不同激素对油用牡丹上胚轴萌发的影响
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摘要
以‘凤丹白’胚根大于3cm的种子为试验材料,采用全因子试验方法,研究了GA3、NAA和6-BA 3种激素的不同浓度和时间对胚根种子的萌芽率、发芽指数和初萌期的影响,以期为苗木生产提供依据。结果表明:GA3500mg·L~(-1)处理16h,其萌芽率、发芽指数和初萌期分别为98.89%、0.28和77.00d,发芽指数显著高于对照、初萌期显著比对照提早了13.33d;NAA 100mg·L~(-1)和6-BA 100mg·L~(-1)对‘凤丹白’上胚轴萌发均没有明显的促进或抑制作用,300mg·L~(-1) NAA和6-BA、500mg·L~(-1) NAA和6-BA不论浸种时间长短均明显地抑制了‘凤丹白’种子的萌芽率和发芽指数;同时,500mg·L~(-1)NAA显著地延长了‘凤丹白’种子的初萌期,说明500mg·L~(-1) NAA严重抑制了‘凤丹白’种子的生长发育。
To improve the germination rate and germination regularity of Paeonia ostii ‘Feng Dan Bai'and provide theoretical basis for seedling production,the effects of different densities and treated time of 3hormones(GA3,NAA and 6-BA respectively)on germination rate,germination index and first sprouting day of seeds of Paeonia ostii ‘Feng Dan Bai'with radicle length greater than 3cm were studied using full factor test method.The results showed that germination rate,germination index and first sprouting day of the seeds treated for 16 hours in 500 mg·L~(-1) GA3 were 98.89%,0.28and77.00 days respectively.The germination index was significantly higher than that of control experiment,and the first sprouting day arrived 13.33 days earlier than the control.However,there were no significant effects of NAA and 6-BA at low concentration 100 mg·L~(-1) on the hypocotyl growth of Paeonia ostii ‘Feng Dan Bai'.The two treatments of 300 mg·L~(-1) NAA and 6-BA treatment,500 mg·L~(-1) NAA and 6-BA treatment both had significantly inhibitory effects on the germination rate and germination index of Paeonia ostii ‘Feng Dan Bai',regardless of the duration of seed soaking.Meanwhile,the 500mg·L~(-1) NAA treatment significantly extended the first sprouting day,suggesting that the growth of Paeonia ostii seeds were seriously restrained by 500mg·L~(-1) NAA treatment.
To improve the germination rate and germination regularity of Paeonia ostii ‘Feng Dan Bai'and provide theoretical basis for seedling production,the effects of different densities and treated time of 3hormones(GA3,NAA and 6-BA respectively)on germination rate,germination index and first sprouting day of seeds of Paeonia ostii ‘Feng Dan Bai'with radicle length greater than 3cm were studied using full factor test method.The results showed that germination rate,germination index and first sprouting day of the seeds treated for 16 hours in 500 mg·L~(-1) GA3 were 98.89%,0.28and77.00 days respectively.The germination index was significantly higher than that of control experiment,and the first sprouting day arrived 13.33 days earlier than the control.However,there were no significant effects of NAA and 6-BA at low concentration 100 mg·L~(-1) on the hypocotyl growth of Paeonia ostii ‘Feng Dan Bai'.The two treatments of 300 mg·L~(-1) NAA and 6-BA treatment,500 mg·L~(-1) NAA and 6-BA treatment both had significantly inhibitory effects on the germination rate and germination index of Paeonia ostii ‘Feng Dan Bai',regardless of the duration of seed soaking.Meanwhile,the 500mg·L~(-1) NAA treatment significantly extended the first sprouting day,suggesting that the growth of Paeonia ostii seeds were seriously restrained by 500mg·L~(-1) NAA treatment.
引文
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[15]娄方芳,赵林森,李宗艳.昆明西山野生黄牡丹种子休眠与萌发特性初步研究[J].西南林学院学报,2007,27(5):34-37.
[16]范丙友,高水平,史国安,等.赤霉素和低温打破牡丹上胚轴休眠技术研究[J].种子,2007,26(3):1-3.
[17]谭逸明,黄雨清,王千,等.不同处理对凤丹种子萌发的影响[J].中国种业,2009(1):38-40.
[18]钱仙云,徐迎春,柴翠翠,等.低温和GA3处理对凤丹种子萌发及幼苗质量影响[J].林业科技开发,2009,23(6):48-51.
[2]陈慧玲,杨彦伶,张新叶,等.油用牡丹研究进展[J].湖北林业科技,2013,42(5):41-44.
[3]任鸿雁.油用牡丹的种子萌发和容器育苗试验研究[D].北京:北京林业大学,2016.
[4]周琳,王雁.我国油用牡丹开发利用现状及产业化发展对策[J].世界林业研究,2014(1):68-71.
[5]增秀丽.西藏油用牡丹的生产和研究进展[J].西藏农业科技,2016,38(1):9-11.
[6]李育材.我的“中国梦”[N].中国绿色时报,2013-11-5(A02).
[7]郑相穆,周阮宝,谷丽萍,等.凤丹种子的休眠和萌发特性[J].植物生理学通讯,1995,31(4):260-262.
[8]周仁超,姚崇怀,潘俊,等.紫斑牡丹休眠和萌发特性初步研究[J].湖北农业科学,2002(1):59-60.
[9]郭丽萍.种子休眠原因及休眠解除方法研究[D].杨凌:西北农林科技大学,2016.
[10]王莲英.中国牡丹品种图志[M].北京:中国林业出版社,1997.
[11]林松明,徐迎春,蔡志仁,等.打破凤丹种子上胚轴休眠的研究[J].江苏农业科学,2006(1):84-86.
[12]张玉刚,郭绍霞,王莲英.牡丹容器育苗的初步研究[J].中国农学通报,2004,20(1):182-184.
[13]陶嘉陵,郑光华.种子活力[M].北京:科学出版社,1991.
[14]成仿云,李嘉钰.中国牡丹的输出及其在国外的发展Ⅰ:栽培牡丹[J].西北师范大学学报(自然科学版),1998(1):112-119.
[15]娄方芳,赵林森,李宗艳.昆明西山野生黄牡丹种子休眠与萌发特性初步研究[J].西南林学院学报,2007,27(5):34-37.
[16]范丙友,高水平,史国安,等.赤霉素和低温打破牡丹上胚轴休眠技术研究[J].种子,2007,26(3):1-3.
[17]谭逸明,黄雨清,王千,等.不同处理对凤丹种子萌发的影响[J].中国种业,2009(1):38-40.
[18]钱仙云,徐迎春,柴翠翠,等.低温和GA3处理对凤丹种子萌发及幼苗质量影响[J].林业科技开发,2009,23(6):48-51.