盐度对岩扇贝Na~+/K~+-ATP酶活性的影响
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摘要
岩扇贝为国内引进的新品种,为了弥补国内在盐度对岩扇贝影响这方面研究的空白,利用从加拿大引进的岩扇贝Crassadoma gigantea幼贝,经过在不同盐度梯度(高盐36,正常30,低盐24)及不同盐度变化速率驯化后,取样,选取幼贝内脏团,采用试剂盒检测方式,检测不同盐度梯度下岩扇贝Na~+/K~+-ATP酶活性的表达。结果表明:在盐度30下,Na~+/K~+-ATP酶活性维持在较高水平,而在高盐36胁迫下,酶活力较盐度30的低,且在盐度骤变情况下尤为显著;在低盐24胁迫下,酶活性同样较盐度30下的酶活力低,且相对高盐胁迫活性更低
Scallop is a new breed of shellfish imported from abroad.In order to make up for the study on the influence of salinity on scallop,Crassadoma gigantea young scallop imported from Canada is used to sample and select the young after different salinity gradient( high salt 36,normal 30,low salt 24) and different salinity change rates.The expression of Na+/K+-ATP enzyme activities of scallop in different tissues under different salinity gradient were detected by kit detection.The results show that: The activity of Na+/K+-ATPase was maintained at a high level at 30 salinity,but the activity of this enzymes was lower than that of salinity 30 under high salt 36 stress,especially in the case of sudden change of salinity.While in low salt 24 stress,the activity of these this enzymes was also lower than that under 30 salinity,and the activity of the two enzymes was lower than that of high salt stress.
Scallop is a new breed of shellfish imported from abroad.In order to make up for the study on the influence of salinity on scallop,Crassadoma gigantea young scallop imported from Canada is used to sample and select the young after different salinity gradient( high salt 36,normal 30,low salt 24) and different salinity change rates.The expression of Na+/K+-ATP enzyme activities of scallop in different tissues under different salinity gradient were detected by kit detection.The results show that: The activity of Na+/K+-ATPase was maintained at a high level at 30 salinity,but the activity of this enzymes was lower than that of salinity 30 under high salt 36 stress,especially in the case of sudden change of salinity.While in low salt 24 stress,the activity of these this enzymes was also lower than that under 30 salinity,and the activity of the two enzymes was lower than that of high salt stress.
引文
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[2]Phleger C F,Holtz RB,Grimes P W,et al.Chemical andsensoryanalysis of the purple-hinge rock scallo Hinnites multirugosusGale[J].Journal of Food Science,1978,43(6):1793-1796.
[3]Maxwell-Miller G,Josephson R V,Spindler A A,et al.Chilled(5℃)and frozen(-18℃)storage stability of the purple-hingerock scallop,Hinnites multirugosus Gale[J]. Journal of FoodScience,1982,47(5):1654-1661.
[4]Laurén D J.Oogenesis and protandry in the purple-hingerock scallop,Hinnites giganteus,in upper Puget Sound,Washington,U.S.A.[J].Canadian Journal of Zoology,1982,60(10):2333-2336.
[5]Cary S C,Leighton D L,Phleger C F. Food and feedingstrategies inculture of larval and early juvenile purplehinge rockscallops,Hinnites multirugosus(GALE)[J]. Journal of the WorldAquaculture Society,1981,12(1):156-169.
[6]Culver C S,Richards J B,Page H M. Plasticity ofattachment in the purple-hinge rock scallop,Crassadoma gigantea:implications forcommercial culture[J]. Aquaculture,2006,254(1-4):361-369.
[7]曹善茂,汪健,王谦,等.岩扇贝人工育苗的初步研究[J].大连海洋大学学报,2017,32(1):1-6.
[8]曹善茂,梁伟锋,汪健,等.岩扇贝幼贝滤食率的基础研究[J].大连海洋大学学报,2016,31(6):612-617.
[9]曹善茂,王昊,陈炜,等.岩扇贝闭壳肌营养成分的分析及与中国3种扇贝的比较[J].大连海洋大学学报,2016,31(5):544-550.
[10]肖克宇,陈昌福,李月红,等.水产动物免疫学[M].北京:中国农业出版社,2011:91-92.
[11]钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究.南方水产科学.[J].南方水产科学,2015.11(6):49-50.
[12]Wang W,Wang A,Liu Y,et al.Effect of temperature ongrowth,Adenosinephosphates,ATP ase and cellular defense responseof juvenile shrimp Macrobrachium nipponense[J]. Aquaculture,2006,256(1):624-630.
[13]Imsland A K,Gunnarsson S,Foss A,et al.Gill Na+,K+-ATPase activity,plasma chloride and osmolality in juvenile turbot(Scophthalmus maximus)reared at different temperatures and salin-ities.[J].Aquaculture,2003,218(1):671-683.
[14]张珂,洪美玲,史海涛,等.盐度胁迫对红耳龟Na+/K+-ATP酶及消化酶活性的影响[J].水产科学,2014,33(8):520-524.
[15]王茂林,李岑,杨敏,等.盐度对仿刺参存活和Na+/K+-ATP酶活性的影响[J].渔业现代化,2014,41(1):6-9.
[16]梁伟锋.不同因子对岩扇贝摄食率、耗氧率和排氨率的影响[D].大连海洋大学,2016.