刺托竹荪菌盖碳量子点的制备及其抑菌性研究
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摘要
【目的】利用废旧生物质资源制备量子点并增加细菌病害防治的资源。【方法】以刺托竹荪菌盖采用水热法合成碳量子点,并用透射电镜扫描、红外光谱、XRD和荧光分析对碳量子点进行表征。选取青枯雷尔氏菌Ralstonia solanacearum和地毯草黄单胞菌Xanthomonas axonopodis作为供试菌种,采用抑菌圈法对碳量子点的抑菌性能进行研究。【结果】该方法制得的碳量子点近似球形,粒径均匀,分散性好。其发射波长依赖于激发波长,在紫外灯的照射下,出现明亮的蓝色荧光。碳量子点对青枯雷尔氏菌和地毯草黄单胞菌均有明显的抑菌作用。【结论】获得了一种可用于防治植物细菌病害的量子点。
【Objective】Utilization of mushroom biomass waste to prepare carbon dots(CDs)for the prevention and control of diseases on plants was studied.【Method】CDs were obtained by hydrothermal oxidation of pilei of Dictyophora echinovolvata and characterized by the transmission electron microscopy(TEM),Fourier transform infrared spectrophoto-metry(FT-IR),x-ray diffraction(XRD),and fluorescene spectrophotometry.Antibacterial properties of the CDs were determined by measurements of diameter of the bacteriostatic circle on Ralstonia solanacearum and Xanthomonas axonopodis. 【Result】The CDs obtained displayed an appropriate spherical morphology and uniform size with good dispersion.They emitted bright blue photoluminescence under UV excitation,exhibited an excitation-dependent PL behavior,and demonstrated excellent antibacterial activities against R.solanacearumand X.axonopodis.【Conclusion】The CDs with a potential of preventing and controlling plant diseases by bacterial infection were successful prepared in laboratory.
【Objective】Utilization of mushroom biomass waste to prepare carbon dots(CDs)for the prevention and control of diseases on plants was studied.【Method】CDs were obtained by hydrothermal oxidation of pilei of Dictyophora echinovolvata and characterized by the transmission electron microscopy(TEM),Fourier transform infrared spectrophoto-metry(FT-IR),x-ray diffraction(XRD),and fluorescene spectrophotometry.Antibacterial properties of the CDs were determined by measurements of diameter of the bacteriostatic circle on Ralstonia solanacearum and Xanthomonas axonopodis. 【Result】The CDs obtained displayed an appropriate spherical morphology and uniform size with good dispersion.They emitted bright blue photoluminescence under UV excitation,exhibited an excitation-dependent PL behavior,and demonstrated excellent antibacterial activities against R.solanacearumand X.axonopodis.【Conclusion】The CDs with a potential of preventing and controlling plant diseases by bacterial infection were successful prepared in laboratory.
引文
[1]李明亮.具有生物活性的量子点的制备及其应用[D].兰州:兰州理工大学,2015.LI M L.Preparation and application of quantum dots with biological activity[D]. Lanzhou:Lanzhou University of Technology,2015.(in Chinese)
[2]黄启同,林小凤,李飞明,等.碳量子点的合成与应用[J].化学进展,2015,27(11):1604-1614.HUANG Q T,LIN X F,LI F M,et al.Synthesis and applications of carbon dots[J].Progress in Chemistry,2015,27(11):1604-1614.(in Chinese)
[3]刘辰,冯文祥,胡倬铭,等.酶解木质素水热法制备碳量子点[J].广东化工,2017,44(12):15-16.LIU C,FENG W X,HU Z M,et al.Hydrothermal synthesis of fluorescent carbon dots from cellulolytic enzyme lignin[J].Guangdong Chemical Industry,2017,44(12):15-16.(in Chinese)
[4]陈文新,阳运华,刘应亮,等.基于生物质甘蔗渣的荧光碳量子点制备[J].暨南大学学报(自然科学与医学版),2015,36(1):1-6.CHEN W X,YANG Y H,LIU Y L,et al.Facile synthesis of fluorescent carbon nanodots derived from biomass bagasse by hydrothermal treatment[J].Journal of Jinan University,2015,36(1):1-6.(in Chinese)
[5]林陈强,林戎斌,陈济琛,等.棘托竹荪菌盖提取物的抗氧化活性[J].食用菌学报,2013,20(2):32-36.LIN C Q,LIN R B,CHEN J C,et al.Radical scavenging and Fe2+chelating activities of aqueous,ethanolic and ethyl acetate extracts of dictyophora echinovolvata pilei[J].Acta Edulis Fungi,2013,20(2):32-36.(in Chinese)
[6]DOU Q Q,FANG X,JIANG S,et al. Multi-functional fluorescent carbon dots with antibacterial and gene delivery properties[J].Rsc Advances,2015,5(58):46817-46822.
