ASBR系统中磷化氢浓度与碱性磷酸酶活性的关系
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摘要
以厌氧序批式反应器(ASBR)为研究对象,通过对反应器进水和出水中TP、COD和沼气中自由态磷化氢的长期监测,确定体系存在稳定的磷化氢产出。在此基础上,研究了系统在典型周期内TP、COD、磷化氢和碱性磷酸酶的变化规律,分析了磷化氢和碱性磷酸酶之间的相互关系。结果表明:TP、COD在反应过程中呈下降趋势且在5 h时实现最大去除,随后上下小幅波动;气态磷化氢(PH3)变化趋势呈"S"形;结合态磷化氢(MBP)和碱性磷酸酶活性(APA)变化趋势一致,均于3 h时骤升、4 h陡降、7 h小幅上升,然后波动;PH_3与APA没有明显的相关关系,MBP与APA呈显著正相关(R~2=0.964,P<0.01)。
The production of phosphine in anaerobic sequencing batch reactor( ASBR) was studied. After the long-term monitoring of total phosphorus( TP),chemical oxygen demand( COD),and phosphine,it was determined that the system had a stable production of phosphine. The changes in TP,COD,phosphine,and alkaline phosphatase in the typical cycle were studied,and the relationship between phosphine and alkaline phosphatase was analyzed. TP and COD showed a decreasing trend in the reaction process,reached a maximum removal at 5 h,and then fluctuated slightly. The change of gaseous phosphine( PH_3) showed an"S"shape. The matrix-bound phosphine( MBP) and alkaline phosphatase activity( APA) both rose dramatically at 3 h,dropped sharply at 4 h,and then increased moderately at7 h. No significant correlation was identified between PH3 concentrations and APA; while,MBP concentrations showed a significant positive correlation with APA( R~2= 0. 964,P < 0. 01).
The production of phosphine in anaerobic sequencing batch reactor( ASBR) was studied. After the long-term monitoring of total phosphorus( TP),chemical oxygen demand( COD),and phosphine,it was determined that the system had a stable production of phosphine. The changes in TP,COD,phosphine,and alkaline phosphatase in the typical cycle were studied,and the relationship between phosphine and alkaline phosphatase was analyzed. TP and COD showed a decreasing trend in the reaction process,reached a maximum removal at 5 h,and then fluctuated slightly. The change of gaseous phosphine( PH_3) showed an"S"shape. The matrix-bound phosphine( MBP) and alkaline phosphatase activity( APA) both rose dramatically at 3 h,dropped sharply at 4 h,and then increased moderately at7 h. No significant correlation was identified between PH3 concentrations and APA; while,MBP concentrations showed a significant positive correlation with APA( R~2= 0. 964,P < 0. 01).
引文
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[11]姜经梅,赵慧,沈铭能,等.长江口潮滩表层沉积物中碱性磷酸酶活性及其影响因素[J].环境科学学报,2011,31(10):2233-2239.Jiang Jingmei,Zhao Hui,Shen Mingneng,et al.Distribution and impact factor of alkaline phosphatase activity in the intertidal surface sediments of the Yangtze Estuary[J].Acta Scientiae Circumstantiae,2011,31(10):2233-2239(in Chinese).
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[13]游丽丽,宗海波,张淑芳,等.金普湾海域表层沉积物中基质结合态磷化氢的分布特征[J].环境科学,2013,34(10):3804-3809.You Lili,Zong Haibo,Zhang Shufang,et al.Distribution of matrix-bound phosphine in surface sediments of Jinpu Bay[J].Environmental Science,2013,34(10):3804-3809(in Chinese).
[14]罗秋容.厌氧除磷过程的影响因素和功能菌的筛选[J].能源与节能,2013,(4):64-66.Luo Qiurong.The factor of anaerobic dephosphorization process and the screening of bacteria[J].Energy and Energy Conservation,2013,(4):64-66(in Chinese).
