复方三草滴丸的急性毒性试验
|
摘要
目的实验考察复方三草滴丸的急性毒性,评价滴丸剂型的安全性。方法预实验:小白鼠禁食16h,不禁水,后随机分两组,每组10只,雌雄各5只。第一组按96g·kg~(-1)剂量一次性灌胃给药。第二组按192g·kg~(-1)给药,24h后,观察小鼠死亡数量。正式实验:20只小白鼠,随机分为4组:超高剂量组(464g·kg~(-1))、高剂量组(215g·kg~(-1))、中剂量组(100g·kg~(-1))、低剂量组(46.4g·kg~(-1)),雌鼠3只雄鼠2只。结果预实验96g·kg~(-1)组死2只,192g·kg~(-1)组死5只。正式实验超高剂量组(464g·kg~(-1))死5只,高剂量组(215g·kg~(-1))死4只、中剂量组(100g·kg~(-1))死1只。结论复方三草滴丸安全性大,LD_(50)为147g·kg~(-1),可信限区间:95.1～227g·kg~(-1)。
OBJECTIVE To study the acute toxicity of compound Three Herbs Droplet and evaluate the safety of Droplet.METHODS Pre-experimental:the mice were fasted for 16 hours,but they were not allowed to water.After the animals were randomly divided into 2 groups,10 in each group,5 males and 5 females.The first group was given bygavage at a dose of 96 g·kg~(-1).The second group was treated with 192 g·kg~(-1).After 24 hours,the number of mice died was observed.formal experiment:using 20 mice,the animals are randomly divided into 4 groups of ultra-high dose group(464 g·kg~(-1)),high dose group(215 g·kg~(-1)),medium dose group(100 g·kg~(-1)) In the low-dose group(46.4 g·kg~(-1)),3 females and 2 males mice.RESULTS Two mice died in the pre-experimental 96 g·kg~(-1) dose group,and five died in the 192 g·kg~(-1) dose group.Five high-dose groups(464 g·kg~(-1)) died in the formal experiment,four in the high-dose group(215 g·kg~(-1)),one in the middle-dose group(100 g·kg~(-1)).CONCLUSION The compound Three Herbs Droplet is safer with a LD_(50) of 147 g·kg~(-1) and a confidence interval of 95.1-227 g·kg~(-1).
OBJECTIVE To study the acute toxicity of compound Three Herbs Droplet and evaluate the safety of Droplet.METHODS Pre-experimental:the mice were fasted for 16 hours,but they were not allowed to water.After the animals were randomly divided into 2 groups,10 in each group,5 males and 5 females.The first group was given bygavage at a dose of 96 g·kg~(-1).The second group was treated with 192 g·kg~(-1).After 24 hours,the number of mice died was observed.formal experiment:using 20 mice,the animals are randomly divided into 4 groups of ultra-high dose group(464 g·kg~(-1)),high dose group(215 g·kg~(-1)),medium dose group(100 g·kg~(-1)) In the low-dose group(46.4 g·kg~(-1)),3 females and 2 males mice.RESULTS Two mice died in the pre-experimental 96 g·kg~(-1) dose group,and five died in the 192 g·kg~(-1) dose group.Five high-dose groups(464 g·kg~(-1)) died in the formal experiment,four in the high-dose group(215 g·kg~(-1)),one in the middle-dose group(100 g·kg~(-1)).CONCLUSION The compound Three Herbs Droplet is safer with a LD_(50) of 147 g·kg~(-1) and a confidence interval of 95.1-227 g·kg~(-1).
引文
[1]施婉玲,黄小宾.300例蛇伤患者流行特征分析[J].福建中医学院学报,2007,17(1):14-15.
[2]刘红燕.鬼针草属植物黄酮类物质超声提取工艺及含量测定[J].山东中医药大学学报,2012,36(5):443-444.
[3]于春.霍恩氏法与序贯法在急性经口毒性试验中应用研究[J].安徽农业科学,2017,45(21):7-8.
[4]何丽君,陈豪,郑婉玉,等.复方泽泻滴丸的急性毒性试验[J].中医临床研究,2013,22(5):41-42.
[5]黄伟.吴茱萸不同组分对小鼠急性毒性试验比较研究[J].中国药物警戒,2010,3(7):129-135.
[6]杨宝峰主编.药理学[M].北京:人民卫生出版社,2003,1-27.
[7]王梅兰,林向党,曹育,等.通脉灵大鼠长期毒性实验[J].海峡药学,2016,9(28):28-29.
[2]刘红燕.鬼针草属植物黄酮类物质超声提取工艺及含量测定[J].山东中医药大学学报,2012,36(5):443-444.
[3]于春.霍恩氏法与序贯法在急性经口毒性试验中应用研究[J].安徽农业科学,2017,45(21):7-8.
[4]何丽君,陈豪,郑婉玉,等.复方泽泻滴丸的急性毒性试验[J].中医临床研究,2013,22(5):41-42.
[5]黄伟.吴茱萸不同组分对小鼠急性毒性试验比较研究[J].中国药物警戒,2010,3(7):129-135.
[6]杨宝峰主编.药理学[M].北京:人民卫生出版社,2003,1-27.
[7]王梅兰,林向党,曹育,等.通脉灵大鼠长期毒性实验[J].海峡药学,2016,9(28):28-29.