[J].Emerg Infect Dis,2013,19(8):1222-1230.
[21]HOWARD L M,MWAPE I,SIWINGWA M,et al.Norovirus infections in young children in Lusaka Province,Zambia:clinical characteristics and molecular epidemiology[J].BMC Infect Dis,2017,17(1):92.
[22]CHUNG N,WANG S M,SHEN C F,et al.Clinical and epidemiological characteristics in hospitalized young children with acute gastroenteritis in southern Taiwan:According to major pathogens[J].J Microbiol Immunol Infect,2017,50(6):915-922.
[23]何艳明,梁秉绍,姚淑雯,等.2 171例腹泻患儿轮状病毒和腺病毒检出率及流行特征[J].实用医学杂志,2017,33(11):1872-1875.
[2]BHAT S,KATTOOR J J,MALIK Y S,et al.Species C rotaviruses in children with diarrhea in india,2010-2013:a potentially neglected cause of acute gastroenteritis[J].Pathogens,2018,7(1):23.
[3]GAO W,LI G,LIU X,et al.The impact of“Child Care”intervention in rural Primary Health Care Program on prevalence of diarrhea among children less than 36 months of age in rural western China[J].BMC Pediatr,2018,18(1):228.
[4]TROEGER C,BLACKER B F,KHALIL I A,et al.Estimates of the global,regional,and national morbidity,mortality,and aetiologies of diarrhoea in 195 countries:a systematic analysis for the Global Burden of Disease Study 2016[J].Lancet Infect Dis,2018,13(11):1211-1228.
[5]YAMASHITA T,SUGIYAMA M,TSUZUKI H,et al.Application of a reverse transcription-PCR for identification and differentiation of Aichi virus,a new member of the Picornavirus family associated with gastroenteritis in humans[J].J Clin Microbiol,2000,38(8):2955-2961.
[6]CHUCHAONA W,KHAMRIN P,YODMEEKLIN A,et al.Detection and characterization of Aichi virus 1 in pediatric patients with diarrhea in Thailand[J].J Med Virol,2017,89(2):234-238.
[7]HAN T H,PARK S H,HWANG E S,et al.Detection of Aichi virus in South Korea[J].Arch Virol,2014,159(7):1835-1839.
[8]JONSSON N,WAHLSTROM K,SVENSSON L,et al.Aichi virus infection in elderly people in Sweden[J].Arch Virol,2012,157(7):1365-1369.
[9]VERMA H,CHITAMBAR S D,GOPALKRISHNA V.Circulation of Aichi virus genotype B strains in children with acute gastroenteritis in India[J].Epidemiol Infect,2011,139(11):1687-1691.
[10]AMBERT-BALAY K,LORROT M,BON F,et al.Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients[J].J Clin Microbiol,2008,46(4):1252-1258.
[11]SDIRI-LOULIZI K,HASSINE M,GHARBI-KHELIFI H,et al.Detection and genomic characterization of Aichi viruses in stool samples from children in Monastir,Tunisia[J].J Clin Microbiol,2009,47(7):2275-2278.
[12]吴冰珊,沈晓娜,王美爱,等.福建省首次检出Aichi病毒及其分子流行病学调查[J].中国人兽共患病学报,2011,27(2):101-103.
[13]窦爱燕.Aichi病毒在小儿腹泻中的研究进展[J].中国临床研究,2014,27(8):1007-1008.
[14]李博文,梁莎,谢金鑫,等.在兰州地区儿童粪便中检测Aichi virus[J].中华实验和临床病毒学杂志,2010,24(1):11-13.
[15]柳彩霞,李奇凤,余亮,等.2011年乌鲁木齐地区腹泻患儿中7种病毒性病原分子流行病学调查[J].中华微生物学和免疫学杂志,2013,(7):545-549.
[16]GOYER M,AHO L S,BOUR J B,et al.Seroprevalence distribution of Aichi virus among a French population in 2006-2007[J].Arch Virol,2008,153(6):1171-1174.
[17]RIBES J M,MONTAVA R,TELLEZ-CASTILLO C J,et al.Seroprevalence of Aichi virus in a Spanish population from 2007 to2008[J].Clin Vaccine Immunol,2010,17(4):545-549.
[18]杨芬,孙立梅,郭丽丽,等.广东省2013年其他感染性腹泻流行病学特征研究[J].华南预防医学,2015,41(3):233-237.
[19]张振,李媛,路滟,等.2010-2015年广东省深圳市其他感染性腹泻监测分析[J].疾病监测,2017,32(10):836-841.
[20]LODDER W J,RUTJES S A,TAKUMI K,et al.Aichi virus in sewage and surface water,the Netherlands[J].Emerg Infect Dis,2013,19(8):1222-1230.
[21]HOWARD L M,MWAPE I,SIWINGWA M,et al.Norovirus infections in young children in Lusaka Province,Zambia:clinical characteristics and molecular epidemiology[J].BMC Infect Dis,2017,17(1):92.
[22]CHUNG N,WANG S M,SHEN C F,et al.Clinical and epidemiological characteristics in hospitalized young children with acute gastroenteritis in southern Taiwan:According to major pathogens[J].J Microbiol Immunol Infect,2017,50(6):915-922.
[23]何艳明,梁秉绍,姚淑雯,等.2 171例腹泻患儿轮状病毒和腺病毒检出率及流行特征[J].实用医学杂志,2017,33(11):1872-1875.