利用16个微卫星标记分析6个鸭品种的遗传多样性
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摘要
【目的】为系统评价润州凤头白鸭、连城白鸭、北京鸭、樱桃谷鸭、吉安红毛鸭和于田麻鸭6个鸭品种的遗传多样性。【方法】利用16个微卫星位点(STR分型技术)对6个鸭品种(各30只)的遗传多样性参数进行检测并进行聚类分析。【结果】16个微卫星位点共检测到141个等位基因,多态信息含量(PIC)介于0.25～0.60之间,其中,9个座位为高度多态位点(PIC>0.50),7个座位为中度多态位点(0.25 【Objective】To systematically evaluate the genetic diversity of the six duck breeds,namely Runzhou Crested White Duck,Liancheng White Duck,Peking Duck,Cherry Valley Duck,Ji'an Red Haired Duck and Yutian Ma Duck.【Method】Sixteen microsatellite loci(STR typing technique) were used to detect the genetic diversity parameters of six duck breeds(30 ducks each breed).【Result】A total of 141 alleles were detected at 16 microsatellite loci,and the polymorphic information content(PIC)ranged from 0.25 to 0.60. Among them,9 loci were highly polymorphic loci(PIC>0.50),7 loci were moderately polymorphic loci(0.25
引文
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[7]李应丰.连城白鸭遗传资源调查报告[J].中国畜禽种业,2018(3):134-136.
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[10]朱志明,陈晖,郑嫩珠,等.利用8个微卫星标记分析7个鸭群体的遗传多样性[J].中国畜牧杂志,2010,46(17):10-13.
[11]李慧芳,李碧春,陈宽维,等.中国地方鸭品种资源的分子遗传多样性[J].畜牧兽医学报,2006,37(11):1107-1113.
[12]张扬.我国部分地方鸭品种遗传多样性与群体结构分析[D].扬州:扬州大学,2014.
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[17]肖天放,柯柳玉,张力,等.利用微卫星标记研究鸭品种的遗传多样性[J].应用生态学报,2009,20(1):190-196.
[2]ROMANOV M N,WEIGEND S.Genetic diversity in chicken populations based on microsatellite markers[A].In Proceedings of the conference"From Jay Lush to Genomics:Vidions for Animal Breeding and Genetics"[C].USA:Ames Iowa,1999,17.
[3]吴艳,侯水生,刘小林,等.4个微卫星标记分析6个鸭群体之间的遗传关系[J].畜牧兽医学报,2006,37(4):389-395.
[4]张燕,方琪,陈兴勇,等.利用微卫星标记分析4个番鸭品系的遗传多样性[J].浙江农业科学,2015,56(4):558-560.
[5]张扬,朱振,黄种彬,等.福建省5个地方鸭品种的遗传多样性分析[J].中国畜牧杂志,2013,49(23):18-22.
[6]王彬.凤头白鸭与连城白鸭生产性能、遗传结构和部分凤头性状相关基因比较[D].扬州:扬州大学,2015.
[7]李应丰.连城白鸭遗传资源调查报告[J].中国畜禽种业,2018(3):134-136.
[8]尹郁荪.肉蛋兼用型特色鸭种:吉安红毛鸭[J].农村百事通,2004(16):36.
[9]J.萨姆布鲁克,DW拉塞尔,Joseph Sam brook,等.分子克隆实验指南[M].3版.北京:科学出版社,2002:463-470.
[10]朱志明,陈晖,郑嫩珠,等.利用8个微卫星标记分析7个鸭群体的遗传多样性[J].中国畜牧杂志,2010,46(17):10-13.
[11]李慧芳,李碧春,陈宽维,等.中国地方鸭品种资源的分子遗传多样性[J].畜牧兽医学报,2006,37(11):1107-1113.
[12]张扬.我国部分地方鸭品种遗传多样性与群体结构分析[D].扬州:扬州大学,2014.
[13]BOTESTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphismas[J].Am J Hum Genet,1980,32:313-331.
[14]NEI M,ROY CHOUDHURY A K.Sampling variances of heterozygosity and genetic distance[J].Genetics,1974,76:379-390.
[15]NEI M.Genetic distance between populations[J].American Naturalist,1972,106(949):283-292.
[16]鄢绯寰,左正宏,吕良炬,等.我国几种野鸭和家鸭遗传多样性的SSR分析[J].厦门大学学报(自然科学版),2009,48(3):435-440.
[17]肖天放,柯柳玉,张力,等.利用微卫星标记研究鸭品种的遗传多样性[J].应用生态学报,2009,20(1):190-196.