外源IAA增强丹波黑大豆抗铝性的生理机制
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摘要
以铝耐受型丹波黑大豆(RB)幼苗为供试材料,考察了不同浓度铝胁迫下添加外源IAA对RB根尖内源IAA、H2O2、MDA、铝含量、柠檬酸分泌量以及质膜H+-ATPase活性的影响,探讨外源IAA增强RB抗铝能力的生理机制。结果显示:(1)经25、50和200μmol·L-1 AlCl3胁迫处理24h后,RB根尖IAA含量、柠檬酸分泌量和质膜H+-ATPase活性均表现为先上升后下降的趋势,而H2O2、MDA和根尖铝含量却随着铝处理浓度的增加呈显著上升趋势。(2)与单独25、50和200μmol·L-1 AlCl3胁迫处理相比,添加50μmol·L-1外源IAA后使得RB根尖铝、MDA和H2O2含量均显著降低,同时使RB根尖柠檬酸分泌量分别相应增加到单独处理的2.39、1.73和6.85倍,且其相应的质膜H+-ATPase活性也增加了1.09、1.74和1.45倍。研究表明,外源IAA能通过增强丹波黑大豆根尖柠檬酸的分泌量和质膜H+-ATPase活性来提高其对铝胁迫的抗性,明显缓解铝毒害作用。
In this study,the Tamba Black soybean(RB)was used as the experimental material to analysis the physiological mechanism of exogenous IAA increasing Al resistance by investigation the effect of exogenous IAA on the contents of endogenous IAA,H2 O2,MDA,Al,citrate exudation and the activities of the plasma membrane(PM)H+-ATPase under Al stress.The results showed that after exposure to 25,50and 200μmol·L-1 AlCl3 for 24h,the contents of endogenous IAA,citrate exudation and the activities of the PM H+-ATPase in RB roots were firstly promoted,then decreased.However,the contents of H2 O2 and MDA were increased significantly with Al concentrations increasing.Additionally,the contents of Al,MDA and H2 O2 in RB roots were significantly decreased while 50μmol·L-1 IAA was added to 25,50and 200 μmol·L-1 Al treatment solutions.Furthermore,the citrate exudation increased approximately 2.39,1.73 and 6.85-fold and the activities of the PM H+-ATPase enhanced approximately 1.09,1.74and 1.45-fold than that of only Al treatment in RB roots,respectively.Taken together,our results indicated that exogenous application of IAA significantly enhanced Al resistance of RB by enhancement of citrate exudation and the PM H+-ATPase activity under Al stress.
In this study,the Tamba Black soybean(RB)was used as the experimental material to analysis the physiological mechanism of exogenous IAA increasing Al resistance by investigation the effect of exogenous IAA on the contents of endogenous IAA,H2 O2,MDA,Al,citrate exudation and the activities of the plasma membrane(PM)H+-ATPase under Al stress.The results showed that after exposure to 25,50and 200μmol·L-1 AlCl3 for 24h,the contents of endogenous IAA,citrate exudation and the activities of the PM H+-ATPase in RB roots were firstly promoted,then decreased.However,the contents of H2 O2 and MDA were increased significantly with Al concentrations increasing.Additionally,the contents of Al,MDA and H2 O2 in RB roots were significantly decreased while 50μmol·L-1 IAA was added to 25,50and 200 μmol·L-1 Al treatment solutions.Furthermore,the citrate exudation increased approximately 2.39,1.73 and 6.85-fold and the activities of the PM H+-ATPase enhanced approximately 1.09,1.74and 1.45-fold than that of only Al treatment in RB roots,respectively.Taken together,our results indicated that exogenous application of IAA significantly enhanced Al resistance of RB by enhancement of citrate exudation and the PM H+-ATPase activity under Al stress.
引文
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[14]OUZOUNIDOU G,ILIAS I.Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity[J].Biologia Plantarum,2005,49(2):223-228.
[15]WU K H,XIAO S Q,CHEN Q,et al.Changes in the activity and transcription of antioxidant enzymes in response to Al stress in black soybeans[J].Plant Molecular Biology Reporter,2013,31(1):141-150.
[16]WEI A L(魏爱丽),CHEN Y ZH(陈云昭).Effect of IAA on soybean seedlings membrance injury and salt resistance[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2000,20(3):410-414(in Chinese).
[17]MA J F,FURUKAWA J.Recent progress in the research of external Al detoxification in higher plants:a mini review[J].Journal of Inorganic Biochemistry,2003,97:46-51.
