Lgr5、Arl4c在结肠癌组织中的表达及其对肿瘤恶性生物学行为的影响
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摘要
目的探讨Lgr5、Arl4c在结肠癌组织中的表达及其对肿瘤恶性生物学行为的影响。方法收集2014年6月-2017年1月在本院确诊并接受治疗的原发性结肠癌患者80例,肠镜下取结肠癌组织、癌旁组织,采用荧光定量PCR法检测其中Lgr5、Arl4c表达量,并根据肿瘤组织中Lgr5、Arl4c表达量中位数分为高Lgr5基因表达组、低Lgr5表达组,高Arl4c表达组、低Arl4c表达组各40例。对比不同Lgr5、Arl4c表达的肿瘤组织中增殖、侵袭基因表达量的差异。结果结肠癌组织中Lgr5、Arl4c mRNA表达量均显著高于癌旁组织(P<0.05);高Lgr5表达组增殖基因MS4A12 mRNA的表达量低于低Lgr5表达组,h TERT、Sph K1 mRNA的表达量高于低Lgr5表达组,侵袭基因LIMK1、PRDX1、PTTG1mRNA的表达量高于低Lgr5表达组,ZNRD1 mRNA表达量低于低Lgr5表达组(P<0.05);高Arl4c表达组增殖基因MS4A12 mRNA的表达量低于低Arl4c表达组,h TERT、Sph K1 mRNA的表达量高于低Arl4c表达组,侵袭基因LIMK1、PRDX1、PTTG1 mRNA的表达量高于低Arl4c表达组,ZNRD1 mRNA表达量低于低Arl4c表达组(P<0.05)。结论结肠癌组织中存在Lgr5、Arl4c高表达,可直接增加肿瘤细胞的增殖、侵袭活性。
Objective To study the expressions of Lgr5 and Arl4 c in colon cancer tissue and their influence on malignant biological behavior. Methods 80 cases of colon cancer patients in our hospital during June 2014 to January 2017 were chosen as research subjects,colon cancer tissue and paracancerous tissue were token and the expressions of Lgr5,Arl4 c gene were measured by fluorescent quantitative PCR. According to the expression of Lgr5 and Arl4 c gene in tumor tissue,all patients were divided into high Lgr5 expression group,low Lgr5 expression group,high Arl4 c expression group,low Arl4 c expression group,with 40 cases in each group. The differences of proliferation and invasion gene expression were compared. Results Expression of Lgr5,Arl4 c mRNA in colon cancer tissue were higher than those in paracancerous tissue( P < 0. 05); expression of MS4 A12 mRNA in high Lgr5 expression group was lower than that in low Lgr5 expression group,expressions of h TERT,Sph K1 mRNA were higher than those in low Lgr5 expression group,expressions of LIMK1,PRDX1,PTTG1 mRNA were higher than those in low Lgr5 expression group,ZNRD1 mRNA was lower than that in low Lgr5 expression group( P < 0. 05); expression of MS4 A12 mRNA in high Arl4 c expression group was lower than that in low Arl4 c expression group,expressions of h TERT and Sph K1 mRNA were higher than those in low Arl4 c expression group,LIMK1,PRDX1,PTTG1 mRNA were higher than those in low Arl4 c expression group,ZNRD1 mRNA was lower than that in low Arl4 c expression group( P < 0. 05). Conclusion There are high expressions of Lgr5 and Arl4 c in colon cancer tissue,which can directly increase the proliferation and invasion of tumor cells.