[15]周康群,刘晖,孙彦富,等.厌氧条件总磷还原为磷化氢种泥筛选及功能菌株鉴定[J].生态环境,2007,16(6):1669-1673.Zhou Kangqun,Liu Hui,Sun Yanfu,et al.Screening the phosphate reducer deoxidizing total phosphate into PH3and the identification of the functional bacteria[J].Ecology and Environment,2007,16(6):1669-1673(in Chinese).
[16]周康群,刘晖,孙彦富,等.磷酸盐还原为磷化氢功能菌株的筛选与鉴定[J].农业环境科学学报,2007,26(4):1512-1516.Zhou Kangqun,Liu Hui,Sun Yanfu,et al.Screening and identification of deoxidizing phosphate into PH3bacterium strains[J].Journal of Agro-Environment Science,2007,26(4):1512-1516(in Chinese).
[17]Xie C,Zhao J,Tang J,et al.The phosphorus fractions and alkaline phosphatase activities in sludge[J].Bioresour Technol,2011,102(3):2455-2461.
[18]Zhu R,Ma D,Ding W,et al.Occurrence of matrixbound phosphine in polar ornithogenic tundra ecosystems:Effects of alkaline phosphatase activity and environmental variables[J].Science of The Total Environment,2011,409(19):3789-3800.
[19]牛晓君.沉积物中磷化氢产生的微生物作用初步探讨[J].中国科技论文在线,2010,5(5):373-376.Niu Xiaojun.Preliminary research on phosphine production by microorganisms in sediments[J].Sciencepaper Online,2010,5(5):373-376(in Chinese).
[20]王瑾丰,牛晓君,马金玲,等.厌氧条件下微生物将磷还原为磷化氢[J].微生物学通报,2015,42(1):34-41.Wang Jinfeng,Niu Xiaojun,Ma Jinling,et al.Conversion of phosphorus to phosphine by microbial deoxidization under anaerobic conditions[J].Microbiology China,2015,42(1):34-41(in Chinese).
[21]李怀.ASBR处理水热改性污泥的微生物学特性研究[D].西安:长安大学,2008.Li Huai.A Study on Microbiology of ASBR Reactor Treating Thermal Hydrolysis Sewage Sludge[D].Xi’an:Chang’an University,2008(in Chinese).
[22]Hou L,Liu M,Ding P,et al.Influences of sediment dessication on phosphorus transformations in an intertidal marsh:Formation and release of phosphine[J].Chemosphere,2011,83(7):917-924.
[23]Hong Y,Geng J,Qiao S,et al.Distribution of phosphine in the offshore area of the Southwest Yellow Sea,East Asia[J].Marine Chemistry,2010,118(1/2):67-74.
[24]陈垚,周健,甘春娟,等.初始p H值对磷酸盐还原除磷的影响研究[J].环境工程学报,2011,5(11):2428-2432.Chen Yao,Zhou Jian,Gan Chunjuan,et al.Effect of initial p H value on phosphorus removal of phosphate reduction[J].Chinese Journal of Environmental Engineering,2011,5(11):2428-2432(in Chinese).
[25]周健,粟静静,窦艳艳,等.无污泥外排除磷系统的磷平衡及除磷途径分析[J].华南理工大学学报:自然科学版,2011,39(6):95-99.Zhou Jian,Su Jingjing,Dou Yanyan,et al.Analyses of phosphorus balance and phosphorus removal way of phosphorus removal system without sludge discharge[J].Journal of South China University of Technology:Natural Science Edition,2011,39(6):95-99(in Chinese).
[2]Yang S,Yang F,Fu Z,et al.Simultaneous nitrogen and phosphorus removal by a novel sequencing batch moving bed membrane bioreactor for wastewater treatment[J].J Hazard Mater,2010,175(1/3):551-557.
