反胶束体系中脂肪酶催化合成单月桂酸甘油酯
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摘要
研究了在二-(2-乙基己基)琥珀酸酯磺酸钠(AOT)/异辛烷反胶束体系中,奶酪香精脂肪酶催化三月桂酸甘油酯和甘油反应制备单月桂酸甘油酯,并研究了反胶束体系的基本特性。结果表明:随着AOT质量浓度的增加,反胶束体系的增溶水量(W_0)、"水池"直径及黏度增加;最佳工艺条件为反应温度55℃、振荡器转速180 r/min、AOT质量浓度0. 1 g/m L、含水量0. 05 m L/m L、脂肪酶用量50 mg/m L、缓冲液pH 7. 5、底物摩尔比(甘油与三月桂酸甘油酯摩尔比) 3∶1、反应时间10h,此时体系中单月桂酸甘油酯的质量分数为84. 4%;当AOT质量浓度和含水量较小时,反胶束体系的W_0增加可促进酶催化反应的进行,但是当W_0和体系相对黏度继续增加,单月桂酸甘油酯的质量分数反而降低。
Palatase 20000 L was used in bis( 2-ethylhexyl) sulfosuccinate( AOT)/isooctane reversed micellar system to produce monolaurin from trilaurin and glycerol,and the basic properties of reversed micellar system were explored. The results showed that with the increase of AOT mass concentration,the volume of water which could be solubilized in the system increased,as well as the viscosity and the diameter of ‘water pool'. The optimal parameters were obtained as follows: reaction temperature 55 ℃,stirring rate 180 r/min,AOT mass concentration 0. 1 g/mL,water content 0. 05 mL/mL,lipase dosage 50 mg/mL,buffer pH 7. 5,molar ratio of glycerol to trilaurin 3∶ 1 and reaction time 10 h. Under these conditions,the mass fraction of monolaurin in the reaction system was 84. 4%. When the AOT mass concentration and the water content were lower,the enzymatic reaction could be promoted with the increase of W_0 of the reversed micellar system. However,the mass fraction of monolaurin decreased when the W_0 and relative viscosity continued to increase.
Palatase 20000 L was used in bis( 2-ethylhexyl) sulfosuccinate( AOT)/isooctane reversed micellar system to produce monolaurin from trilaurin and glycerol,and the basic properties of reversed micellar system were explored. The results showed that with the increase of AOT mass concentration,the volume of water which could be solubilized in the system increased,as well as the viscosity and the diameter of ‘water pool'. The optimal parameters were obtained as follows: reaction temperature 55 ℃,stirring rate 180 r/min,AOT mass concentration 0. 1 g/mL,water content 0. 05 mL/mL,lipase dosage 50 mg/mL,buffer pH 7. 5,molar ratio of glycerol to trilaurin 3∶ 1 and reaction time 10 h. Under these conditions,the mass fraction of monolaurin in the reaction system was 84. 4%. When the AOT mass concentration and the water content were lower,the enzymatic reaction could be promoted with the increase of W_0 of the reversed micellar system. However,the mass fraction of monolaurin decreased when the W_0 and relative viscosity continued to increase.
引文
[1]LOPES L Q,SANTOS C G,VAUCHER R A,et al.Nanocapsules with glycerol monolaurate:effects on Candida albicans biofilms[J].Microb Pathogen,2016,97:119-124.
[2]张震,曹茜,汪勇,等.固体超强酸催化大豆油和大豆油脂肪酸酯化与酯交换制备单甘酯的研究[J].中国油脂,2013,38(10):48-51.
[3]ZHENG H X,DENG L L,QUE F,et al.Physical characterization and antimicrobial evaluation of glycerol monolaurate organogels[J].Colloid Surface A,2016,502:19-25.
[4]李胜,马传国,王英丹,等.分子蒸馏单甘酯与谷维素-谷甾醇复合葵花籽油凝胶的制备[J].中国油脂,2017,42(4):32-36.
