不同中药总黄酮含量测定方法的选择研究
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摘要
微波辅助提取十种中药中的总黄酮,以芦丁为标准品,用五种不同的测定方法探讨不同中草药中总黄酮的含量,研究黄酮结构和测定方法之间的关系.结果表明:直接测定法更适合甘草、银杏叶和陈皮;NaNO_2-Al(NO_3)_3法更适合于山楂和黄芪;差示分光光度法更适合于野菊花、山楂、葛根和连翘;AlCl_3法更适合于陈皮;荧光光度法除山楂外,其它中草药测定均佳.根据总黄酮含量的测定方法的原理探讨不同中药中黄酮类物质的结构.
In order to investigate the best assay method of total flavonoids and the relationship between flavonoids structure and assay method,total flavonoids of ten traditional Chinese medicines were extracted by microwave technology and determined by different methods.The results showed that the direct methods were suitable for testing the total flavonoids of Glycyrrhiza uralensis,leaves of Ginkgo biloba and pericarpium citri reticulatae,the method of Na NO_2-Al(NO_3)_3 was suitable for Crataegus pinnatifida and Astragalus membranaceus,the method of differential spectrophotometry was suitable for Chrysanthemum indicum,Crataegus pinnatifida,Pueraria lobata,and Forsythia suspensa.The method of AlCl_3 was suitable for pericarpium citri reticulatae,and the method of fluorospectrophotometry was suitable for all apart from the hawthorn.The determination of total flavonoids in the Chinese medicines was affected by various factors,such as the types of flavonoids,the choice of measurement method,etc.
In order to investigate the best assay method of total flavonoids and the relationship between flavonoids structure and assay method,total flavonoids of ten traditional Chinese medicines were extracted by microwave technology and determined by different methods.The results showed that the direct methods were suitable for testing the total flavonoids of Glycyrrhiza uralensis,leaves of Ginkgo biloba and pericarpium citri reticulatae,the method of Na NO_2-Al(NO_3)_3 was suitable for Crataegus pinnatifida and Astragalus membranaceus,the method of differential spectrophotometry was suitable for Chrysanthemum indicum,Crataegus pinnatifida,Pueraria lobata,and Forsythia suspensa.The method of AlCl_3 was suitable for pericarpium citri reticulatae,and the method of fluorospectrophotometry was suitable for all apart from the hawthorn.The determination of total flavonoids in the Chinese medicines was affected by various factors,such as the types of flavonoids,the choice of measurement method,etc.
引文
[1]钟永祥,卢秀琼,邹永东,等.结肠康颗粒剂的制备及质量标准研究[J].中草药,1998,29(8):528-530
[2]王涛,江滨,曾元儿,等.四君子汤和煎液与单煎液中总黄铜的测定[J].时珍国医国药,2002,13(9):519-520
[3]欧敏锐,许小平,邓焱杰,等.福建产白花蛇舌草活性成分提取与含量测定[J].海峡医药,2004,16(2):43-45
[4]张磊,李辉敏.AB-8树脂分离提取复叶耳蕨中总黄酮含量测定[J].九江医学,2003,18(4):193-194
[5]古丽娜尔·夏依马尔旦.几种中草药中总黄酮含量的测定[J].新疆师范大学学报,2009,28(1):67-70
[6]穆宏磊,陈杭君,葛林梅,等.荧光光度法测定芦笋中总黄酮含量[J].中国食品学报,2010,2(10):201-205
[7]池玉梅,居羚,邓海山,等.分光光度测定总黄酮法的适用性[J].分析化学研究简报,2010,38(6):893-896
[8]何书美,刘敬兰.茶叶中总黄酮含量测定方法的研究[J].分析化学研究简报,2007,35(9):1365-1368
[9]任珊珊,包保全,毛婷,等.中药中总黄酮的含量测定方法研究进展[J].北方药,2015,12(3):112-115
[10]严赞开.紫外分光光度法测定植物黄酮含量的方法[J].食品研究与开发,2007,28(9):164-167
[11]鲁燕滨,王玉凤,陈林,等.两种测定水飞蓟胶囊中总黄酮含量方法的比较分[J].海南师范大学学报,2015,28(2):163-166
[2]王涛,江滨,曾元儿,等.四君子汤和煎液与单煎液中总黄铜的测定[J].时珍国医国药,2002,13(9):519-520
[3]欧敏锐,许小平,邓焱杰,等.福建产白花蛇舌草活性成分提取与含量测定[J].海峡医药,2004,16(2):43-45
[4]张磊,李辉敏.AB-8树脂分离提取复叶耳蕨中总黄酮含量测定[J].九江医学,2003,18(4):193-194
[5]古丽娜尔·夏依马尔旦.几种中草药中总黄酮含量的测定[J].新疆师范大学学报,2009,28(1):67-70
[6]穆宏磊,陈杭君,葛林梅,等.荧光光度法测定芦笋中总黄酮含量[J].中国食品学报,2010,2(10):201-205
[7]池玉梅,居羚,邓海山,等.分光光度测定总黄酮法的适用性[J].分析化学研究简报,2010,38(6):893-896
[8]何书美,刘敬兰.茶叶中总黄酮含量测定方法的研究[J].分析化学研究简报,2007,35(9):1365-1368
[9]任珊珊,包保全,毛婷,等.中药中总黄酮的含量测定方法研究进展[J].北方药,2015,12(3):112-115
[10]严赞开.紫外分光光度法测定植物黄酮含量的方法[J].食品研究与开发,2007,28(9):164-167
[11]鲁燕滨,王玉凤,陈林,等.两种测定水飞蓟胶囊中总黄酮含量方法的比较分[J].海南师范大学学报,2015,28(2):163-166