ATP生物发光法在喷气燃料微生物检测中的应用
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摘要
近年来,由于微生物污染带来的喷气燃料储存和使用问题日渐增多。喷气燃料中微生物的快速检测是有效治理微生物污染的前提。本文采用HY-LiTE~JET A1燃料试验对ATP生物发光法在喷气燃料微生物检测中的应用进行了研究。研究发现,该方法与国标平皿计数方法在一定范围内呈正相关(R~2=0.960 1),但也存在振荡标准不统一、萃取液萃取不完全、知识产权受限和耗材昂贵等诸多问题。最后展望了ATP生物发光法和滤膜集菌法未来的发展前景,对研制具有我国自主知识产权的喷气燃料微生物检测设备具有指导意义。
In recent years, the storage and usage problems of jet fuel caused by microbial contamination are increasing. Rapid detection of microorganisms in jet fuel is a prerequisite for the effective treatment of microorganisms. In this paper,HY-LiTE~ JET A1 fuel test was used to study the application of ATP bioluminescence method in the microbial detection of jet fuel. The results showed this method was positively correlated with the national standard of plate counting method(R~2=0.960 1) in a certain range, however there were many problems, such as inconsistent oscillation criteria, incomplete extraction, limited intellectual property rights and expensive materials. The prospects of ATP bioluminescence method and filter membrane method were briefly discussed, which has guiding significance for the development of microorganism detection equipment for jet fuel with independent intellectual property rights.
In recent years, the storage and usage problems of jet fuel caused by microbial contamination are increasing. Rapid detection of microorganisms in jet fuel is a prerequisite for the effective treatment of microorganisms. In this paper,HY-LiTE~ JET A1 fuel test was used to study the application of ATP bioluminescence method in the microbial detection of jet fuel. The results showed this method was positively correlated with the national standard of plate counting method(R~2=0.960 1) in a certain range, however there were many problems, such as inconsistent oscillation criteria, incomplete extraction, limited intellectual property rights and expensive materials. The prospects of ATP bioluminescence method and filter membrane method were briefly discussed, which has guiding significance for the development of microorganism detection equipment for jet fuel with independent intellectual property rights.
引文
[1]Mcelroy W D,Stremer B L.Factors influencing the Response of the Bioluminescent to Adenosine Triphosphate[J].Arch.Biochenl,1949,22:420-433.
[2]Marlene Deluca,William D.Mcelroy.Kinetics of the Firefly Lucif-erase Catalyzed Reactions[J].Biochemistry,1974,13(5):921-925.
[3]LaRossa R A.Methods in Molecular Biology,Vol 102:Bioluminescence Methods and protocols[M].Totowa,New Jersey:Humana press,1998:3-20.
[4]舒柏华,赵志耀,徐顺清,周宜开.利用生物发光分析技术快速检测微生物的方法学研究[J].发光学报,1999(01):79-82.
[5]Chappele E W,Levin G V.Method for radiorespirometric detection of bacteria in pure culture and in blood[J].Biochem Med,1968,2:41-52.
[6]James D M,Hendrson F.Nucleoside triphosphate speci-ficity of firely luciferase[J].Analytical Biochemistry,1983,19(11):194.
[7]Sevin BU,Peng Z I,Perras J P,et al.Application of an ATP bioluminescence assay in human tumor chemosensitivity testing[J].Gynecol oncol,1988,31(1):191-204.
[8]Sevin B U,Peng Z I,Averette H E,et al.Chemosensitivity testing in ovarian cancer[J].Cancer,1993,71(4):1613-1620.
[9]李鹏,熊云,陈然.喷气燃料中微生物污染危害研究概述[J].当代化工,2017,46(02):333-335+349.
[10]刘弋青,吕维敏,刘魁武,等.基于滤膜上细菌直接计数法的细菌总数快速检测[J].生物医学工程研究,2009,28(1):60-62.
[11]罗祎,赵晋府,周尔明.微生物检测国标法和滤膜法的比较[J].食品工业技,1999,20(6):54-56.
[12]嵇志远,翁天楚.三种方法测定水中粪便污染指示菌比较研究[J].工业水理,2013,33(10):79-82.
[13]Azadeh Namvar,Haq I,Shields M,er al.Extration of Bacillus endospores from water,apple juice concentrate,raw milk and lettuce rinse solutions using tangential flow filtration[J].Food Control,2013,32(2):632-637.
[14]Parveen S,Kaur S,David S A,et al.Wilson D,et al.Evaluation of growth based rapid microbiological methods for sterility testing of vaccines and other biological products[J].Vaccine,2011,29(45):8012-8023.
[2]Marlene Deluca,William D.Mcelroy.Kinetics of the Firefly Lucif-erase Catalyzed Reactions[J].Biochemistry,1974,13(5):921-925.
[3]LaRossa R A.Methods in Molecular Biology,Vol 102:Bioluminescence Methods and protocols[M].Totowa,New Jersey:Humana press,1998:3-20.
[4]舒柏华,赵志耀,徐顺清,周宜开.利用生物发光分析技术快速检测微生物的方法学研究[J].发光学报,1999(01):79-82.
[5]Chappele E W,Levin G V.Method for radiorespirometric detection of bacteria in pure culture and in blood[J].Biochem Med,1968,2:41-52.
[6]James D M,Hendrson F.Nucleoside triphosphate speci-ficity of firely luciferase[J].Analytical Biochemistry,1983,19(11):194.
[7]Sevin BU,Peng Z I,Perras J P,et al.Application of an ATP bioluminescence assay in human tumor chemosensitivity testing[J].Gynecol oncol,1988,31(1):191-204.
[8]Sevin B U,Peng Z I,Averette H E,et al.Chemosensitivity testing in ovarian cancer[J].Cancer,1993,71(4):1613-1620.
[9]李鹏,熊云,陈然.喷气燃料中微生物污染危害研究概述[J].当代化工,2017,46(02):333-335+349.
[10]刘弋青,吕维敏,刘魁武,等.基于滤膜上细菌直接计数法的细菌总数快速检测[J].生物医学工程研究,2009,28(1):60-62.
[11]罗祎,赵晋府,周尔明.微生物检测国标法和滤膜法的比较[J].食品工业技,1999,20(6):54-56.
[12]嵇志远,翁天楚.三种方法测定水中粪便污染指示菌比较研究[J].工业水理,2013,33(10):79-82.
[13]Azadeh Namvar,Haq I,Shields M,er al.Extration of Bacillus endospores from water,apple juice concentrate,raw milk and lettuce rinse solutions using tangential flow filtration[J].Food Control,2013,32(2):632-637.
[14]Parveen S,Kaur S,David S A,et al.Wilson D,et al.Evaluation of growth based rapid microbiological methods for sterility testing of vaccines and other biological products[J].Vaccine,2011,29(45):8012-8023.