产ε-聚赖氨酸白色链霉菌的化学诱变育种
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摘要
该研究以白色链霉菌(Streptomyces albus)BNCC 186223为原始菌株,使用硫酸二乙酯对其进行化学诱变,考察最佳诱变条件,并筛选高产ε-聚赖氨酸的菌株。结果表明,最佳诱变条件为硫酸二乙酯10μL/mL,诱变时间45min。在此最佳诱变条件下,获得一株高产ε-聚赖氨酸突变菌株DES-27,其ε-聚赖氨酸产量为2.90g/L,较未诱变前提高38.10%。因此,硫酸二乙酯的诱变作用能够显著提高ε-聚赖氨酸的产量。
Streptomyces albus BNCC 186223, as an original strain, was chemically mutagenized with diethyl sulfate. The optimal mutagenesis conditions were investigated and the strain with high yield ε-polylysine was screened. The results showed that the optimal mutagenesis conditions were diethyl sulfate 10 μl/ml and mutagenesis time 45 min. Under the optimal mutagenesis conditions, the mutant strain DES-27 with high yield ε-polylysine was obtained and the ε-polylysine production was 2.90 g/L, which was 38.10% higher than that before mutagenesis. Therefore, the mutagenic effect of diethyl sulfate could significantly increase the production of ε-polylysine.
Streptomyces albus BNCC 186223, as an original strain, was chemically mutagenized with diethyl sulfate. The optimal mutagenesis conditions were investigated and the strain with high yield ε-polylysine was screened. The results showed that the optimal mutagenesis conditions were diethyl sulfate 10 μl/ml and mutagenesis time 45 min. Under the optimal mutagenesis conditions, the mutant strain DES-27 with high yield ε-polylysine was obtained and the ε-polylysine production was 2.90 g/L, which was 38.10% higher than that before mutagenesis. Therefore, the mutagenic effect of diethyl sulfate could significantly increase the production of ε-polylysine.
引文
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[19]谢彪,唐晓芳,李镇江,等.产ε-聚赖氨酸菌补料分批发酵工艺的研究[J].食品与发酵科技,2018,54(3):76-81.
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[2]高彦祥.食品添加剂[M].北京:中国轻工业出版社,2016:44-50.
[3]贾士儒.生物防腐剂-ε-聚赖氨酸研究进展[J].发酵科技通讯,2005,34(3):22-25.
[4]KAHAR P,IWATA T,HIRAKI J,et al.Enhancement ofε-polylysine production by Streptomyces albulus strain 410 using p H control[J].J Biosci Bioeng,2001,91(2):190-194.
[5]陈玮玮,朱宏阳,徐虹.ε-聚赖氨酸高产菌株选育及分批发酵的研究[J].工业微生物,2007,37(2):28-30.
[6]李双双,吴振强,吴清平,等.产ε-赖氨酸白色链霉菌复合诱变选育研究[J].中国酿造,2010,29(12):108-111.
[7]LI S,LI F,CHEN X S,et al.Genomeshuffling enhancedε-poly-l-lysine production by improving glucose tolerance of Streptomyces graminearus[J].Appl Biochem Biotechnol,2012,166(2):414-423.
[8]ZONG H,ZHAN Y,LI X,et al.A new mutation breeding method for Streptomyces albulus by anatmospheric and room temperature plasma[J].Afr J Microbiol Res,2012,6(13):3154-3158.
[9]王昭君.土壤中产ε-聚赖氨酸菌株的筛选、发酵及其发酵产物提取[D].石河子:石河子大学,2018.
[10]李聪,扶教龙,施磊,等.ε-聚赖氨酸生产菌的硫酸二乙酯诱变及其发酵培养基优化[J].中国调味品,2018(3):34-40.
[11]王刚,杭锋,邢家溧,等.ε-聚赖氨酸生产菌白色链霉菌UN2-71生理特性及其发酵条件优化[J].乳业科学与技术,2013,36(6):1-5.
[12]杨玉红.ε-聚赖氨酸产生菌的筛选、鉴定及发酵的研究[D].沈阳:沈阳农业大学,2007.
[13]赖永勤.ε-聚赖氨酸菌种选育及合成途径的初步研究[D].成都:西南交通大学,2017.
[14]余明洁.高产ε-聚赖氨酸白色链霉菌的诱变育种与发酵优化[D].无锡:江南大学,2008.
[15]施磊.环境友好型防腐剂ε-聚赖氨酸生物合成工艺的研究[D].苏州:苏州科技大学,2016.
[16]程传荣.高产ε-聚赖氨酸白色链霉菌的原生质体诱变选育及发酵研究[D].无锡:江南大学,2009.
[17]陈旭升.ε-聚赖氨酸高产菌株选育与发酵过程优化[D].无锡:江南大学,2008.
[18]宗红.ε-聚赖氨酸高产菌株的选育及发酵生产的研究[D].杭州:浙江大学,2011.
[19]谢彪,唐晓芳,李镇江,等.产ε-聚赖氨酸菌补料分批发酵工艺的研究[J].食品与发酵科技,2018,54(3):76-81.
[20]ITZHAKI R F.Colorimetric method for estimating polylysine and polyarginine[J].Anal Biochem,1972,50(2):569-574.