[7]李红霞,王兴域,关凤,等.碳量子点槲皮素复合物的制备及其抑菌性能研究[J].西北师范大学学报(自然科学版),2015(5):70-73.LI H X, WANG X Y, GUAN F, et al. Synthesis,characterization and antimicrobial activity of C-K H560-Quercetin composite[J].Journal of Northwest Normal University Natural Science,2015(5):70-73.(in Chinese)
[8]LIU J,LU S, TANG Q,et al. One-step hydrothermal synthesis of photoluminescent carbon nanodots with selective antibacterial activity against Porphyromonas gingivalis[J].Nanoscale,2017,9(21):7135.
[9]李林章,谢从华,柳俊.茄科雷尔氏菌(Ralstonia solanacearum)分子生物学基础及其致病机制[J].中国马铃薯,2005,19(5):290-294.LI L Z,XIE C H,LIU J.Molecular biology and pathogenicity of Ralstonia solanacearum[J].Chinese Potato,2005,19(5):290-294.(in Chinese)
[10]ZHENG X F,ZHU Y J,LIU B,et al.Relationship between Ralstonia solanacearum diversity and severity of bacterial wilt disease in tomato fields in China[J]. Journal of Phytopathology,2014,162(9):607-616.
[11]RAMESH R,PHADKE G S.Rhizosphere and endophytic bacteria for the suppression of eggplant wilt caused by Ralstonia solanacearum[J].Crop Protection,2012,37(2):35-41.
[12]程本亮.青枯雷尔氏菌(Ralstonia solanacearum)致病力分化的研究[D].福州:福建农林大学,2010.CHENG B L.The study of virulent strains of Ralstonia solanacearum[D].Fuzhou:Fujian Agriculture and Forestry University,2010.(in Chinese)
[13]谭军,刘雨虹,刘影,等.不同条件对烟草青枯雷尔氏菌生长的影响[J].中国烟草科学,2014,35(1):85-88.TAN J,LIU Y H,LIU Y,et al.Effects of different conditions on growth of tobacco Ralstonia solanacearum[J].Chinese Tobacco Science,2014,35(1):85-88.(in Chinese)
[14]钱韦.两种植物病原黄单胞菌基因组中同义密码子使用的分析[J].植物病理学报,2004,34(2):97-106.QIAN W. Analysis of synonymous codon usage in two phytopathogen genomes of Xanthomonas campestris pv.campestris and X. axonopodis pv. citri[J]. Acta Phytopathologica Sinica,2004,34(2):97-106.(in Chinese)
[15]KHODAKARAMIAN G,SWINGS J.AFLP fingerprinting of the strains of Xanthomonas axonopodis inducing citrus canker disease in southern iran[J].Journal of Phytopathology,2010,150(4-5):227-231.
[16]任希望,梁丽雯,王鹤鸣,等.木薯细菌性枯萎病病原菌的分离与鉴定[J].热带生物学报,2015,6(1):59-64.REN X W,LIANG L W,WANG H M,et al.Isolation and identification of cassava bacterial blight pathogen[J].Journal of South China University of Tropical Agriculture,2015,6(1):59-64.(in Chinese)
[17]OSKAGHI E,ZADEMOHAMAD A A.Phaseolus lunatus,a new host of Xanthomonas axonopodis pv.phaseoli in Iran[J].Journal of Phytopathology,2016,164(1):56-60.