[3]Hou L J,Liu M,Zhou J L,et al.Emission of phosphine in intertidal marshes of the Yangtze Estuary[J].Appl Geochem,2011,26(12):2260-2265.
[4]孟顺龙,裘丽萍,陈家长,等.污水化学沉淀法除磷研究进展[J].中国农学通报,2012,28(35):264-268.Meng Shunlong,Qiu Liping,Chen Jiazhang,et al.The research process of chemistry precipitation method in phosphorus removal in wastewater[J].Chinese Agricultural Science Bulletin,2012,28(35):264-268(in Chinese).
[5]杨帆,王冬波,李小明,等.单级好氧除磷工艺与厌氧/好氧除磷工艺的对比研究[J].环境科学,2011,32(11):3379-3385.Yang Fan,Wang Dongbo,Li Xiaoming,et al.Comparison study on phosphorus removal between single-stage oxic process and anaerobic/aerobic process[J].Environmental Science,2011,32(11):3379-3385(in Chinese).
[6]张可方,张朝升,韦伟.好氧及厌氧/好氧序批式工艺中磷化氢的释放[J].环境工程,2011,29(5):127-134.Zhang Kefang,Zhang Chaosheng,Wei Wei.Phosphine release in aerobic sequencing reactor process and anaerobic/aerobic sequencing reactor process[J].Environmental Engineering,2011,29(5):127-134(in Chinese).
[7]孙亮.磷化氢的释放机理及其过程研究[D].广州:广州大学,2012.Sun Liang.Study on Mechanism and Process of the Phosphine Released[D].Guangzhou:Guangzhou University,2012(in Chinese).
[8]张楠.谈在厌氧好氧条件下磷的气体转化形式[J].山西建筑,2012,38(22):134-135.Zhang Nan.Gaseous transform shape of phosphorus under anaerobic-aerobic condition[J].Shanxi Architecture,2012,38(22):134-135(in Chinese).
[9]罗野,鲍建国,李书敏,等.磷化氢的释放对提高生活污水除磷效果的试验研究[J].安全与环境工程,2014,21(5):94-97.Luo Ye,Bao Jianguo,Li Shumin,et al.Experimental study on the release of phosphine to improve the removal effect of phosphorus from sewage[J].Safety and Environmental Engineering,2014,21(5):94-97(in Chinese).
[10]刘志培,王宝军,贾省芬,等.样品磷化氢含量与某些微生物群落及酶活的关系[J].微生物学报,2006,46(4):608-612.Liu Zhipei,Wang Baojun,Jia Shengfen,et al.Relationships between phosphine content of samples and their microbial populations and enzyme activities[J].Acta Microbiologica Sinica,2006,46(4):608-612(in Chinese).
[11]姜经梅,赵慧,沈铭能,等.长江口潮滩表层沉积物中碱性磷酸酶活性及其影响因素[J].环境科学学报,2011,31(10):2233-2239.Jiang Jingmei,Zhao Hui,Shen Mingneng,et al.Distribution and impact factor of alkaline phosphatase activity in the intertidal surface sediments of the Yangtze Estuary[J].Acta Scientiae Circumstantiae,2011,31(10):2233-2239(in Chinese).
[12]李涛,侯立军,刘敏,等.长江口近岸表层沉积物中基质结合态磷化氢的分布特征[J].环境科学,2012,33(10):3443-3448.Li Tao,Hou Lijun,Liu Min,et al.Distributions of matrix-bound phosphine in surface sediments of the Yangtze Estuary[J].Environmental Science,2012,33(10):3443-3448(in Chinese).
[13]游丽丽,宗海波,张淑芳,等.金普湾海域表层沉积物中基质结合态磷化氢的分布特征[J].环境科学,2013,34(10):3804-3809.You Lili,Zong Haibo,Zhang Shufang,et al.Distribution of matrix-bound phosphine in surface sediments of Jinpu Bay[J].Environmental Science,2013,34(10):3804-3809(in Chinese).
