通腑泻肺方对ALI/ARDS大鼠AQP1/a-ENaC表达的影响
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摘要
[目的]探讨通腑泻肺方对ALI/ARDS大鼠肺水肿的可能作用机制。[方法]27只大鼠按体质量随机分为空白组、模型组、中药组,每组9只。模型组与中药组尾静脉注射内毒素8 mg/kg,空白组大鼠静脉注射生理盐水。中药组在造模完成后给予通腑泻肺中药灌胃,每次0.01 m L/g,每日1次,共7 d,空白组与模型组予等量生理盐水灌胃。通过病理切片观察各组大鼠肺组织损伤情况,免疫组化染色方法检测肺组织水通道蛋白1(AQP1)和上皮细胞钠通道(a-ENa C)表达。[结果]模型组大鼠肺组织中AQP1和a-ENa C表达水平显著下降(P<0.01);与模型组相比,中药组AQP1和a-ENa C表达显著升高(P<0.05)。[结论]通腑泻肺方能增强ALI/ARDS大鼠AQP1和a-ENa C表达而促进其肺部水液代谢,从而发挥其改善肺水肿的作用。
[Objective] To investigate the possible mechanism of Tongfu Xiefei Decoction on pulmonary edema in ALI/ARDS rats.[Methods] The 27 rats were randomly divided into blank group, model group and traditional Chinese medicine group. In the model group and the traditional Chinese medicine group, endotoxin was injected intravenously at 8 mg/kg, and the rats in the blank group were injected with normal saline. Chinese medicine group after the completion of modeling to give Tongfu laxative Chinese medicine gavage,each 0.01 m L/g, 1 times/d, a total of 7 days, the blank group and the model group to the same amount of normal saline. The expression of AQP1 and a-ENa C in lung tissue were detected by immunohistochemical staining. [Results] The expression of AQP1 and a-ENa C protein in lung tissue of model group decreased significantly(P<0.01). Compared with model group, the expression of AQP1 and a-ENa C protein in TCM group was significantly higher than that in model group(P<0.05). [Conclusion] Tongluo diarrhea can enhance ALI/ARDS rats AQP1 and a-ENa C protein expression and regulation of its water metabolism, so as to play its role in improving pulmonary edema.
[Objective] To investigate the possible mechanism of Tongfu Xiefei Decoction on pulmonary edema in ALI/ARDS rats.[Methods] The 27 rats were randomly divided into blank group, model group and traditional Chinese medicine group. In the model group and the traditional Chinese medicine group, endotoxin was injected intravenously at 8 mg/kg, and the rats in the blank group were injected with normal saline. Chinese medicine group after the completion of modeling to give Tongfu laxative Chinese medicine gavage,each 0.01 m L/g, 1 times/d, a total of 7 days, the blank group and the model group to the same amount of normal saline. The expression of AQP1 and a-ENa C in lung tissue were detected by immunohistochemical staining. [Results] The expression of AQP1 and a-ENa C protein in lung tissue of model group decreased significantly(P<0.01). Compared with model group, the expression of AQP1 and a-ENa C protein in TCM group was significantly higher than that in model group(P<0.05). [Conclusion] Tongluo diarrhea can enhance ALI/ARDS rats AQP1 and a-ENa C protein expression and regulation of its water metabolism, so as to play its role in improving pulmonary edema.
引文
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[2]Borgnia M,Nielsen S,Engel A,et al.Cellular and molecularbiology of the Aquaporin water channel[J].Annu Rev Biochem,1999,68:425-458.
[3]Borok Z,Liebler JM,Lubman RL,et al.Na transportproteins are expressed by rat alveolar epithelial type I cells[J].Am J Physiol Lung Cell Mol Physio,2002,282(4):L599-L608.
[4]Talbot CL,Bosworth DG,Briley EL,et al.Quantitationand localization of ENa C subunit expression in fetal,new-born,and adult mouse lung[J].Am J Respir Cell Mol Bi-ol,1999,20(3):398-406.
[5]Johnson ER,Matthay MA.Acute lung injury:epi-demiology,pathogenesis,and treatment[J].J Aerosol Med Pulm Drug Deliv,2010,23(4):243.
[6]Ablimit A,Hasan B,Lu W,et al.Changes in waterchannel aquaporin 1 and aquaporin 5 in the smallairways and the alveoli in a rat asthma model[J].Micron,2013,45:68-73.
[7]Bai C,Fukuda N,Song Y,et al.Lung fluid transport in aquapor-in-1 and aquaporin-4 knockout mice[J].J Clin Invest,1999,103(4):555-561.
[8]Matalon S,O’Brodovich H.Sodium channels in alveolarepithelial cells:molecular characterization,biophysicalproperties,and physiological significance[J].Annu Rev Physiol,1999,61:627-661.
[9]Chen HI,Yeh DY,Liou HL,et al.Insulin attenuates endotoxininduced acute lung injury in conscious rats[J].Crit Care Med.2006,34(3):758-764.
[10]Hummler E,Barker P,Gatzy J,et al.Early death due todefective neonatal lung liquid clearance in alpha-ENa C-de-ficient mice[J].Nat Genet,1996,12(3):325-328.
[11]杨英伟,刘恩顺.急性肺损伤/急性呼吸窘迫综合征中医病因病机及辨证分型的研究进展[J].辽宁中医杂志,2013,56(9):1937-1939.
[12]刘恩顺,孙增涛,苏景深,等.急性肺损伤/急性呼吸窘迫综合征肺肠相关证候特征及其演变的临床调查[J].中华中医药杂志,2013,28(10):3125-3127.