黄唇鱼消化系统组织结构及黏液细胞分布特征
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摘要
利用石蜡切片、HE和阿利新兰-高碘酸雪夫-试剂[AB-PAS (Alician blue and periodic acid Schiff reagent AB, pH 2.5)]染色技术,研究黄唇鱼(Bahaba flavolabiata)消化系统的组织结构和黏液细胞的分布特征。黄唇鱼食道粗而短,胃呈"卜"形,分为贲门部、盲囊部和幽门部,胃与肠的连接处有8条幽门盲囊,肠道在体腔内迂回2个曲折。光镜下观察到消化管管壁由外至内依次为外膜、肌肉层、黏膜下层和黏膜层。食道肌肉层很厚,黏膜层很薄,未见黏液细胞分布。胃部肌肉层和黏膜层均很厚,黏膜固有层分布胃腺,黏膜上皮为单层柱状上皮。幽门盲囊管腔很大,但肌肉层很薄。肠道肌肉层较厚,黏膜皱褶较细长,黏膜上皮细胞间可见圆形的黏液细胞。肝脏内弥散分布胰腺组织,肝小叶内可见大量糖原空泡。AB-PAS染色检测到胃部分布为红色的Ⅰ型黏液细胞;幽门盲囊黏膜上皮分布大量蓝色的黏液细胞,为Ⅱ型黏液细胞;前肠、中肠和后肠检测到大量Ⅱ型黏液细胞,并有少量Ⅰ型黏液细胞。黄唇鱼消化系统的组织结构以及黏液细胞分布特征体现了食性与结构功能相协调的特点。
This work aimed to investigate the histological structure and mucous cell distributing features in the digestive system of Bahaba flavolabiata. Conventional paraffin sections were used, with H&E and AB-PAS(Alcian blue and periodic acid Schiff reagent(AB, pH 2.5)) staining. The esophagus was crude and short, and the Bu-type stomach was divided into three portions: the cardiac stomach, fundic stomach, and pyloric stomach. There were eight pyloric caeca, and the intestine had two inflections in the body cavity. The histological analysis revealed that the digestive tract wall was composed of serosa,muscularis, submucosa, and mucosa, from the outer wall inwards. The muscularis of the esophagus was thick, but the mucosa was thin, and the epithelium did not have mucous. Muscularis and mucosa of the stomach were thick and composed of simple columnar epithelium, with a gastric gland found in the lamina propria. The pyloric caeca were characterized as having large cavity diameters, and numerous mucosa folds, with very thin muscularis layers. The muscularis in the intestine was thicker than that in the pyloric caeca, with numerous mucosa folds. Here, mucous cells were found in the epithelium. AB-PAS staining revealed that only type Ⅰ and type Ⅱ mucous cells were present in the entire digestive tract of B. flavolabiata, except in the esophagus. Numerous type Ⅰ mucous cells were found in the gastric gland and epithelium of the stomach. Only type Ⅱ mucous cells were found distributed in the epithelium of pyloric caeca mucosa. A large number of type Ⅱ and a smaller number of type Ⅰ mucous cells were found in the intestine. The former were distributed mostly on the surface of the epithelium and the latter in the basal layer of the epithelium. Characteristics of the histological structure and mucous cell distribution in digestive systems are in accordance with the habits and predacity of B. flavolabiata.
This work aimed to investigate the histological structure and mucous cell distributing features in the digestive system of Bahaba flavolabiata. Conventional paraffin sections were used, with H&E and AB-PAS(Alcian blue and periodic acid Schiff reagent(AB, pH 2.5)) staining. The esophagus was crude and short, and the Bu-type stomach was divided into three portions: the cardiac stomach, fundic stomach, and pyloric stomach. There were eight pyloric caeca, and the intestine had two inflections in the body cavity. The histological analysis revealed that the digestive tract wall was composed of serosa,muscularis, submucosa, and mucosa, from the outer wall inwards. The muscularis of the esophagus was thick, but the mucosa was thin, and the epithelium did not have mucous. Muscularis and mucosa of the stomach were thick and composed of simple columnar epithelium, with a gastric gland found in the lamina propria. The pyloric caeca were characterized as having large cavity diameters, and numerous mucosa folds, with very thin muscularis layers. The muscularis in the intestine was thicker than that in the pyloric caeca, with numerous mucosa folds. Here, mucous cells were found in the epithelium. AB-PAS staining revealed that only type Ⅰ and type Ⅱ mucous cells were present in the entire digestive tract of B. flavolabiata, except in the esophagus. Numerous type Ⅰ mucous cells were found in the gastric gland and epithelium of the stomach. Only type Ⅱ mucous cells were found distributed in the epithelium of pyloric caeca mucosa. A large number of type Ⅱ and a smaller number of type Ⅰ mucous cells were found in the intestine. The former were distributed mostly on the surface of the epithelium and the latter in the basal layer of the epithelium. Characteristics of the histological structure and mucous cell distribution in digestive systems are in accordance with the habits and predacity of B. flavolabiata.
