高效石油降解菌的筛选及在石油污水处理中的应用
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摘要
分别从新疆油田、大庆油田的原油中分离得到高效石油降解菌2株,分别编号L-1和D-2,对菌株进行形态学、生理生化及分子生物学分析,而后将L-1和D-2等比例形成微生物复合菌剂,测定复合菌剂对不同浓度和不同性质原油的降解率,并将该复合菌剂初步应用于石油污水的处理中。经鉴定,这两株菌分别为多食鞘氨醇杆菌(Sphingobacterium multivorum)和动胶菌(Zoogloea oryzae)。复合菌剂的最适接种量为9%、最适处理时间为72 h、最适p H 7.5,在该条件下,可将污水的含油量降低85%。因此,这两株高效石油降解菌在石油污水的处理中具有巨大的应用潜力。
Two strains(L-1,D-2),which can degrade petroleum, were isolated from crude oil of Xinjiang oilfield and Daqing oilfield respectively. After the morphological, physiological, biochemical and molecular analysis, the two strains were identified and mixed together in equal proportion to form the microbial agents. Then, the microbial degradation rates of crude oil with different concentration and properties were tested. After that, the microbial agents were preliminarily applied to crude oil sewage disposal. As was shown in the results, the strains(L-1,D-2)were identified as Sphingobacterium multivorum and zoogloea oryzae respectively. The optimal inoculating concentration of the microbial agents was 9%, the optimal processing time was 6 h, and optimal p H was 7.5.Under that condition, the removal rate of crude oil could reach 85%. Therefore, the two oil-degrading bacterium were considered to have great utilization potentials on oil sewage disposal.
Two strains(L-1,D-2),which can degrade petroleum, were isolated from crude oil of Xinjiang oilfield and Daqing oilfield respectively. After the morphological, physiological, biochemical and molecular analysis, the two strains were identified and mixed together in equal proportion to form the microbial agents. Then, the microbial degradation rates of crude oil with different concentration and properties were tested. After that, the microbial agents were preliminarily applied to crude oil sewage disposal. As was shown in the results, the strains(L-1,D-2)were identified as Sphingobacterium multivorum and zoogloea oryzae respectively. The optimal inoculating concentration of the microbial agents was 9%, the optimal processing time was 6 h, and optimal p H was 7.5.Under that condition, the removal rate of crude oil could reach 85%. Therefore, the two oil-degrading bacterium were considered to have great utilization potentials on oil sewage disposal.
引文
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[3]吴莉娜,陈家庆,程继坤,等.石油化工污水处理技术研究[J].科学技术与工程,2013,13(15):4313-4316.
[4]张忠智,管仁军,卢晓艳,等.高矿化度油田污水的微生物处理中间试验[J].石油化工高等学校学报,2006,19(4):20-26.
[5]李清心,康从宝,王浩,等.利用石油微生物处理石油污水的初步实验[J].工业水处理,2003,23(12):13-15.
[6]吕勃熠,刘金彪,孙田甜,等.2株石油污染降解菌的分离与鉴定[J].天津理工大学学报,2014,30(1):60-64.
[7]王成娟.石油烃类组成的GC-MS分析[J].分析仪器,2012(4):92-99.
[8]布坎南R E,吉本斯N E.伯杰细菌鉴定手册[M].第八版.中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组,译.北京:科学出版社,1984.
[9]FANI R,BANDI C,BAZZICALUPO M,et al.Phylogeny of the genus Azospirilllum based on 16S r DNA sequence[J].FEMS Microbiol Letters,1995,129:195-200.
[10]HAN J,SUN L,DONG X,et al.Characterization of a novel plant growth-promoting bacteria strain Delftia tsuruhatensis HR4 both as a diazotroph and a potential biocontrol agent against various plant pathogens[J].Syst Appl Microbiol,2005,28(1):66-76.
[11]杨雪莲,李凤梅,刘婉婷,等.高效石油降解菌的筛选及其降解特性[J].农业环境科学学报,2008,27(1):230-233.
[12]余薇.石油降解微生物的筛选及其降解特性的研究[D].武汉:华中农业大学,2013.
[13]齐永强,王红旗,刘敬奇,等.土壤石油微生物降解影响因子的正交实验分析[J].地球学报,2003,24(3):279-284.
[14]姜筭,高伟,李倩,等.南海高效石油降解菌的筛选及降解特性研究[J].环境科学学报,2012,32(7):1572-1578.
[15]徐冯楠.高效石油降解菌筛选、降解特性分析及应用研究[D].陕西杨凌:西北农林科技大学,2010.
[16]刘继朝,崔岩山,张燕平,等.植物与微生物对石油污染土壤修复的影响[J].生态与农村环境学报,2009,25(2):80-83.