[5]MANGAS-SNCHEA J,SERRANO-ARNALDOS M,ADLERCREUTZ P.Effective and highly selective lipasemediated synthesis of 2-monoolein and 1,2-diolein in a two-phase system[J].J Mol Catal B:Enzym,2015,112:9-14.
[6]YANG T,REBSDORF M,ENGELRUD U,et al.Enzymatic production of monoacylglycerols containing polyunsaturated fatty acids through an efficient glycerolysis system[J].J Agric Food Chem,2005,53(5):1475-1481.
[7]查宝萍,郑联合,王莉,等.固定化脂肪酶催化椰子油制备月桂酸单甘油酯[J].中国油脂,2013,38(11):64-67.
[8]LIANG Y S,YUAN X Z,ZENG G M,et al.Effects of the surfactants on enzyme-containing reversed micellar system[J].Sci China Chem,2011,54(5):763-772.
[9]吕松泰,邹孝强,钱海峰,等.离子液体中酶法酯化选择性富集单甘酯的研究[J].中国油脂,2017,42(8):37-43.
[10]ITABAIANA I,Jr,GONCALVESS K M,CORDEIRO YM L,et al.Kinetics and mechanism of lipase catalyzed monoacylglycerols synthesis[J].J Mol Catal B:Enzym,2013,96:34-39.
[11]PARK K M,KWON O T,AHN S M,et al.Characterization and optimization of carboxylesterase-catalyzed esterification between capric acid and glycerol for the production of 1-monocaprin in reversed micellar system[J].New Biotechnol,2009,27(1):46-52.
[12]KINUGASA T,KONDO A,NISHIMURA S,et al.Estimation for size of reverse micelles formed by AOT and SDEHP based on viscosity measurement[J].Colloid Surface A,2002,204(1):193-199.
[13]赵晓燕,陈复生,薛文通.不同反胶束体系提取大豆蛋白质的研究[J].食品科学,2007,28(4):109-112.
[14]马洪超,袁杰,于丽,等.水相和特殊介质中有序聚集体的结构、性质和应用(VI)---反相胶束/微乳液、反相溶致液晶和囊泡[J].日用化学工业,2010,40(2):129-140.
[15]HEMAVATHI A B,HEBBAR H U,RAGHAVARAO K SM S.Mixed reverse micellar systems for extraction and purification ofβ-glucosidase[J].Sep Purif Technol,2010,71(2):263-268.
[16]曾家豫,唐功,周兴辉,等.非水相中脂肪酶催化合成乙酸薄荷酯[J].食品工业科技,2008,29(11):232-234,247.
[17]KOYAMATSU Y,HIRANO T,KAKIZAWA Y,et al.p H-responsive release of proteins from biocompatible and biodegradable reverse polymer micelles[J].J Control Release,2013,173(1):89-95.
[18]胡隼,万鹏,王芳,等.脂肪酶催化大豆色拉油甘油解合成单甘酯[J].中国粮油学报,2007,22(3):80-84.
[19]NAIK M K,NAIK S N,MOHANTY S.Enzymatic glycerolysis for conversion of sunflower oil to food based emulsifiers[J].Catal Today,2014,237(6):145-149.
[20]BADENES S M,LEMOS F,CABRAL J M S.Transesterification of oil mixtures catalyzed by microencapsulated cutinase in reversed micelles[J].Biotechnol Lett,2010,32(3):399-403.
[21]刘伟东,聂开立,鲁吉珂,等.反胶束体系中脂肪酶催化合成生物柴油[J].生物工程学报,2008,24(1):142-146.
[22]BIASUTTI M A,ABUIN E B,SILBER J J,et al.Kinetics of reactions catalyzed by enzymes in solutions of surfactants[J].Adv Colloid Interface Sci,2008,136(1/2):1-24.
[2]张震,曹茜,汪勇,等.固体超强酸催化大豆油和大豆油脂肪酸酯化与酯交换制备单甘酯的研究[J].中国油脂,2013,38(10):48-51.
[3]ZHENG H X,DENG L L,QUE F,et al.Physical characterization and antimicrobial evaluation of glycerol monolaurate organogels[J].Colloid Surface A,2016,502:19-25.
