铝胁迫下八仙花HmVALT基因表达的差异
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摘要
【目的】了解八仙花HmVALT基因表达与耐铝能力的关系,揭示八仙花耐铝机理。【方法】以耐铝的八仙花品种H.macrophyllacv‘Coerulea’为材料,扩增获得HmVALT基因片段,利用半定量RT-PCR方法,分析在0,50,100,200,400,600μmol/L AlCl3胁迫下,八仙花根和叶中HmVALT基因的表达情况。【结果】各处理HmVALT基因在八仙花根和叶部均有表达,且根部的表达量高于叶部;铝胁迫浓度为400μmol/L时八仙花根和叶中HmVALT基因表达量最高,随后降低。【结论】HmVALT基因的表达与八仙花耐铝性密切相关。
【Objective】This experiment investigated the relationship between expression of HmVALT gene and aluminum resistance of Hydrangea macrophyllato provide basis for study on aluminum tolerant mechanism of H.macrophylla.【Method】The HmVALTgene segment was obtained from H.macrophylla cv‘Coerulea'via RT-PCR.Semi-quantitative RT-PCR was used to analyze the expression variation of HmVALTgene under aluminum stress with different concentrations of 0,50,100,200,400,and 600μmol/L.【Result】The expression of HmVALT gene was found in all treatments,and the expression level in root was higher than that in leaf.The highest expression was obtained with AlCl3 concentration of 400μmol/L,and then it decreased.【Conclusion】The expression of HmVALTgene was closely related to aluminum tolerance of H.macrophylla.
【Objective】This experiment investigated the relationship between expression of HmVALT gene and aluminum resistance of Hydrangea macrophyllato provide basis for study on aluminum tolerant mechanism of H.macrophylla.【Method】The HmVALTgene segment was obtained from H.macrophylla cv‘Coerulea'via RT-PCR.Semi-quantitative RT-PCR was used to analyze the expression variation of HmVALTgene under aluminum stress with different concentrations of 0,50,100,200,400,and 600μmol/L.【Result】The expression of HmVALT gene was found in all treatments,and the expression level in root was higher than that in leaf.The highest expression was obtained with AlCl3 concentration of 400μmol/L,and then it decreased.【Conclusion】The expression of HmVALTgene was closely related to aluminum tolerance of H.macrophylla.
引文
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[14]Larsen P B,Cancel J,Rounds M,et al.Arabidopsis ALS1encodes a root tip and stele localized half type ABC transporter required for root growth in an aluminum toxic environment[J].Planta,2007,225(6):1447-1458.
[15]Furukawa J,Yamaji N,Wang H,et al.An aluminum-activated citrate transporter in barley[J].Plant&Cell Physiology,2007,48(8):1081-1091.
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[18]魏跃,王永平,王全智,等.甜瓜属野生种铝胁迫诱导基因ChAI的克隆及序列分析[J].核农学报,2010,24(6):1286-1290.Wei Y,Wang Y P,Wang Q Z,et al.Cloning and sequence analysis of aluminum-induced gene ChAIfrom the wild CUCUNIS(Cucumis hystrix Chakr.)[J].Journal of Nuclear Agricultural Sciences,2010,24(6):1286-1290.
[19]Negishi T,Oshima K,Hattori M,et al.Tonoplast-and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant[J].PLoS One,2012,7(8):e43189.
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[21]Kinraide T.Identity of the rhizotoxic aluminum species[J].Plant and Soil,1991,134(1):167-178.
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[23]Zhu M J,Wang X C,Chen J,et al.The construction ofγ-TIP prokaryotic expression vector and preparation of theγ-TIP antibody[J].Acta Botanica Sinica,1999,41(11):1235-1238.
[24]Maurel C,Reizer J,Schroeder J I,et al.The vacuolar membrane proteinγ-TIP creates water specific channels in Xenopus oocytes[J].EMBO Journal,1993,12(6):2241-2247.
[25]Beebo A,Thomas D,Der C,et al.Life with and without AtTIP1.1,an Arabidopsis aquaporin preferentially localized in the apposing tonoplasts of adjacent vacuoles[J].Plant Molecular Biology,2009,70(1/2):193-209.
[26]Liu Q,Umeda M,Uchimiya H.Isolation and expression analysis of two rice genes encoding the major intrinsic protein[J].Plant Molecular Biology,1994,26(6):2003-2007.
[27]Chaumont F,Barrieu F,Herman E M,et al.Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation[J].Plant Physiology,1998,117(4):1143-1152.
[28]Jahn T P,Moller A L,Zeuthen T,et al.Aquaporin homologues in plants and mammals transport ammonia[J].FEBS Letters,2004,574(1/2/3):31-36.
[29]Kaldenhoff R,Eckert M.Features and function of plant aquaporins[J].Journal of Photochemistry and Photobiology,1999,52(1):1-6.
[2]Ryan P,Delhaize E,Jones D.Function and mechanism of organic anion exudation from plant roots[J].Annual Reviews of Plant Physiology,Plant Molecular Biology,2001,52(2):527-560.
[3]郭天荣,陈丽萍,冯其芳,等.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576.Guo T R,Chen L P,Feng Q F,et al.Effect of aluminum and cadmium treatments on the growth and antioxidant property of water spinach[J].Journal of Nuclear Agricultural Sciences,2015,29(3):571-576.
[4]Suhayda C G,Haug A.Organic acids reduce aluminum toxicity in maize root membranes[J].Physiologia Plantarum,1986,68(2):189-195.
[5]Ma J F,Ryan P R,Delhaize E.Aluminum tolerance in plants and the complexing role of organic acids[J].Trends in Plant Science,2001,6(6):273-278.
