响应面法优化荖叶精油的提取工艺及精油化学成分的分析
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摘要
利用同时蒸馏萃取技术,通过单因素实验优化,研究了物料粒径、料液比、超声时间、蒸馏时间、加盐量5个萃取条件对荖叶精油提取率的影响。在此基础上,使用响应面软件Design-Expert,选用BoxBehnken法(BBD)的四水平三因素对荖叶精油试验因素进行最佳优化,并采用气相色谱-质谱法(GCMS)对提取出的精油进行了成分分析。实验结果表明:荖叶精油的最佳提取条件为:破碎粒径64.50目,液料比8.29∶1,原料超声时间51.05 min,同时蒸馏萃取时间8.54h,响应面理论最优值21.87mg/g,实验验证平均值21.65mg/g。GC-MS对精油化学成分进行分析,共检测出41种物质,其中异丁香酚含量70.03%,对烯丙基苯酚含量10.52%,乙酰基丁香酚含量7.04%。
Simultaneous distillation extraction(SED)method is used to extract essential oil from leaves of Piper betle Linn.General levels of five extraction factors including particle size of material,solid to liquid ratio,ultrasonic time,distillation time and additive amount of salt are tested according to single factor experiment.Based on the previous results,four factors and three levels model is designed by response surface design expert software using Box-Benhnken Design(BBD)method to extract essential oil.At last,the components of extracted essential oil are analyzed by agas chromatograph mass spectrometer(GC-MS). The results indicate that the optimized theoretical conditions for extracting essential oil from leaves of Piper betle Linn.by SDE method are as follows:particle size of material is 64.50 meshes,liquid to solid ratio is 8.29∶1,ultrasonic treatment time is 51.05 min,SED time is 8.54 h,the additive amount of salt is 80g/L.The theoretical and real response values are21.87mg/g and 21.65 mg/g respectively.In the GC-MS analysis,41 compounds are detected,in which,isoeugenol,allyl phenol and acetyl eugenol is up to 70.03%,10.52%and 7.04%respectively.
Simultaneous distillation extraction(SED)method is used to extract essential oil from leaves of Piper betle Linn.General levels of five extraction factors including particle size of material,solid to liquid ratio,ultrasonic time,distillation time and additive amount of salt are tested according to single factor experiment.Based on the previous results,four factors and three levels model is designed by response surface design expert software using Box-Benhnken Design(BBD)method to extract essential oil.At last,the components of extracted essential oil are analyzed by agas chromatograph mass spectrometer(GC-MS). The results indicate that the optimized theoretical conditions for extracting essential oil from leaves of Piper betle Linn.by SDE method are as follows:particle size of material is 64.50 meshes,liquid to solid ratio is 8.29∶1,ultrasonic treatment time is 51.05 min,SED time is 8.54 h,the additive amount of salt is 80g/L.The theoretical and real response values are21.87mg/g and 21.65 mg/g respectively.In the GC-MS analysis,41 compounds are detected,in which,isoeugenol,allyl phenol and acetyl eugenol is up to 70.03%,10.52%and 7.04%respectively.
引文
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[4]王菁,张园,袁媛,等.5种海南岛药用植物抗肿瘤活性部位的筛选[J].海南师范大学学报:自然科学版,2011,24(3):301-306.
[5]廖超林.泰国蒌叶精油化学成分的研究[J].香料香精化妆品,2000(2):3-6.
[6]Chaintreau A.Simultaneous distillation-extraction:from birth to maturity—review[J].Flavor and Fragrance Journal,2001,16(2):136-148.
[7]Cai J,Liu B,Su Q.Comparison of simultaneous distillation extraction and solid-phase microextraction for the determination of volatile flavor components[J].Journal of Chromatography A,2001,930(1):1-7.
[8]Teixeira S,Mendes A,Alves A,et al.Simultaneous distillation-extraction of high-value volatile compounds from Cistus ladanifer L[J].Analytica Chimica Acta,2007,584(2):439-446.
[9]De Frutos M,Sanz J,Martínez-Castro I.Simultaneous distillation-extraction(SDE)method in the qualitative and quantitative GC analysis of cheese volatile components[J].Chromatographia,1988,25(10):861-864.
[10]Khuri A I,Mukhopadhyay S.Response surface methodology[J].Wiley Interdisciplinary Reviews:Computational Statistics,2010(2):128-149.
[11]Thompson D.Response surface experimentation[J].Journal of Food Processing and Preservation,1982,6(3):155-188.
[12]Myers W R,Montgomery D C.Response surface methodology[J].Encycl Biopharm Stat,2003(1):858-869.
[13]Bucher C G,Bourgund U.A fast and efficient response surface approach for structural reliability problems[J].Structural Safety,1990,7(1):57-66.
[14]田静萍,郭海英.精油疗法的源流和进展[J].中国调味品,2004(2):2-5.
[15]王广要,周虎,曾晓峰.植物精油应用研究进展[J].食品科技,2006,31(5):11-14.
[16]Burits M,Bucar F.Antioxidant activity of Nigella sativa essential oil[J].Phytotherapy Research,2000,14(5):323-328.
[17]Carson C F,Riley T V.Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia[J].Journal of Applied Bacteriology,1995,78(3):264-269.
[18]赵兵,王玉春,欧阳藩,等.超声波在植物提取中的应用[J].中草药,1999,30(9):1-3.
[19]严伟,李淑芬.超声波协助提取技术[J].化工进展,2002,21(9):649-651.
[20]Lianfu Z,Zelong L.Optimization and comparison of ultrasound/microwave assisted extraction(UMAE)and ultrasonic assisted extraction(UAE)of lycopene from tomatoes[J].Ultrasonics Sonochemistry,2008,15(5):731-737.