[4]李胜,马传国,王英丹,等.分子蒸馏单甘酯与谷维素-谷甾醇复合葵花籽油凝胶的制备[J].中国油脂,2017,42(4):32-36.
[5]MANGAS-SNCHEA J,SERRANO-ARNALDOS M,ADLERCREUTZ P.Effective and highly selective lipasemediated synthesis of 2-monoolein and 1,2-diolein in a two-phase system[J].J Mol Catal B:Enzym,2015,112:9-14.
[6]YANG T,REBSDORF M,ENGELRUD U,et al.Enzymatic production of monoacylglycerols containing polyunsaturated fatty acids through an efficient glycerolysis system[J].J Agric Food Chem,2005,53(5):1475-1481.
[7]查宝萍,郑联合,王莉,等.固定化脂肪酶催化椰子油制备月桂酸单甘油酯[J].中国油脂,2013,38(11):64-67.
[8]LIANG Y S,YUAN X Z,ZENG G M,et al.Effects of the surfactants on enzyme-containing reversed micellar system[J].Sci China Chem,2011,54(5):763-772.
[9]吕松泰,邹孝强,钱海峰,等.离子液体中酶法酯化选择性富集单甘酯的研究[J].中国油脂,2017,42(8):37-43.
[10]ITABAIANA I,Jr,GONCALVESS K M,CORDEIRO YM L,et al.Kinetics and mechanism of lipase catalyzed monoacylglycerols synthesis[J].J Mol Catal B:Enzym,2013,96:34-39.
[11]PARK K M,KWON O T,AHN S M,et al.Characterization and optimization of carboxylesterase-catalyzed esterification between capric acid and glycerol for the production of 1-monocaprin in reversed micellar system[J].New Biotechnol,2009,27(1):46-52.
[12]KINUGASA T,KONDO A,NISHIMURA S,et al.Estimation for size of reverse micelles formed by AOT and SDEHP based on viscosity measurement[J].Colloid Surface A,2002,204(1):193-199.
[13]赵晓燕,陈复生,薛文通.不同反胶束体系提取大豆蛋白质的研究[J].食品科学,2007,28(4):109-112.
[14]马洪超,袁杰,于丽,等.水相和特殊介质中有序聚集体的结构、性质和应用(VI)---反相胶束/微乳液、反相溶致液晶和囊泡[J].日用化学工业,2010,40(2):129-140.
[15]HEMAVATHI A B,HEBBAR H U,RAGHAVARAO K SM S.Mixed reverse micellar systems for extraction and purification ofβ-glucosidase[J].Sep Purif Technol,2010,71(2):263-268.
[16]曾家豫,唐功,周兴辉,等.非水相中脂肪酶催化合成乙酸薄荷酯[J].食品工业科技,2008,29(11):232-234,247.
[17]KOYAMATSU Y,HIRANO T,KAKIZAWA Y,et al.p H-responsive release of proteins from biocompatible and biodegradable reverse polymer micelles[J].J Control Release,2013,173(1):89-95.
[18]胡隼,万鹏,王芳,等.脂肪酶催化大豆色拉油甘油解合成单甘酯[J].中国粮油学报,2007,22(3):80-84.
[19]NAIK M K,NAIK S N,MOHANTY S.Enzymatic glycerolysis for conversion of sunflower oil to food based emulsifiers[J].Catal Today,2014,237(6):145-149.
[20]BADENES S M,LEMOS F,CABRAL J M S.Transesterification of oil mixtures catalyzed by microencapsulated cutinase in reversed micelles[J].Biotechnol Lett,2010,32(3):399-403.
[21]刘伟东,聂开立,鲁吉珂,等.反胶束体系中脂肪酶催化合成生物柴油[J].生物工程学报,2008,24(1):142-146.
[22]BIASUTTI M A,ABUIN E B,SILBER J J,et al.Kinetics of reactions catalyzed by enzymes in solutions of surfactants[J].Adv Colloid Interface Sci,2008,136(1/2):1-24.