乳化异氟醚静脉麻醉对大鼠中枢突触体ATP酶活性的影响
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摘要
为探讨乳化异氟醚对大鼠中枢突触体ATP酶活性的影响,选用36只Wistar大鼠随机分为对照组、麻醉组和麻醉恢复组,经乳化异氟醚尾静脉注射对大鼠进行麻醉,采用比色法检测各脑区的突触体ATP酶活性。结果显示:大鼠注射乳化异氟醚后大脑皮层、海马和丘脑Na+-K+-ATP酶和Ca2+-ATP酶与对照组相比显著下降(P<0.05),大脑皮质和海马Mg2+-ATP酶与对照组相比显著下降(P<0.05)。提示:乳化异氟醚能够抑制大脑皮层、海马和丘脑内Na+-K+-ATP酶和Ca2+-ATP酶的生成,抑制大脑皮质和海马内Mg2+-ATP酶的生成,推测中枢突触体ATP酶是乳化异氟醚产生麻醉效应的主要靶位。
To explore the effect of emulsified isoflurane on the central synaptosome ATPase activity in rat,36 Wistar rats were used and randomly divided into the control group,the anesthesia group and the anesthesia recovery group. The rats were anesthetized by emulsified isoflurane through intravenous injection in tail. The synaptosome ATPase activity in different regions were detected using the colorimetric method. The results showed that administration of emulsified isoflurane significantly(P<0. 05) reduced the activity of Na+-k+-ATPase and Ca~(2+)-ATPase in the cerebral cortex,hippocampus and hypothalamus of treated rats,compared with control group; and significantly(P <0. 05) decreased the activity of Mg~(2+)-ATPase in the cerebral cortex and hippocampus of rats,too. These findings indicate that emulsified isoflurane can inhibit the production of Na+-k+-ATPase and Ca~(2+)-ATPase in the cerebral cortex,hippocampus,and thalamus of rats,and also inhibit the production of Mg~(2+)-ATPase in the cerebral cortex and hippocampus of the rodents. Central synaptosome ATPase might be the important target at which emulsified isoflurane produced the analgesic effect.
To explore the effect of emulsified isoflurane on the central synaptosome ATPase activity in rat,36 Wistar rats were used and randomly divided into the control group,the anesthesia group and the anesthesia recovery group. The rats were anesthetized by emulsified isoflurane through intravenous injection in tail. The synaptosome ATPase activity in different regions were detected using the colorimetric method. The results showed that administration of emulsified isoflurane significantly(P<0. 05) reduced the activity of Na+-k+-ATPase and Ca~(2+)-ATPase in the cerebral cortex,hippocampus and hypothalamus of treated rats,compared with control group; and significantly(P <0. 05) decreased the activity of Mg~(2+)-ATPase in the cerebral cortex and hippocampus of rats,too. These findings indicate that emulsified isoflurane can inhibit the production of Na+-k+-ATPase and Ca~(2+)-ATPase in the cerebral cortex,hippocampus,and thalamus of rats,and also inhibit the production of Mg~(2+)-ATPase in the cerebral cortex and hippocampus of the rodents. Central synaptosome ATPase might be the important target at which emulsified isoflurane produced the analgesic effect.
引文
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[11]卢德章,范宏刚,王洪斌,等.小型猪特异性麻醉颉颃剂对大鼠不同脑区Ca2+,Mg2+-ATP酶活性的影响[J].畜牧与兽医,2013,45(1):53-55.
[12]尹柏双,王秋竹,付连军,等.鹿特异性复合麻醉剂对大鼠不同脑区ATP酶活性的影响[J].中国兽医学报,2014,34(11):1842-1845.
[13]郑宇,李德,潘佳亮,等.咪达唑仑对山羊不同脑区Ca2+-Mg2+-ATP酶活性的影响[J].中国兽医杂志,2016,52(7):16-18.
[2]马汉祥,李杰,杨宗斌,等.犬静脉注射乳化异氟醚与异丙酚麻醉的效果[J].中华麻醉学杂志,2006,26(9):862-863.
[3]张雷,顾尔伟,刘训芹,等.乳化异氟醚后处理对兔在体心脏缺血/再灌注损伤的保护作用[J].中国药理学通报,2009,25(10):1322-1325.
[4]段志祥,杨保仲.腹腔注射异氟醚、乳化异氟醚对小鼠肌肉松弛作用的观察[J].中国医疗前沿,2012,7(3):41-42.
[5]杨静,龚春雨,柴云飞,等.选择性外周神经给乳化异氟醚山羊模型的建立[J].四川大学学报,2010,41(2):332-336.
[6]Takashi K.Functional roles of ATP-sensitive potassium channel as related to anesthesia[J].J Anesth,2012,26(1):152-155.
[7]Shin W J,Winegar B D.Modulation of noninactivating K+channels in rat cerebellar granule neurons by halothane,isoflurane,and sevoflurane[J].Anesth Analg,2003,96(5):1340-1344.
[8]Rasmussen M K,Kristensen M,Juel C.Exercise-induced regulation of phospholemman(FXYD1)in rat skeletal muscle:implications for Na+/K+-ATPase activity[J].Acta Physiol,2008,194:67-79.
[9]Tokhtaeva E,Clifford R J,Kaplan J H,et al.Subunit isoform selectivity in assembly of Na,K-ATPaseα-βheterodimers[J].J Biol Chem,2012,287:26115-26125.
[10]Vedovato N,Gadsby D C.Route,mechanism,and implications of proton import during Na+/K+exchange by native Na+/K+-ATPase pumps[J].J Gen Physiol,2014,143:449-464.
[11]卢德章,范宏刚,王洪斌,等.小型猪特异性麻醉颉颃剂对大鼠不同脑区Ca2+,Mg2+-ATP酶活性的影响[J].畜牧与兽医,2013,45(1):53-55.
[12]尹柏双,王秋竹,付连军,等.鹿特异性复合麻醉剂对大鼠不同脑区ATP酶活性的影响[J].中国兽医学报,2014,34(11):1842-1845.
[13]郑宇,李德,潘佳亮,等.咪达唑仑对山羊不同脑区Ca2+-Mg2+-ATP酶活性的影响[J].中国兽医杂志,2016,52(7):16-18.