苏尼特羊脂肪组织中脂肪酸沉积机制的研究
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摘要
本文以12月龄苏尼特羊不同脂肪组织(皮下脂肪、尾部脂肪、肾脏脂肪)为试验对象,利用气相色谱-质谱(GC-MS)技术和实时荧光定量PCR技术研究不同脂肪组织中脂肪酸含量和脂肪代谢相关基因mRNA表达量,同时分析脂肪代谢相关基因与脂肪酸间相关性。结果表明,苏尼特羊脂肪组织中脂肪酸以棕榈酸、硬脂酸和油酸为主。肾脂中硬脂酸的含量显著高于尾脂(p<0.05);尾脂中油酸含量显著高于皮脂和肾脂(p<0.05)。不同脂肪组织间,肾脂中硬脂酰辅酶A去饱和酶(SCD)、脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FASN)基因mRNA表达量显著高于皮脂和尾脂(p<0.05);皮脂中肉碱棕榈酰转移酶(CPT1)和乙酰辅酶A羧化酶(ACC)基因mRNA表达量显著高于肾脂(p<0.05)。通过相关性分析,共轭亚油酸与FADS1、LPL和SCD呈显著正相关(p<0.05);二十碳五烯酸(C_(20∶5))与FADS1和LPL间呈显著正相关(p<0.05),与ACC呈显著负相关(p<0.05)。由此可知,调控脂肪酸沉积相关基因的表达对脂肪酸沉积的方向和部位有明显的影响。
The different adipose tissue(subcutaneous fat,tail fat and kidney fat)of Sunit sheep with 12 months week age was used as the test object,and the gas chromatography-mass spectrometry(GC-MS)and real time polymerase chain reaction(RT-PCR)were used to research the contents of fatty acid and the mRNA expression of fat metabolism related gene,and the relationship between fat metabolism gene and fatty acid were also analyzed. The results showed that fatty acid in adipose tissue of Sunit sheep mainly included palmitic acid,stearic acid and oleic acid. The contents of stearic acid in kidney fat was significantly higher than that in tail fat(p<0.05),the ratio of oleic acid in tail fat was significantly higher than that in subcutaneous fat and kidney fat(p<0.05). The gene expression of FASN,SCD and LPL in kidney fat was significantly higher than that in subcutaneous fat and tail fat(p<0.05),the gene expression of CPT1 and ACC in subcutaneous fat was significantly higher than that in kidney fat(p<0.05). Correlation analysis showed that CLA was positively correlated with FADS1,LPL and SCD(p<0.05),C_(20∶5) was positively correlated with FADS1 and LPL(p<0.05)and negatively correlated with ACC(p<0.05). In conclusion,regulating the expression of genes related to fat deposition had a significant effect on the direction and location of fatty acid deposition.
The different adipose tissue(subcutaneous fat,tail fat and kidney fat)of Sunit sheep with 12 months week age was used as the test object,and the gas chromatography-mass spectrometry(GC-MS)and real time polymerase chain reaction(RT-PCR)were used to research the contents of fatty acid and the mRNA expression of fat metabolism related gene,and the relationship between fat metabolism gene and fatty acid were also analyzed. The results showed that fatty acid in adipose tissue of Sunit sheep mainly included palmitic acid,stearic acid and oleic acid. The contents of stearic acid in kidney fat was significantly higher than that in tail fat(p<0.05),the ratio of oleic acid in tail fat was significantly higher than that in subcutaneous fat and kidney fat(p<0.05). The gene expression of FASN,SCD and LPL in kidney fat was significantly higher than that in subcutaneous fat and tail fat(p<0.05),the gene expression of CPT1 and ACC in subcutaneous fat was significantly higher than that in kidney fat(p<0.05). Correlation analysis showed that CLA was positively correlated with FADS1,LPL and SCD(p<0.05),C_(20∶5) was positively correlated with FADS1 and LPL(p<0.05)and negatively correlated with ACC(p<0.05). In conclusion,regulating the expression of genes related to fat deposition had a significant effect on the direction and location of fatty acid deposition.