引文
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Lin XZ,Wang PF,Ou YJ,et al.An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos.Aquaculture Research,2016,1-11
Mc Guckin MA,Lindén SK,Sutton P,et al.Mucin dynamics and enteric pathogens.Nature Reviews Microbiology,2011,9(4):265-278
Ou YJ,Gou XW,Li JE.Histological and histochemical studies on digestive system of Cromileptes altivelis.Marine Fisheries.2011;33(3):289-96[区又君,勾效伟,李加儿.驼背鲈消化系统组织学与组织化学研究.海洋渔业,2011a,33(3):289-296]
Ou YJ,Li JE,Gou XW.Morphological,histological,and histochemical observation of digestive tract in ovate pompano Trachinotus ovatus.Journal of Dalian Ocean University,2012,27(1):38-43[区又君,李加儿,勾效伟.卵形鲳鲹消化道的形态学,组织学和组织化学.大连海洋大学学报,2012a,27(1):38-43]
Ou YJ,Liao GY,Li JE.Morphology and histology of the digestive tract in Cheilinus undulatus.Journal of Tropical Oceanography,2012,31(6):83-89[区又君,廖光勇,李加儿.波纹唇鱼消化道的形态学和组织学.热带海洋学报,2012b,31(6):83-89]
Ou YJ,Liao R,Li JE,et al.Application of otolith morphology in species discrimination for Bahaba flavolabiata,Pseudosciaena crocea,wak tingi and Collichthys lucidus.Guangdong Agricultural Science,2012,39(24):143-147[区又君,廖锐,李加儿,等.耳石形态在黄唇鱼、大黄鱼、丁氏和棘头梅童鱼种类识别中的应用.广东农业科学,2012c,39(24):143-147]
Ou YJ,Liao R,Li JE,et al.Studies on the spawning period and growth of young Bahaba flavolabiata in estuary of Pearl River by otolith determination.Ecological Science,2014,33(2):209-212[区又君,廖锐,李加儿,等.采用耳石日轮研究珠江口黄唇鱼幼鱼的产卵期及生长.生态科学,2014,33(2):209-212]
Ou YJ,Liao R,Li JE,et al.The analysis and evaluation of nutrition composition in muscle and air bladder of wild Bahaba flavolabiata.Acta Oceanologica Sinica,2010,32(3):113-120[区又君,廖锐,李加儿,等.野生黄唇鱼Bahaba flavolabiata肌肉和鳔营养成分的分析与评价.海洋学报,2010,32(3):113-120]
Ou YJ,Liao R,Li JE,et al.The morphology of otolith and its characteristics of microstructure in Bahaba flavolabiata.Guangdong Agricultural Science,2011,38(12):123-124[区又君,廖锐,李加儿,等.黄唇鱼的耳石形态和微结构特征.广东农业科学,2011,38(12):123-124]
Wang YC,Li JE,Ou YJ,et al.Morphological and histological study on the digestive tract of wild and cultivated yellowfin black porgy(Sparus latus).Chinese Journal of Zoology,2012,47(3):9-19[王永翠,李加儿,区又君,等.野生与养殖黄鳍鲷消化道形态组织结构.动物学杂志,2012,47(3):9-19]
Wang YC,Li JE,Ou YJ,et al.Types and distribution of mucous cells in digestive tracts of wild and cultivated yellowfin black porgy(Sparus latus).South China Fisheries Science.2012,8(5):46-51[王永翠,李加儿,区又君,等.野生与养殖黄鳍鲷消化道中黏液细胞的类型及分布.南方水产科学,2012,8(5):46-51]
Xie MJ,Ou YJ,Li JE,et al.Histological observation of the post-embryonic development of digestive tract of eleutheronema tetradactylum.Progress In Fishery Sciences,2017,38(2):50-58[谢木娇,区又君,李加儿,等.四指马鲅(Eleutheronema tetradactylum)消化系统胚后发育组织学观察.渔业科学进展,2017,38(2):50-58]
Xie MJ,Ou YJ,Li JE,et al.A comparative histochemical study on mucous cells in digestive tract of Eleutheronema tetradactylum with juvenile,young and adult fish.Chinese Journal of Cell Biology,2015,37(9):1226-1234[谢木娇区又君,李加儿,等.四指马鲅稚鱼、幼鱼和成鱼消化道黏液细胞组织化学研究.中国细胞生物学学报,201537(9):1226-1234]
