响应曲面法优化昆仑雪菊皂苷提取
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摘要
以昆仑雪菊为原料,对超声辅助提取昆仑雪菊皂苷的工艺进行优化研究。利用Box-Behnken的中心组合设计原理及响应面法(RSM)分析建立二次回归模型,对水料比、提取时间和提取温度3因素进行优化组合。确定超声辅助提取昆仑雪菊皂苷的最佳工艺条件为水料比21∶1(mL∶g)、超声温度61℃、超声时间29min,在该最佳工艺条件下,雪菊皂苷的提取率为7.57%。
Saponins in Coreopsis tinctoria were extracted with distilled water under the assistance of ultrasonic,and the extraction process was optimized by using response surface analysis based on Box-Behnken design to examine the effects of extraction conditions including liquid-to-material ratio,length of ultrasonic treatment time and extraction temperature on saponins yield,which were reflected by a quadratic regression model.The results showed that the optimal extraction conditions were: material-to-liquid ratio 1∶21(g∶mL),ultrasonic treatment time 29min and extraction temperature 61℃.Under these conditions,the saponins yield was 7.57%.
Saponins in Coreopsis tinctoria were extracted with distilled water under the assistance of ultrasonic,and the extraction process was optimized by using response surface analysis based on Box-Behnken design to examine the effects of extraction conditions including liquid-to-material ratio,length of ultrasonic treatment time and extraction temperature on saponins yield,which were reflected by a quadratic regression model.The results showed that the optimal extraction conditions were: material-to-liquid ratio 1∶21(g∶mL),ultrasonic treatment time 29min and extraction temperature 61℃.Under these conditions,the saponins yield was 7.57%.
引文
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[3]徐丽萍.桔梗总皂苷降血脂作用的研究[J].食品工业科技,2007(8):224-236.
[4]张崇泉,杨晓慧,徐琳本,等.绞股蓝总皂苷免疫调节作用的研究[J].中西医结合杂志,1990(2):96-97.
[5]CHANG W T,SHAO Z H,YIN J J,et al.Comparative effects of flavonoidson oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes[J].Eur J Pharmacol,2007,566(1/3):58-66.
[6]戚向阳,陈维军,张俐勤,等.罗汉果皂苷清除自由基及抗脂质过氧化作用的研究[J].2006,39(2):382-388.
[7]刘红梅,李瑞雪,杨学军,等.人参皂苷的神经保护作用和机制及其在神经变性疾病中的应用[J].大连医科大学学报,2009(5):595-598.
[8]张彦丽,韩艳春,阿依吐伦·斯马.分光光度法测定维吾尔药昆仑雪菊中总皂苷的含量[J].西北药学杂志,2011,26(2):87-88.
[9]覃艳,乐龙,王志祥.响应曲面法优化微波提取辣椒红色素的工艺[J].中国酿造,2011,30(2):100-103.
[10]王占良,王弘,陈世忠.高效液相色谱-二极管阵列检测/质谱法分析生脉饮煎剂中的人参皂苷类成分[J].色谱,2006,24(4):325-330.
[11]周纪芗.实用回归分析方法[M].上海:上海科学技术出版社,1990.
[12]潘青友,童群义.响应曲面法优化超声-微波协同提取百合渣中总皂苷工艺的研究[J].食品工业技术,2012,33(16):273-276.
[13]ANNADURAI G,SHEEJA RY.Use of Box-Behken design of experimentsfor the adsorption of verofix red using biopolymer[J].Bioprocess Eng,1998,18(6):463-466.
[14]AGHAIE E,PAZOUKI M,HOSSEINI MR,et al.Response surfacemethodology(RSM)analysis of organic acid production for Kalinbeneficiation by Aspergillus niger[J].Chem Eng J,2009,147(2-3):245-251.
[15]吴祥庭,朱涛,郑巧敏,等.响应面法优化山药皮中皂苷提取的研究[J].中国粮油学报,2011,26(6):90-94.
[16]MONTGOMERY D C.实验设计与分析[M].北京:中国统计出版社,1998.