引文
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[28]梅秀丽.优质鸡不同饲养方式下FADS1、FADS2基因的表达及其对脂肪酸组成的影响[D].成都:四川农业大学,2012.
[29]曾瑛,唐蓓,李影. Micro RNAs在哺乳动物脂肪形成中的调控作用[J].中国细胞生物学学报,2015,37(1):1-9.
[30]Claudia G,Joachim H,Berthold K. Role of FADS1 and FADS2 polymorphisms in polyunsaturated fatty acid metabolism[J]. Metabolism,2016,59(7):993-996.
[31]Tananka T,Jian S,Abecasis GR,et al. Genome-wide association study of plasma polyunsaturated fatty acids in the InChianti Study[J]. Plos Genetics,2009,5(1):e1000338.
[32]Samulin J,Berget I,Grindflek E,et al. Changes in lipid metabolism associated gene transcripts during porcine adipogenesis[J]. Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology,2009,153(1):8-17.
[33]Madhu SR,Tyagi A,Hossai SA,et al. Effect of tanniniferous Terminalia chebula extract on rumen biohydrogenation,Δ9-desaturase activity,CLA content and fatty acid composition in longissimus dorsi muscle of kids[J].Meat Science,2012,90(3):558-563.
[4]李月娥,葛长荣. 羊肉膻味研究及脱膻技术的发展[J]. 肉品卫生,2005(12):27-29.
[2]Olga B,Olga G,Evgenya U,et al. The role of adipose tissue and adipokines in the manifestation of type 2 diabetes in the long-term period following myocardial infarction[J]. Diabetology and Metabolic Syndrome,2016,8(1):1-5.
[3]Liaw J J,Peplow P V. Differential effect of electroacupuncture on inflammatory adipokines in two rat models of obesity[J]. Journal of Acupunct Meridian Studies,2016,9(4):183-190.
[5]Samulin J,Berget I,Lien S,et al. Differential gene expression of fatty acid binding proteins during porcine adipogenesis[J]. Comparative biochemistry and Physiology. Part B,Biochemistry and Molecular Biology,2008,151(2):147-152.
[6]李娜.新疆褐牛四种脂代谢调控基因的表达及其与脂肪组织生物学的相关性研究[D].乌鲁木齐:新疆农业大学,2013.
[7]Aviles C,Horcada A,Polvillo O,et al. Association study between variability in the SCD gene and the fatty acid profile in perirenal and intramuscular fat deposits from Spanish goat populations[J]. Small Ruminant Research,2016,136:127-131.
[8]Taniguchi M,Mannen H,Oyama K,et al. Differences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle[J]. Livestock production Science,2004,87(2):215-220.
[9]戢爽.肉牛脂肪组织和背最长肌脂代谢相关基因差异表达分析[D].长春:吉林大学,2013.
[10]Folch L M,Sloane G H S. A simple method for isolation and purification of total lipids from animal tissues[J]. Journal of Biological Chemistry,1957,226(1):497-509.
[11]王乐.FoxO1与其调控肌纤维类型相关基因的研究及对羊肉质的影响[D].呼和浩特:内蒙古农业大学,2016.
[12]刚虎军,古扎力孜克·肉孜,苑贝贝,等. 南疆多浪羊不同部位脂肪组织中挥发性风味成分分析[J]. 肉类研究,2017,31(8):28-33.
[13]李维红,吴建平,王欣荣. 靖远滩羊体脂脂肪酸与肉品质关系的研究[J]. 中国草食动物科学,2005,25(1):54-55.
[14]Wood JD,Enser M,Fisher AV,et al. Fat deposition,fatty acid composition and meat quality:A review[J]. Meat Science,2008,78(4):343-358.