[18]CHEN M(陈梅),CHEN Y H(陈亚华),SHEN ZH G(沈振国),et al.Alleviation of aluminum toxicity in wheat seedlings exogenous organic acids[J].Journal of Plant Physiology and Molecular Biology,2003,29(4):281-288(in Chinese).
[19]YANG Y,WANG Q L,GENG M J,et al.Effect of indole-3-acetic acid on aluminum-induced efflux of malic acid from wheat(Triticum aestivum L)[J].Plant Soil,2011,346:215-230.
[20]YANG Y,WANG Q L,GENG M J,et al.Effect of auxin on Al-induced malic acid efflux in wheat with differential Al tolerance[J].Scientia Agricultura Sinica,2010,43(10):2 016-2 022.
[2]YI J X(易杰祥),LL X(吕亮雪),LIU G D(刘国道).Research on soil acidification and acidic soil’s melioration[J].Journal of South China University of Tropical Agriculture,2006,12(1):23-28(in Chinese).
[3]KIM Y S,PARK W,NIAN H,et al.Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean[J].Soil Science&Plant Nutrition,2010,56:140-149.
[4]CHEN Q,WU K H,ZHANG Y N,et al.Physiological and molecular responses of broad bean(Vicia faba L.)to aluminum stress[J].Acta Physiologiae Plantarum,2012,34:2 251-2 263.
[5]GUO C L,CHEN Q,ZHAO L W,et al.Al-enhanced expression and interaction of 14-3-3proteinand plasma membrane H+-ATPase is related to Al-induced citrate secretion in an Al-resistant black soybean[J].Plant Molecular Biology Reporter,2013,31:1 012-1 024.
[6]XU W F,JIA L G,SHI W M,et al.Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress[J].New Phytol.,2013,197:139-150.
[7]SHEN H,CHEN J,WANG Z,et al.Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation[J].Journal of Experimental Botany,2006,57:1 353-1 362.
[8]WANG P(王平),CHEN Q(陈奇),CHEN D J(陈东杰),et al.Effect of exogenous IAA on root growth of black soybean under aluminum stress[J].Soybean Science,2013,32(5):650-654(in Chinese).
[9]LIPTION D S,BLANCHAR R W,BLEVINS D G.Citrate,malate,and succinate concentration in exudates from P-sufficient and P-stressed Medicago sativa L.seedlings[J].Plant Physiology,1987,85:315-317.
[10]CHEN Q,ZHANG X D,WANG S S,et al.Transcriptional and physiological changes of alfalfa in response to aluminum stress[J].Journal of Agricultural Science,2011,149:737-751.
[11]张志良,翟伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003:274-277.
[12]GAY C A,GEBICKI J M.Measurement of carbohydrates in plant extracts by anthrone[J].Biochem.J.,1954,57(3):508-514.
[13]SHEN H,HE L F,SASAKI T,et al.Citrate secretion coupled with the modulation of soybean root tip under aluminum stress.Up-regulation of transcription,translation,and threonine-oriented phosphorylation of plasma membrane H+-ATPase[J].Plant Physiology,2005,138:287-296.
[14]OUZOUNIDOU G,ILIAS I.Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity[J].Biologia Plantarum,2005,49(2):223-228.
[15]WU K H,XIAO S Q,CHEN Q,et al.Changes in the activity and transcription of antioxidant enzymes in response to Al stress in black soybeans[J].Plant Molecular Biology Reporter,2013,31(1):141-150.
[16]WEI A L(魏爱丽),CHEN Y ZH(陈云昭).Effect of IAA on soybean seedlings membrance injury and salt resistance[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2000,20(3):410-414(in Chinese).
[17]MA J F,FURUKAWA J.Recent progress in the research of external Al detoxification in higher plants:a mini review[J].Journal of Inorganic Biochemistry,2003,97:46-51.
[18]CHEN M(陈梅),CHEN Y H(陈亚华),SHEN ZH G(沈振国),et al.Alleviation of aluminum toxicity in wheat seedlings exogenous organic acids[J].Journal of Plant Physiology and Molecular Biology,2003,29(4):281-288(in Chinese).
[19]YANG Y,WANG Q L,GENG M J,et al.Effect of indole-3-acetic acid on aluminum-induced efflux of malic acid from wheat(Triticum aestivum L)[J].Plant Soil,2011,346:215-230.
[20]YANG Y,WANG Q L,GENG M J,et al.Effect of auxin on Al-induced malic acid efflux in wheat with differential Al tolerance[J].Scientia Agricultura Sinica,2010,43(10):2 016-2 022.