Xie MJ,Ou YJ,Li JE,et al.Histology observation in digestive tract of Eleutherollema tetradactylum at different developmental stages.South China Fisheries Science,201612(2):51-58[谢木娇,区又君,李加儿,等.不同发育阶段的四指马鲅消化道组织学比较研究.南方水产科学,2016,12(2):51-58]
Zeng R,Ye YT.Studies on digestive system and different feeding habits of some fishes in freshwater.Journal of Southwest Agricultural University,1998,20(4):361-364[曾端,叶元土.鱼类食性与消化系统结构的研究.西南农业大学学报,1998,20(4):361-364]
Zhong JS.Bahaba flavolabiata.The freshwater fishes of Guangdong Province,1991,380-381[钟俊生.黄唇鱼.广东淡水鱼类志,1991,380-381]
Chen BN,Qin JG,Kumar MS,et al.Ontogenetic development of the digestive system in yellowtail kingfish Seriola lalandi larvae.Aquaculture,2006,256(1-4):489-501
Chen MY,Zhang XM.Development of digestive system in larval,juvenile and young turbot Scophthalmus maximus.Acta Hydrobiologica Sinica,2006,30(2):236-240[陈慕雁,张秀梅.大菱鲆仔稚幼鱼消化系统发育的组织学研究.水生生物学报,2006,30(2):236-240]
Cui LB,Chi S,Li XH,et al.Histological and histological study of the digestive system of Pseudosciaene crocea.Journal of Yantai University(Natural Science and Engineering Edition).2014(4):266-270[崔龙波,迟爽,李新华,等.大黄鱼消化系统的组织学和组织化学研究.烟台大学学报(自然科学与工程版),2014(4):266-270]
Dong J,Liu C,Wu GF,et al.Morphological and histological studies in the digestive tract of Pelteobagrus fulvidraco.Heilongjiang Animal Science and Veterinary Medicine,2014(23):4-6[董婧,刘聪,吴高峰,等.黄颡鱼消化道形态与组织学研究.黑龙江畜牧兽医,2014(23):4-6]
Gou XW,Ou YJ,Liao R.Histological and histochemicalstudy of digestive system of Rhabdosargus sarba.South China Fisheries Science,2008,4(5):28-36[勾效伟,区又君,廖锐.平鲷消化系统形态学、组织学及组织化学研究.南方水产,2008,4(5):28-36]
Guan HH,Kuang YY,Xu W,et al.Morphological and histological observations of the digestive system in Hucho taimen.Journal of Fishery Sciences of China,2008,15(5):873-879[关海红,匡友谊,徐伟,等.哲罗鱼消化系统形态学和组织学观察.中国水产科学,2008,15(5):873-879]
Li XW,Luo J,Li WP,et al.Types and distribution of mucous cells in larval and juvenile digestive tract of Epinephelus malabaricus(Bloch&Schneider).Progress In Fishery Sciences,2012,33(5):15-23[李鑫炜,骆剑,李伟萍,等.点带石斑鱼仔稚鱼消化系统黏液细胞的类型与分布.渔业科学进展,2012,33(5):15-23]
Lin XZ,Wang PF,Ou YJ,et al.An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos.Aquaculture Research,2016,1-11
Mc Guckin MA,Lindén SK,Sutton P,et al.Mucin dynamics and enteric pathogens.Nature Reviews Microbiology,2011,9(4):265-278
Ou YJ,Gou XW,Li JE.Histological and histochemical studies on digestive system of Cromileptes altivelis.Marine Fisheries.2011;33(3):289-96[区又君,勾效伟,李加儿.驼背鲈消化系统组织学与组织化学研究.海洋渔业,2011a,33(3):289-296]
Ou YJ,Li JE,Gou XW.Morphological,histological,and histochemical observation of digestive tract in ovate pompano Trachinotus ovatus.Journal of Dalian Ocean University,2012,27(1):38-43[区又君,李加儿,勾效伟.卵形鲳鲹消化道的形态学,组织学和组织化学.大连海洋大学学报,2012a,27(1):38-43]