[18]郑雪芳,刘波,朱育菁,等.番茄青枯病生防芽胞杆菌的筛选与鉴定[J].中国生物防治学报,2016,32(5):657-665.ZHENG X F,LIU B,ZHU Y J,et al.Selection and identification of biological control bacillus strains agaist plant bacterial wilt disease[J].Chinese Journal of Biological Control,2016,32(5):657-665.(in Chinese)
[19]林陈强,曹萌,陈济琛,等.棘托竹荪菌盖营养成分分析与评价[J].热带作物学报,2016(11):2089-2093.LIN C Q,CAO M,CHEN J C,et al.Analysis and evaluation of nutritional components of Dictyophora echinovolvata pileus[J].Chinese Journal of Tropical Crops,2016(11):2089-2093.(in Chinese)
[20]曹萌.棘托竹荪菌盖蛋白提取及分析[J].天然产物研究与开发,2014(4):475-479.CAO M.Study on extraction and analysis of protein from dictyophora echinovolvata pileus[J]. Natural Product Research and Development,2014(4):475-479.(in Chinese)
[21]庄永亮,孙丽平,尚小丽.红托竹荪菌盖多糖的提取及抗氧化能力的研究[J].林产化学与工业,2011,31(3):45-49.ZHUANG Y L,SUN L P,SHANG X L.Extraction and antioxidant activities of polysaccharides from pileus of Dictyophora rubrovalvata[J].Chemistry &Industry of Forest Products,2011,31(3):45-49.(in Chinese)
[22]檀东飞,黄儒珠,卢真,等.棘托竹荪菌盖的化学成分及抑菌作用研究(Ⅱ)[J].微生物学杂志,2007,27(6):8-12.TANG D F, HUANG R Z, LU Z,et al. Chemical compositions and antimicrobial activity of the pileus of Dictyophora echinovolvata(Ⅱ)[J]. Journal of Microbiology,2007,27(6):8-12.(in Chinese)
[23]姚廷山.中国柑桔溃疡病菌遗传多样性分析及其防治药剂筛选[D].重庆:西南大学,2009.YAO T S.Primary study on enomic diversity of Xanthomonas axonopodis pv.citri in nine provinces of China and screening of its control bactericides[D]. Chongqing:Southwest University,2009.(in Chinese)
[24]黄保宏,高正良,周本国,等.四种杀菌剂对烟草青枯病菌的毒力比较[J].中国烟草学报,2015(1):72-75.HUANG B H,GAO Z L,ZHOU B G,et al.Toxicity test of four fungicides against Ralstonia solanacearum[J].Acta Tabacaria Sinica,2015(1):72-75.(in Chinese)
[25]刘国红,刘波,林乃铨.青枯雷尔氏菌生防芽胞杆菌的快速筛选及其鉴定[J].中国生物防治学报,2013,29(3):473-480.LIU G H, LIU B, LIN N Q. Rapidly screening and identification of an antagonistic Bacillus sp.against Ralstonia solanacearum[J].Chinese Journal of Biological Control,2013,29(3):473-480.(in Chinese)
[26]于艳梅.几种植物源化合物对青枯菌的抑制作用及机理研究[D].重庆:西南大学,2015.YU Y M.The antibacterial effects and mechanism of several botanical compounds against Ralstonia solanacearum[D].Chongqing:Southwest University,2015.(in Chinese)
[2]黄启同,林小凤,李飞明,等.碳量子点的合成与应用[J].化学进展,2015,27(11):1604-1614.HUANG Q T,LIN X F,LI F M,et al.Synthesis and applications of carbon dots[J].Progress in Chemistry,2015,27(11):1604-1614.(in Chinese)
[3]刘辰,冯文祥,胡倬铭,等.酶解木质素水热法制备碳量子点[J].广东化工,2017,44(12):15-16.LIU C,FENG W X,HU Z M,et al.Hydrothermal synthesis of fluorescent carbon dots from cellulolytic enzyme lignin[J].Guangdong Chemical Industry,2017,44(12):15-16.(in Chinese)