[15]杨东,王文义,乔文,等.肉羊脂肪沉积及其调控手段[J].粮食与饲料工业,2016(1):51-55.
[16]双金,敖力格日玛,敖长金.苏尼特羊体脂脂肪酸组成的研究[J].畜牧兽医学报,2015,46(8):1363-1374.
[17]孟影.延边黄牛不同部位脂肪组织脂肪酸构成及脂代谢基因的差异表达分析[D].延吉:延边大学,2017.
[18]Edwin N F,William E N,Edward S. Analysis of autoxidized fats by gas chromatography-mass spectrometry:VII. Volatile thermal decomposition products of pure hydroperoxides from autoxidized and photosensitized oxidized methyl oleate,linoleate and linolenate[J]. LIPIDS,1981,16(5):279-285.
[19]王柏辉,靳志敏,刘夏炜,等.影响羊肉中不饱和脂肪酸沉积因素的研究进展[J].食品工业,2014(12):226-229.
[20]Hausman G J,Dodson M V,Ajuwon K. Board-invited review:The biology and regulation of preadipocytes and adipocytes in meat animals[J]. Journal of Animal Science,2009,87(4):1218-1246.
[21]Alves SP,Bessa RJB,Quaesma MAG,et al. Does the fat tailed damara ovine breed have a distinct lipid metabolism leading to a high concentration of branched chain fatty acids in tissues?[J]Plos One,2013,8(10):1-9.
[22]Sebastien E,Alice D,Sandrine F,et al. Effect of a long-chain n-3 polyunsaturated fatty acid-enriched diet on adipose tissue lipid profiles and gene expression in Holstein dairy cows[J]. Journal of Dairy Science,2016,99(12):10109-10127.
[23]尹靖东. 动物肌肉生物学与肉品科学[M]. 北京:中国农业大学出版社,2011.
[24]Alexanader JK,Kaija JA;Juha MK,et al. Mitochondrial fatty acid synthesis,fatty acids and mitochondrial physiology[J]. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids,2017,1862(1):39-48.
[25]Castro CT,Frutos P,Leroux C,et al. Dietary sunflower oil modulates milk fatty acid composition without major changes in adipose and mammary tissue fatty acid Profile or related gene mRNA abundance in sheep[J]. Animal,2015,9(4):582-91.
[26]Hu G,Wang S,Tian J,et al. Epistatic effect between ACACA and FABP2 gene on abdominal fat traits in broilers[J]. Journal of Genetics and Genomics,2010,37(8):505.DOI:10.1016/S1673-8527(09)60070-9.
[27]张艳芳. CPT1基因对猪脂肪沉积的影响及其调控机制的研究[D].杭州:浙江大学,2010.
[28]梅秀丽.优质鸡不同饲养方式下FADS1、FADS2基因的表达及其对脂肪酸组成的影响[D].成都:四川农业大学,2012.
[29]曾瑛,唐蓓,李影. Micro RNAs在哺乳动物脂肪形成中的调控作用[J].中国细胞生物学学报,2015,37(1):1-9.
[30]Claudia G,Joachim H,Berthold K. Role of FADS1 and FADS2 polymorphisms in polyunsaturated fatty acid metabolism[J]. Metabolism,2016,59(7):993-996.
[31]Tananka T,Jian S,Abecasis GR,et al. Genome-wide association study of plasma polyunsaturated fatty acids in the InChianti Study[J]. Plos Genetics,2009,5(1):e1000338.
[32]Samulin J,Berget I,Grindflek E,et al. Changes in lipid metabolism associated gene transcripts during porcine adipogenesis[J]. Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology,2009,153(1):8-17.
[33]Madhu SR,Tyagi A,Hossai SA,et al. Effect of tanniniferous Terminalia chebula extract on rumen biohydrogenation,Δ9-desaturase activity,CLA content and fatty acid composition in longissimus dorsi muscle of kids[J].Meat Science,2012,90(3):558-563.