Ou YJ,Liao GY,Li JE.Morphology and histology of the digestive tract in Cheilinus undulatus.Journal of Tropical Oceanography,2012,31(6):83-89[区又君,廖光勇,李加儿.波纹唇鱼消化道的形态学和组织学.热带海洋学报,2012b,31(6):83-89]
Ou YJ,Liao R,Li JE,et al.Application of otolith morphology in species discrimination for Bahaba flavolabiata,Pseudosciaena crocea,wak tingi and Collichthys lucidus.Guangdong Agricultural Science,2012,39(24):143-147[区又君,廖锐,李加儿,等.耳石形态在黄唇鱼、大黄鱼、丁氏和棘头梅童鱼种类识别中的应用.广东农业科学,2012c,39(24):143-147]
Ou YJ,Liao R,Li JE,et al.Studies on the spawning period and growth of young Bahaba flavolabiata in estuary of Pearl River by otolith determination.Ecological Science,2014,33(2):209-212[区又君,廖锐,李加儿,等.采用耳石日轮研究珠江口黄唇鱼幼鱼的产卵期及生长.生态科学,2014,33(2):209-212]
Ou YJ,Liao R,Li JE,et al.The analysis and evaluation of nutrition composition in muscle and air bladder of wild Bahaba flavolabiata.Acta Oceanologica Sinica,2010,32(3):113-120[区又君,廖锐,李加儿,等.野生黄唇鱼Bahaba flavolabiata肌肉和鳔营养成分的分析与评价.海洋学报,2010,32(3):113-120]
Ou YJ,Liao R,Li JE,et al.The morphology of otolith and its characteristics of microstructure in Bahaba flavolabiata.Guangdong Agricultural Science,2011,38(12):123-124[区又君,廖锐,李加儿,等.黄唇鱼的耳石形态和微结构特征.广东农业科学,2011,38(12):123-124]
Wang YC,Li JE,Ou YJ,et al.Morphological and histological study on the digestive tract of wild and cultivated yellowfin black porgy(Sparus latus).Chinese Journal of Zoology,2012,47(3):9-19[王永翠,李加儿,区又君,等.野生与养殖黄鳍鲷消化道形态组织结构.动物学杂志,2012,47(3):9-19]
Wang YC,Li JE,Ou YJ,et al.Types and distribution of mucous cells in digestive tracts of wild and cultivated yellowfin black porgy(Sparus latus).South China Fisheries Science.2012,8(5):46-51[王永翠,李加儿,区又君,等.野生与养殖黄鳍鲷消化道中黏液细胞的类型及分布.南方水产科学,2012,8(5):46-51]
Xie MJ,Ou YJ,Li JE,et al.Histological observation of the post-embryonic development of digestive tract of eleutheronema tetradactylum.Progress In Fishery Sciences,2017,38(2):50-58[谢木娇,区又君,李加儿,等.四指马鲅(Eleutheronema tetradactylum)消化系统胚后发育组织学观察.渔业科学进展,2017,38(2):50-58]
Xie MJ,Ou YJ,Li JE,et al.A comparative histochemical study on mucous cells in digestive tract of Eleutheronema tetradactylum with juvenile,young and adult fish.Chinese Journal of Cell Biology,2015,37(9):1226-1234[谢木娇区又君,李加儿,等.四指马鲅稚鱼、幼鱼和成鱼消化道黏液细胞组织化学研究.中国细胞生物学学报,201537(9):1226-1234]
Xie MJ,Ou YJ,Li JE,et al.Histology observation in digestive tract of Eleutherollema tetradactylum at different developmental stages.South China Fisheries Science,201612(2):51-58[谢木娇,区又君,李加儿,等.不同发育阶段的四指马鲅消化道组织学比较研究.南方水产科学,2016,12(2):51-58]
Zeng R,Ye YT.Studies on digestive system and different feeding habits of some fishes in freshwater.Journal of Southwest Agricultural University,1998,20(4):361-364[曾端,叶元土.鱼类食性与消化系统结构的研究.西南农业大学学报,1998,20(4):361-364]
Zhong JS.Bahaba flavolabiata.The freshwater fishes of Guangdong Province,1991,380-381[钟俊生.黄唇鱼.广东淡水鱼类志,1991,380-381]