[4]陈文新,阳运华,刘应亮,等.基于生物质甘蔗渣的荧光碳量子点制备[J].暨南大学学报(自然科学与医学版),2015,36(1):1-6.CHEN W X,YANG Y H,LIU Y L,et al.Facile synthesis of fluorescent carbon nanodots derived from biomass bagasse by hydrothermal treatment[J].Journal of Jinan University,2015,36(1):1-6.(in Chinese)
[5]林陈强,林戎斌,陈济琛,等.棘托竹荪菌盖提取物的抗氧化活性[J].食用菌学报,2013,20(2):32-36.LIN C Q,LIN R B,CHEN J C,et al.Radical scavenging and Fe2+chelating activities of aqueous,ethanolic and ethyl acetate extracts of dictyophora echinovolvata pilei[J].Acta Edulis Fungi,2013,20(2):32-36.(in Chinese)
[6]DOU Q Q,FANG X,JIANG S,et al. Multi-functional fluorescent carbon dots with antibacterial and gene delivery properties[J].Rsc Advances,2015,5(58):46817-46822.
[7]李红霞,王兴域,关凤,等.碳量子点槲皮素复合物的制备及其抑菌性能研究[J].西北师范大学学报(自然科学版),2015(5):70-73.LI H X, WANG X Y, GUAN F, et al. Synthesis,characterization and antimicrobial activity of C-K H560-Quercetin composite[J].Journal of Northwest Normal University Natural Science,2015(5):70-73.(in Chinese)
[8]LIU J,LU S, TANG Q,et al. One-step hydrothermal synthesis of photoluminescent carbon nanodots with selective antibacterial activity against Porphyromonas gingivalis[J].Nanoscale,2017,9(21):7135.
[9]李林章,谢从华,柳俊.茄科雷尔氏菌(Ralstonia solanacearum)分子生物学基础及其致病机制[J].中国马铃薯,2005,19(5):290-294.LI L Z,XIE C H,LIU J.Molecular biology and pathogenicity of Ralstonia solanacearum[J].Chinese Potato,2005,19(5):290-294.(in Chinese)
[10]ZHENG X F,ZHU Y J,LIU B,et al.Relationship between Ralstonia solanacearum diversity and severity of bacterial wilt disease in tomato fields in China[J]. Journal of Phytopathology,2014,162(9):607-616.
[11]RAMESH R,PHADKE G S.Rhizosphere and endophytic bacteria for the suppression of eggplant wilt caused by Ralstonia solanacearum[J].Crop Protection,2012,37(2):35-41.
[12]程本亮.青枯雷尔氏菌(Ralstonia solanacearum)致病力分化的研究[D].福州:福建农林大学,2010.CHENG B L.The study of virulent strains of Ralstonia solanacearum[D].Fuzhou:Fujian Agriculture and Forestry University,2010.(in Chinese)
[13]谭军,刘雨虹,刘影,等.不同条件对烟草青枯雷尔氏菌生长的影响[J].中国烟草科学,2014,35(1):85-88.TAN J,LIU Y H,LIU Y,et al.Effects of different conditions on growth of tobacco Ralstonia solanacearum[J].Chinese Tobacco Science,2014,35(1):85-88.(in Chinese)
[14]钱韦.两种植物病原黄单胞菌基因组中同义密码子使用的分析[J].植物病理学报,2004,34(2):97-106.QIAN W. Analysis of synonymous codon usage in two phytopathogen genomes of Xanthomonas campestris pv.campestris and X. axonopodis pv. citri[J]. Acta Phytopathologica Sinica,2004,34(2):97-106.(in Chinese)
[15]KHODAKARAMIAN G,SWINGS J.AFLP fingerprinting of the strains of Xanthomonas axonopodis inducing citrus canker disease in southern iran[J].Journal of Phytopathology,2010,150(4-5):227-231.
