FISH法对肺腺癌穿刺标本EML4-ALK融合基因的检测
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摘要
目的探讨采用肺腺癌穿刺活检标本检测棘皮动物微管相关类蛋白4(EML4)与间变性淋巴瘤激酶(ALK)融合基因(EML4-ALK)的可行性。方法对20例肺腺癌经皮肺穿刺标本应用荧光原位杂交(FISH)法检测EML4-ALK融合基因。结果20例标本中,19例通过FISH检测成功,检测成功率为95.00%。其中阳性2例,阳性率为10.00%。结论肺腺癌穿刺活检标本可用作检测EML4-ALK融合基因,为靶向治疗提供参考依据。
Objective To test the feasibility of detecting echinoderm microtubule related protein 4(EML4)and anaplastic lymphoma kinase(ALK)fusion gene(EML4-ALK)in lung adenocarcinoma biopsy samples.Methods Fluorescence in situ hybridization(FISH)was performed for 20 lung adenocarcinoma biopsy samples to detect EML4-ALK fusion gene.Results FISH assay was successful for 19 of the 20 samples,with a success rate of 95%.2 of the 20 cases(10%)was positive for EML4-ALK fusion gene.Conclusion Lung adenocarcinoma biopsy specimens can be used for detection of EML4-ALK fusion gene,which provides references for targeted therapy of this disease.
Objective To test the feasibility of detecting echinoderm microtubule related protein 4(EML4)and anaplastic lymphoma kinase(ALK)fusion gene(EML4-ALK)in lung adenocarcinoma biopsy samples.Methods Fluorescence in situ hybridization(FISH)was performed for 20 lung adenocarcinoma biopsy samples to detect EML4-ALK fusion gene.Results FISH assay was successful for 19 of the 20 samples,with a success rate of 95%.2 of the 20 cases(10%)was positive for EML4-ALK fusion gene.Conclusion Lung adenocarcinoma biopsy specimens can be used for detection of EML4-ALK fusion gene,which provides references for targeted therapy of this disease.
引文
[1]梁小龙,王孟昭,张静,等.检测肺腺癌活检标本间变性淋巴瘤激酶蛋白表达和基因融合[J].中华肿瘤杂志,2014,36(7):501-504.
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[3]郭易楠,岳文涛.EML4-ALK融合基因在肿瘤中的研究进展[J].国际肿瘤学杂志,2014,41(7):484-487.
[4]Sasaki T,Rodig SJ,Chirieac LR,et al.The biology and treatment of EML4-ALK non-small cell lung cancer[J].Eur J Cancer,2010,46(10):1773-1780.
[5]王梦,杨继元,李军川,等.EML4-ALK在非小细胞肺癌中的表达及临床意义[J].临床肿瘤学杂志,2013,18(8):688-690.
[6]Gridelli C,Peters S,Sgambato A,et al.ALK inhibitors in the treatment of advanced NSCLC[J].Cancer Treat Rev,2014,40(2):300-306.
[2]张邢松,魏万里.肺癌组织中EML4-ALK基因表达的研究进展[J].现代肿瘤医学,2014,22(5):1226-1230.
[3]郭易楠,岳文涛.EML4-ALK融合基因在肿瘤中的研究进展[J].国际肿瘤学杂志,2014,41(7):484-487.
[4]Sasaki T,Rodig SJ,Chirieac LR,et al.The biology and treatment of EML4-ALK non-small cell lung cancer[J].Eur J Cancer,2010,46(10):1773-1780.
[5]王梦,杨继元,李军川,等.EML4-ALK在非小细胞肺癌中的表达及临床意义[J].临床肿瘤学杂志,2013,18(8):688-690.
[6]Gridelli C,Peters S,Sgambato A,et al.ALK inhibitors in the treatment of advanced NSCLC[J].Cancer Treat Rev,2014,40(2):300-306.