木霉拌种剂对小麦根际土壤真菌群落多样性的影响
|
摘要
采用Illumina Miseq高通量测序平台,对木霉LTR-2拌种剂拌种小麦后不同发育时期小麦根际土壤真菌进行测序,分析群落丰富度、多样性以及结构。结果表明,木霉拌种处理小麦可以增加小麦根际真菌群落的丰富度,对真菌群落的均匀度影响不明显,但真菌群落的优势度有明显的增加;木霉拌种处理后小麦根际土壤真菌的菌群结构构成相近,没有发生明显变化,但各真菌类群所占比例发生了明显的变化,其中一些病原真菌如链格孢属、赤霉属、镰刀菌属所占比例较不拌种处理均有所降低。该研究为木霉菌LTR-2防治小麦土传病害的生防机制提供了理论依据。
The species richness,diversity and composition of fungal community in wheat rhizosphere soil at different developmental stages with Trichoderma LTR-2 seed dressing agent were analyzed using Illumina MiSeq high-throughput sequencing platform. The results demonstrated that Trichoderma treatment of wheat seed increased the species richness of fungal community in the rhizosphere,and the community dominance increased significantly,while no obvious changes in the community uniformity were observed. In addition,the community composition was found similar with and without Trichoderma treatment. However,the proportion of various fungal groups in the rhizosphere changed significantly with Trichoderma treatment,among which Alternaria spp.,Gibberella spp.,and Fusarium spp. were decreased compared to non-seed dressing treatment. All the results provide a theoretical basis for better understanding the biocontrol mechanism of Trichoderma sp. LTR-2 against wheat soil-borne diseases.
The species richness,diversity and composition of fungal community in wheat rhizosphere soil at different developmental stages with Trichoderma LTR-2 seed dressing agent were analyzed using Illumina MiSeq high-throughput sequencing platform. The results demonstrated that Trichoderma treatment of wheat seed increased the species richness of fungal community in the rhizosphere,and the community dominance increased significantly,while no obvious changes in the community uniformity were observed. In addition,the community composition was found similar with and without Trichoderma treatment. However,the proportion of various fungal groups in the rhizosphere changed significantly with Trichoderma treatment,among which Alternaria spp.,Gibberella spp.,and Fusarium spp. were decreased compared to non-seed dressing treatment. All the results provide a theoretical basis for better understanding the biocontrol mechanism of Trichoderma sp. LTR-2 against wheat soil-borne diseases.
引文
[1]JONES D L,HINSINGER P. Editorial:The rhizosphere:complex by design[J]. Plant and Soil,2008,312(1/2):1-6.
[2]BAKKER P A H M,DOORNBOS R F,CHRISTOS Z,et al. Induced systemic resistance and the rhizosphere microbiome[J]. The Plant Pathology Journal,2013,29(2):136-143.
[3]SANTHANAM R,LUU V T,WEINHOLD A,et al. Native root-associated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping[J]. PNAS,2015,112(36):E5013-E5020.
[4]AGRIOS G N. Plant Pathology[M]. 5th ed. San Diego,US:Academic Press,2005.
[5]杨韶勇,曹克强,朱之堉.中国小麦真菌病害种类及地理分布[J].河北农业大学学报,1997,20(2):6-11.
[6]杨合同,唐文华,RYDER M.木霉菌与植物病害的生物防治[J].山东科学,1999,12(4):7-15.
[7]朱廷恒,邢小平,孙顺娣.木霉T-97菌株对几种植物病原真菌的拮抗作用机制和温室防治试验[J].植物保护学报,2004,31(2):139-144.
[8]张瑾,张树武,徐秉良,等.长枝木霉菌抑菌谱测定及其抑菌作用机理研究[J].中国生态农业学报,2014,22(6):661-667.
[9]CAPORASO J G,LAUBER C L,WALTERS W A,et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample[J]. PNAS,2011,108(S1):4516-4522.
[10]黄世文,余柳青,段桂芳,等.禾长蠕孢菌和尖角突脐孢菌防治稗草的研究[J].植物病理学报,2005,35(1):66-72.
[11]台莲梅,郭永霞,张亚玲等.木霉生防菌对大豆幼苗的促生作用及对根腐病的防治效果[J].安徽农业科学,2013,41(11):4820-4821.
[12]谭兆赞,徐广美,刘可星,等.不同堆肥对番茄青枯病的防病效果及土壤微生物群落功能多样性的影响[J].华南农业大学学报,2009,30(2):10-14.
[13]韩腾,张立猛,高加明,等.枯草芽孢杆菌(Bacillus subtilis)Tpb55灌根与烟草根围细菌多样性变化的相关性[J].微生物学报,2016,56(5):835-845.
[2]BAKKER P A H M,DOORNBOS R F,CHRISTOS Z,et al. Induced systemic resistance and the rhizosphere microbiome[J]. The Plant Pathology Journal,2013,29(2):136-143.
[3]SANTHANAM R,LUU V T,WEINHOLD A,et al. Native root-associated bacteria rescue a plant from a sudden-wilt disease that emerged during continuous cropping[J]. PNAS,2015,112(36):E5013-E5020.
[4]AGRIOS G N. Plant Pathology[M]. 5th ed. San Diego,US:Academic Press,2005.
[5]杨韶勇,曹克强,朱之堉.中国小麦真菌病害种类及地理分布[J].河北农业大学学报,1997,20(2):6-11.
[6]杨合同,唐文华,RYDER M.木霉菌与植物病害的生物防治[J].山东科学,1999,12(4):7-15.
[7]朱廷恒,邢小平,孙顺娣.木霉T-97菌株对几种植物病原真菌的拮抗作用机制和温室防治试验[J].植物保护学报,2004,31(2):139-144.
[8]张瑾,张树武,徐秉良,等.长枝木霉菌抑菌谱测定及其抑菌作用机理研究[J].中国生态农业学报,2014,22(6):661-667.
[9]CAPORASO J G,LAUBER C L,WALTERS W A,et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample[J]. PNAS,2011,108(S1):4516-4522.
[10]黄世文,余柳青,段桂芳,等.禾长蠕孢菌和尖角突脐孢菌防治稗草的研究[J].植物病理学报,2005,35(1):66-72.
[11]台莲梅,郭永霞,张亚玲等.木霉生防菌对大豆幼苗的促生作用及对根腐病的防治效果[J].安徽农业科学,2013,41(11):4820-4821.
[12]谭兆赞,徐广美,刘可星,等.不同堆肥对番茄青枯病的防病效果及土壤微生物群落功能多样性的影响[J].华南农业大学学报,2009,30(2):10-14.
[13]韩腾,张立猛,高加明,等.枯草芽孢杆菌(Bacillus subtilis)Tpb55灌根与烟草根围细菌多样性变化的相关性[J].微生物学报,2016,56(5):835-845.