[6]Delhaize E,Ryan P R,Hebb D M,et al.Engineering high-level aluminum tolerance in barley with the ALMT1gene[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(42):15249-15254.
[7]莫丙波,沈春鹏,于智卫,等.铝对大豆根系柠檬酸合成与分泌的影响[J].生态环境学报,2009,18(3):1037-1041.Mo B B,Shen C P,Yu Z W,et al.Effect of aluminum on synthesis and secretion of citrate in soybean roots[J].Ecology and Environmental Sciences,2009,18(3):1037-1041.
[8]左方华,凌桂芝,唐新莲,等.铝胁迫诱导柱花草根系分泌柠檬酸[J].中国农业科学,2010,43(1):59-64.Zuo F H,Ling G Z,Tang X L,et al.Al stress-induced citrate secretion from roots in Stylosanthes[J].Scientia Agricultura Sinica,2010,43(1):59-64.
[9]Ryan P R,Tyerman S D,Sasaki T,et al.The identification of aluminum-resistance genes provides opportunities for enhancing crop production on acid soils[J].Journal of Experimental Botany,2011,62(1):9-20.
[10]Matsumoto H,Hirasawa E,Morimura S,et al.Localization of aluminum in tea leaves[J].Plant&Cell Physiology,1976,17(3):627-631.
[11]Morita A,Horie H,Fujii Y,et al.Chemical forms of aluminum in xylem sap of tea plants(Camellia sinensis L.)[J].Phytochemistry,2004,65(20):2775-2780.
[12]Ma J F,Zheng S J,Hiradate S,et al.Detoxifying aluminum with buckwheat[J].Nature,1997b,390(6660):569-570.
[13]Sasaki T,Yamamoto Y,Ezaki B,et al.A wheat gene encoding an aluminum-activated malate transporter[J].Plant Journal,2004,37(5):645-653.
[14]Larsen P B,Cancel J,Rounds M,et al.Arabidopsis ALS1encodes a root tip and stele localized half type ABC transporter required for root growth in an aluminum toxic environment[J].Planta,2007,225(6):1447-1458.
[15]Furukawa J,Yamaji N,Wang H,et al.An aluminum-activated citrate transporter in barley[J].Plant&Cell Physiology,2007,48(8):1081-1091.
[16]Maron L,Pineros M A,Guimaraes C T,et al.Two functionally distinct members of the MATE(multi-drug and toxic compound extrusion)family of transporters potentially underlie two major aluminum tolerance QTLs in maize[J].Plant Journal for Cell&Molecular Biology,2010,61(5):728-740.
[17]Famoso A N,Clark R T,Shaff J E,et al.Development of a novel aluminum tolerance phenotyping platform used for comparisons of cereal aluminum tolerance and investigations into rice aluminum tolerance mechanisms[J].Plant Physiology,2010,153(4):1678-1691.
[18]魏跃,王永平,王全智,等.甜瓜属野生种铝胁迫诱导基因ChAI的克隆及序列分析[J].核农学报,2010,24(6):1286-1290.Wei Y,Wang Y P,Wang Q Z,et al.Cloning and sequence analysis of aluminum-induced gene ChAIfrom the wild CUCUNIS(Cucumis hystrix Chakr.)[J].Journal of Nuclear Agricultural Sciences,2010,24(6):1286-1290.
[19]Negishi T,Oshima K,Hattori M,et al.Tonoplast-and plasma membrane-localized aquaporin-family transporters in blue hydrangea sepals of aluminum hyperaccumulating plant[J].PLoS One,2012,7(8):e43189.
[20]Wudick M M,Luu D T,Maurel C A.Look inside:localization patterns and functions of intracellular plant aquaporins[J].New Phytologist,2009,184(2):289-302.
[21]Kinraide T.Identity of the rhizotoxic aluminum species[J].Plant and Soil,1991,134(1):167-178.
[22]张晓娟,张林生,杨颖.水分胁迫下小麦类脱水素基因表达的半定量RT-PCR分析[J].西北植物学报,2007,27(11):2158-2162.Zhang X J,Zhang L S,Yang Y.Analysis of wheat dehydrin like gene during water stress condition by semi-quantitative RT-PCR[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(11):2158-2162.
[23]Zhu M J,Wang X C,Chen J,et al.The construction ofγ-TIP prokaryotic expression vector and preparation of theγ-TIP antibody[J].Acta Botanica Sinica,1999,41(11):1235-1238.
[24]Maurel C,Reizer J,Schroeder J I,et al.The vacuolar membrane proteinγ-TIP creates water specific channels in Xenopus oocytes[J].EMBO Journal,1993,12(6):2241-2247.
[25]Beebo A,Thomas D,Der C,et al.Life with and without AtTIP1.1,an Arabidopsis aquaporin preferentially localized in the apposing tonoplasts of adjacent vacuoles[J].Plant Molecular Biology,2009,70(1/2):193-209.
[26]Liu Q,Umeda M,Uchimiya H.Isolation and expression analysis of two rice genes encoding the major intrinsic protein[J].Plant Molecular Biology,1994,26(6):2003-2007.
[27]Chaumont F,Barrieu F,Herman E M,et al.Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation[J].Plant Physiology,1998,117(4):1143-1152.
[28]Jahn T P,Moller A L,Zeuthen T,et al.Aquaporin homologues in plants and mammals transport ammonia[J].FEBS Letters,2004,574(1/2/3):31-36.
[29]Kaldenhoff R,Eckert M.Features and function of plant aquaporins[J].Journal of Photochemistry and Photobiology,1999,52(1):1-6.