[14]罗秋容.厌氧除磷过程的影响因素和功能菌的筛选[J].能源与节能,2013,(4):64-66.Luo Qiurong.The factor of anaerobic dephosphorization process and the screening of bacteria[J].Energy and Energy Conservation,2013,(4):64-66(in Chinese).
[15]周康群,刘晖,孙彦富,等.厌氧条件总磷还原为磷化氢种泥筛选及功能菌株鉴定[J].生态环境,2007,16(6):1669-1673.Zhou Kangqun,Liu Hui,Sun Yanfu,et al.Screening the phosphate reducer deoxidizing total phosphate into PH3and the identification of the functional bacteria[J].Ecology and Environment,2007,16(6):1669-1673(in Chinese).
[16]周康群,刘晖,孙彦富,等.磷酸盐还原为磷化氢功能菌株的筛选与鉴定[J].农业环境科学学报,2007,26(4):1512-1516.Zhou Kangqun,Liu Hui,Sun Yanfu,et al.Screening and identification of deoxidizing phosphate into PH3bacterium strains[J].Journal of Agro-Environment Science,2007,26(4):1512-1516(in Chinese).
[17]Xie C,Zhao J,Tang J,et al.The phosphorus fractions and alkaline phosphatase activities in sludge[J].Bioresour Technol,2011,102(3):2455-2461.
[18]Zhu R,Ma D,Ding W,et al.Occurrence of matrixbound phosphine in polar ornithogenic tundra ecosystems:Effects of alkaline phosphatase activity and environmental variables[J].Science of The Total Environment,2011,409(19):3789-3800.
[19]牛晓君.沉积物中磷化氢产生的微生物作用初步探讨[J].中国科技论文在线,2010,5(5):373-376.Niu Xiaojun.Preliminary research on phosphine production by microorganisms in sediments[J].Sciencepaper Online,2010,5(5):373-376(in Chinese).
[20]王瑾丰,牛晓君,马金玲,等.厌氧条件下微生物将磷还原为磷化氢[J].微生物学通报,2015,42(1):34-41.Wang Jinfeng,Niu Xiaojun,Ma Jinling,et al.Conversion of phosphorus to phosphine by microbial deoxidization under anaerobic conditions[J].Microbiology China,2015,42(1):34-41(in Chinese).
[21]李怀.ASBR处理水热改性污泥的微生物学特性研究[D].西安:长安大学,2008.Li Huai.A Study on Microbiology of ASBR Reactor Treating Thermal Hydrolysis Sewage Sludge[D].Xi’an:Chang’an University,2008(in Chinese).
[22]Hou L,Liu M,Ding P,et al.Influences of sediment dessication on phosphorus transformations in an intertidal marsh:Formation and release of phosphine[J].Chemosphere,2011,83(7):917-924.
[23]Hong Y,Geng J,Qiao S,et al.Distribution of phosphine in the offshore area of the Southwest Yellow Sea,East Asia[J].Marine Chemistry,2010,118(1/2):67-74.
[24]陈垚,周健,甘春娟,等.初始p H值对磷酸盐还原除磷的影响研究[J].环境工程学报,2011,5(11):2428-2432.Chen Yao,Zhou Jian,Gan Chunjuan,et al.Effect of initial p H value on phosphorus removal of phosphate reduction[J].Chinese Journal of Environmental Engineering,2011,5(11):2428-2432(in Chinese).
[25]周健,粟静静,窦艳艳,等.无污泥外排除磷系统的磷平衡及除磷途径分析[J].华南理工大学学报:自然科学版,2011,39(6):95-99.Zhou Jian,Su Jingjing,Dou Yanyan,et al.Analyses of phosphorus balance and phosphorus removal way of phosphorus removal system without sludge discharge[J].Journal of South China University of Technology:Natural Science Edition,2011,39(6):95-99(in Chinese).