Objective To study the expressions of Lgr5 and Arl4 c in colon cancer tissue and their influence on malignant biological behavior. Methods 80 cases of colon cancer patients in our hospital during June 2014 to January 2017 were chosen as research subjects,colon cancer tissue and paracancerous tissue were token and the expressions of Lgr5,Arl4 c gene were measured by fluorescent quantitative PCR. According to the expression of Lgr5 and Arl4 c gene in tumor tissue,all patients were divided into high Lgr5 expression group,low Lgr5 expression group,high Arl4 c expression group,low Arl4 c expression group,with 40 cases in each group. The differences of proliferation and invasion gene expression were compared. Results Expression of Lgr5,Arl4 c mRNA in colon cancer tissue were higher than those in paracancerous tissue( P < 0. 05); expression of MS4 A12 mRNA in high Lgr5 expression group was lower than that in low Lgr5 expression group,expressions of h TERT,Sph K1 mRNA were higher than those in low Lgr5 expression group,expressions of LIMK1,PRDX1,PTTG1 mRNA were higher than those in low Lgr5 expression group,ZNRD1 mRNA was lower than that in low Lgr5 expression group( P < 0. 05); expression of MS4 A12 mRNA in high Arl4 c expression group was lower than that in low Arl4 c expression group,expressions of h TERT and Sph K1 mRNA were higher than those in low Arl4 c expression group,LIMK1,PRDX1,PTTG1 mRNA were higher than those in low Arl4 c expression group,ZNRD1 mRNA was lower than that in low Arl4 c expression group( P < 0. 05). Conclusion There are high expressions of Lgr5 and Arl4 c in colon cancer tissue,which can directly increase the proliferation and invasion of tumor cells.
引文
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[15]姚宇宙,易文,罗云春,等.PTTG1促进结肠癌SW480细胞侵袭和迁移的作用机制[J].肿瘤防治研究,2016,43(11):948-952.
[2]Chen P,Zhang JY,Sha BB,et al.Luteolin inhibits cell proliferation and induces cell apoptosis via down-regulation of mitochondrial membrane potential in esophageal carcinoma cells EC1 and KYSE450[J].Oncotarget,2017,8(16):27471-27480.
[3]舒新军.Lgr5在结肠癌组织中的表达及促进转移的临床研究[J].海南医学院学报,2016,22(9):833-836.
[4]訾永宏,姬乐,陈红梅,等.Arl4c在结肠癌中的表达及其临床意义[J].山西医科大学学报,2017,48(1):44-47.
[5]Gautam AK,Wang C,Zeng J,et al.Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer[J].Oncol Lett,2015,10(6):3629-3634.
[6]Shen WW,Zeng Z,Zhu WX,et al.MiR-142-3p functions as a tumor suppressor by targeting CD133,ABCG2,and Lgr5 in colon cancer cells[J].J Mol Med(Berl),2013,91(8):989-1000.
[7]Chen X,Su Z,Wang S,et al.Clinical and prognostic significance of Arl4c expression in colorectal cancer[J].Cancer Biomark,2016,16(2):253-257.
[8]Jin Z,Yan W,Jin H,et al.Psoralidin inhibits proliferation and enhances apoptosis of human esophageal carcinoma cells via NF-κB and PI3K/Akt signaling pathways[J].Oncol Lett,2016,12(2):971-976.
[9]贺理,周冰倩,宋淑佳,等.MS4A12基因在结肠癌中的表达及对结肠癌细胞增殖的影响[J].基因组学与应用生物学,2016,35(9):2217-2220.
[10]肖琳,王平,董俊红,等.RNAi双沉默Survivin和h TERT基因抑制人结肠癌SW480细胞增殖促进凋亡[J].基础医学与临床,2015,35(1):38-41.
[11]苏颖洁,黄杰安,刘诗权,等.Sph K1对结肠癌lovo细胞的增殖、迁移和凋亡的影响[J].世界华人消化杂志,2012,20(4):276-281.
[12]Liu X,Wang Z,Zhang G,et al.High TRAF6 expression is associated with esophageal carcinoma recurrence and prompts cancer cell invasion[J].Oncol Res,2017,25(4):485-493.
[13]Tantai JC,Zhang Y,Zhao H.Heterophyllin B inhibits the adhesion and invasion of ECA-109 human esophageal carcinoma cells by targeting PI3K/AKT/β-catenin signaling[J].Mol Med Rep,2016,13(2):1097-1104.
[14]冯继红,冯冬冬,傅仲学.PRDX1通过上皮间质转化促进结肠癌SW480细胞侵袭转移[J].遵义医学院学报,2015,38(1):67-70.
[15]姚宇宙,易文,罗云春,等.PTTG1促进结肠癌SW480细胞侵袭和迁移的作用机制[J].肿瘤防治研究,2016,43(11):948-952.