[16]任希望,梁丽雯,王鹤鸣,等.木薯细菌性枯萎病病原菌的分离与鉴定[J].热带生物学报,2015,6(1):59-64.REN X W,LIANG L W,WANG H M,et al.Isolation and identification of cassava bacterial blight pathogen[J].Journal of South China University of Tropical Agriculture,2015,6(1):59-64.(in Chinese)
[17]OSKAGHI E,ZADEMOHAMAD A A.Phaseolus lunatus,a new host of Xanthomonas axonopodis pv.phaseoli in Iran[J].Journal of Phytopathology,2016,164(1):56-60.
[18]郑雪芳,刘波,朱育菁,等.番茄青枯病生防芽胞杆菌的筛选与鉴定[J].中国生物防治学报,2016,32(5):657-665.ZHENG X F,LIU B,ZHU Y J,et al.Selection and identification of biological control bacillus strains agaist plant bacterial wilt disease[J].Chinese Journal of Biological Control,2016,32(5):657-665.(in Chinese)
[19]林陈强,曹萌,陈济琛,等.棘托竹荪菌盖营养成分分析与评价[J].热带作物学报,2016(11):2089-2093.LIN C Q,CAO M,CHEN J C,et al.Analysis and evaluation of nutritional components of Dictyophora echinovolvata pileus[J].Chinese Journal of Tropical Crops,2016(11):2089-2093.(in Chinese)
[20]曹萌.棘托竹荪菌盖蛋白提取及分析[J].天然产物研究与开发,2014(4):475-479.CAO M.Study on extraction and analysis of protein from dictyophora echinovolvata pileus[J]. Natural Product Research and Development,2014(4):475-479.(in Chinese)
[21]庄永亮,孙丽平,尚小丽.红托竹荪菌盖多糖的提取及抗氧化能力的研究[J].林产化学与工业,2011,31(3):45-49.ZHUANG Y L,SUN L P,SHANG X L.Extraction and antioxidant activities of polysaccharides from pileus of Dictyophora rubrovalvata[J].Chemistry &Industry of Forest Products,2011,31(3):45-49.(in Chinese)
[22]檀东飞,黄儒珠,卢真,等.棘托竹荪菌盖的化学成分及抑菌作用研究(Ⅱ)[J].微生物学杂志,2007,27(6):8-12.TANG D F, HUANG R Z, LU Z,et al. Chemical compositions and antimicrobial activity of the pileus of Dictyophora echinovolvata(Ⅱ)[J]. Journal of Microbiology,2007,27(6):8-12.(in Chinese)
[23]姚廷山.中国柑桔溃疡病菌遗传多样性分析及其防治药剂筛选[D].重庆:西南大学,2009.YAO T S.Primary study on enomic diversity of Xanthomonas axonopodis pv.citri in nine provinces of China and screening of its control bactericides[D]. Chongqing:Southwest University,2009.(in Chinese)
[24]黄保宏,高正良,周本国,等.四种杀菌剂对烟草青枯病菌的毒力比较[J].中国烟草学报,2015(1):72-75.HUANG B H,GAO Z L,ZHOU B G,et al.Toxicity test of four fungicides against Ralstonia solanacearum[J].Acta Tabacaria Sinica,2015(1):72-75.(in Chinese)
[25]刘国红,刘波,林乃铨.青枯雷尔氏菌生防芽胞杆菌的快速筛选及其鉴定[J].中国生物防治学报,2013,29(3):473-480.LIU G H, LIU B, LIN N Q. Rapidly screening and identification of an antagonistic Bacillus sp.against Ralstonia solanacearum[J].Chinese Journal of Biological Control,2013,29(3):473-480.(in Chinese)
[26]于艳梅.几种植物源化合物对青枯菌的抑制作用及机理研究[D].重庆:西南大学,2015.YU Y M.The antibacterial effects and mechanism of several botanical compounds against Ralstonia solanacearum[D].Chongqing:Southwest University,2015.(in Chinese)