小鼠肥胖实验模型建立及其对肝脏Apo-A1和Apo-B基因表达的影响
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摘要
研究高脂饮食对小鼠体重、血清脂质及肝脏组织载脂蛋白A1 (Apo-A1)和载脂蛋白B (Apo-B)基因表达的影响,从而探讨Apo-A1和Apo-B基因在小鼠肥胖发生中的作用。选用20只体重相近的8周龄昆明雌性小鼠随机分为高脂组和正常组,分别给予高脂饲料与标准饲料,试验期为12周,称重并检测血清三酰甘油(TG)和总胆固醇(TC)水平,取肝脏组织,通过荧光定量PCR测定Apo-B和Apo-A1基因表达,并分析Apo-B/Apo-A1比值变化情况。结果发现与正常组相比,高脂组小鼠体重显著增加(P<0. 05),血清TC水平显著高于正常组(P<0. 05),而血清TG水平无明显变化。另外,高脂饮食处理导致小鼠肝脏组织Apo-A1基因表达量显著升高(P<0. 05),且高脂饮食组Apo-B/Apo-A1比值显著低于正常饮食组(P<0. 05)。研究表明,本研究小鼠肥胖实验模型建模成功,且Apo-A1基因在肝脏脂类代谢中可能发挥重要调节作用,但具体机制有待深入研究。
The present study was to investigate the effect of high-fat diet on the weight,serum lipids,the expression of the apolipoprotein-A1(Apo-A1) and apolipoprotein-B(Apo-B) genes in the liver tissue of mice,so as to illustrate the role of Apo-A1 and Apo-B in obesity. Twenty eight-week-old Kunming female mice with similar body weight were used and randomly divided into the high-fat group and the control group,the former given high-fat diet and the latter standard diet,for 12 weeks. The serum triglyceride(TG) and total cholesterol(TC) levels,together with the weight,were measured. Also,the expressions of Apo-B and Apo-A1 in liver tissues were detected by fluorescence quantitative PCR,and then the change of the Apo-B/Apo-A1 ratio was analyzed. The results showed that,compared with the control group,the weight of the mice in the high-fat group increased significantly(P<0. 05),the serum TC level was also significantly higher(P<0. 05),but the serum TG level did not change. In addition,the expression of the Apo-A1 gene in liver tissue was significantly increased by high-fat diet(P< 0. 05),and the ratio of Apo-B/Apo-A1(0. 38 + 0. 09) in the high-fat group was significantly lower than that in the control group(0. 83 + 0. 19)(P< 0. 05). To conclude,the obesity model of mice in this study was successfully established,and the Apo-A1 gene might play an important role in regulating liver lipid metabolism.
The present study was to investigate the effect of high-fat diet on the weight,serum lipids,the expression of the apolipoprotein-A1(Apo-A1) and apolipoprotein-B(Apo-B) genes in the liver tissue of mice,so as to illustrate the role of Apo-A1 and Apo-B in obesity. Twenty eight-week-old Kunming female mice with similar body weight were used and randomly divided into the high-fat group and the control group,the former given high-fat diet and the latter standard diet,for 12 weeks. The serum triglyceride(TG) and total cholesterol(TC) levels,together with the weight,were measured. Also,the expressions of Apo-B and Apo-A1 in liver tissues were detected by fluorescence quantitative PCR,and then the change of the Apo-B/Apo-A1 ratio was analyzed. The results showed that,compared with the control group,the weight of the mice in the high-fat group increased significantly(P<0. 05),the serum TC level was also significantly higher(P<0. 05),but the serum TG level did not change. In addition,the expression of the Apo-A1 gene in liver tissue was significantly increased by high-fat diet(P< 0. 05),and the ratio of Apo-B/Apo-A1(0. 38 + 0. 09) in the high-fat group was significantly lower than that in the control group(0. 83 + 0. 19)(P< 0. 05). To conclude,the obesity model of mice in this study was successfully established,and the Apo-A1 gene might play an important role in regulating liver lipid metabolism.
引文
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[2]刘桂,殷亮,王晓慧,等.高脂饮食诱导的肥胖与肥胖抵抗大鼠肝FAS和ACAT-2的蛋白表达差异[J].上海体育学院学报,2014,38(6):105-109.
[3]那立欣,赵丹,宁华,等.减肥功能实验动物模型的改良[J].卫生研究,2010,39(2):162-164.
[4]靖俊,丁宁,丁晓萌,等.高脂饮食对雄性小鼠生殖功能和睾丸内氧化低密度脂蛋白变化的影响[J].医学研究生学报,2016,29(2):133-137.
[5] Zhang B,Pu S,Zhang W,et al. Sex differences in risk factors,etiology,and short-term outcome of cerebral infarction in young patients[J]. Atherosclerosis,2011,216(2):420-425.
[6]屈雪莹,车琳.高密度脂蛋白在心血管代谢风险中的研究[J].国际心血管病杂志,2010,37(2):81-84.
[7]熊胜.鹅Lb-FABP基因克隆及填饲对鹅Lb-FABP、Apo-B和Apo-A1基因表达的影响[D].南京:南京农业大学,2013.
[8]陈玉熹,缪心军,蔡平平.载脂蛋白B与进展性脑梗死的相关性探讨[J].实用老年医学,2006,20(5):350-351.
[9]谭兴容,屈春梅,方超,等. ApoA1、Apo B与糖尿病血管并发症的关系[J].重庆医学,2011,40(13):1314-1316.
[10]葛春莲,尹霞,张涵亮,等. APO-A1、APO-B及其比值在诊断和治疗动脉粥样硬化中的临床意义[J].吉林大学学报(医学版),2003(2):225.
[11] Meisinger C,Loewel H,Mraz W,et al. Prognostic value of apolipoprotein B and A-I in the prediction of myocardial infarction in middle-aged men and women:results from the MONICA/KORA Augsburg cohort study[J]. Eur Heart J,2005,26(3):271-278.
[12] Walldius G,Jungner I,Holme I,et al. High apolipoprotein B,low apolipoprotein A-I,and improvement in the prediction of fatal myocardial infarction(AMORIS study):a prospective study[J]. Lancet,2001,358(9298):2026-2033.
[13] Yusuf S,Hawken S,Ounpuu S,et al. Effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries(the INTERHEART study):case-control study[J]. Lancet,2004,364(9438):937-952.
[14] Ley SH,Harris SB,Connelly PW,et al. Association of apolipoprotein B with incident type 2 diabetes in an aboriginal Canadian population[J]. Clin Chem,2010,56(4):666-670.
[15] Sierra JJ,Romero CA,Somers VK,et al. ApoB/apo A-I ratio:an independent predictor of insulin resistance in US non-diabetic subjects[J]. Eur Heart J,2007,28(21):2637-2643.
[16] Lee YH,Choi SH,Lee KW,et al. Apolipoprotein B/A1 ratio is associated with free androgen index and visceral adiposity and may be an indicator of metabolic syndrome in male children and adolescents[J]. Clin Endocrinol(Oxf),2011,74(5):579-586.