单因素试验结合Box-Behnken设计-响应面法优化甘草中黄酮类成分的提取工艺
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摘要
目的:优化甘草中黄酮类成分的提取工艺。方法:以芹糖甘草苷、甘草苷、芹糖异甘草苷、芒柄花苷4种黄酮类成分的总提取量为考察指标,以提取溶剂种类(水、乙醇)及其不同体积分数、加入量与提取时间为考察因素,在单因素试验基础上,运用Box-Behnken设计-响应面法优化甘草中黄酮类成分的提取工艺,并进行验证试验。结果:优化的提取工艺为以50%乙醇为提取溶剂,在0.200 g药材中加入50 mL,超声提取50 min。在验证试验中,芹糖甘草苷、甘草苷、芹糖异甘草苷、芒柄花苷提取量分别为10.733 0、27.784 9、3.441 9、0.429 1 mg/g(RSD均<3.0%,n=3),4种黄酮类成分平均总提取量为42.388 9 mg/g,与预测值(42.173 2mg/g)的相对误差为0.52%(n=3)。结论:优化的提取工艺简便、快捷、稳定,可用于甘草中黄酮类成分的提取。
OBJECTIVE:To optimize the extraction technology of the flavonoids from Glycyrrhiza uralensis. METHODS:Using total contents of four flavonoids,liquiritinapioside,glycyrrhizin,isoliquiritin apioside and formononetin as indexes,typesand volume fractions of extraction solvents(water,ethanol),volume of addition and extraction time as factors,based on singlefactor experiment,Box-Behnken design-response surface method was used to optimize the extraction technology of flavonoids fromG. uralensis. Validation test was also conducted. RESULTS:The optimal extraction technology was 50 mL 50% ethanol asextraction solvent, 0.200 g G. uralensis, ultrasonic extraction for 50 min. In validation test, the extraction amounts ofliquiritinapioside, glycyrrhizin, isoliquiritin apioside and formononetin were 10.733 0,27.784 9,3.441 9,0.429 1 mg/g,respectively(all RSDs<3.0%,n=3). The average total extraction amount of four flavonoids was obtained was 42.388 9 mg/g,the relative error of which to predicted value(42.173 2 mg/g) was 0.52%(n=3). CONCLUSIONS:The optimized extractiontechnology is simple,rapid and stable,and can be used for the extraction of flavonoids from G. uralensis.
OBJECTIVE:To optimize the extraction technology of the flavonoids from Glycyrrhiza uralensis. METHODS:Using total contents of four flavonoids,liquiritinapioside,glycyrrhizin,isoliquiritin apioside and formononetin as indexes,typesand volume fractions of extraction solvents(water,ethanol),volume of addition and extraction time as factors,based on singlefactor experiment,Box-Behnken design-response surface method was used to optimize the extraction technology of flavonoids fromG. uralensis. Validation test was also conducted. RESULTS:The optimal extraction technology was 50 mL 50% ethanol asextraction solvent, 0.200 g G. uralensis, ultrasonic extraction for 50 min. In validation test, the extraction amounts ofliquiritinapioside, glycyrrhizin, isoliquiritin apioside and formononetin were 10.733 0,27.784 9,3.441 9,0.429 1 mg/g,respectively(all RSDs<3.0%,n=3). The average total extraction amount of four flavonoids was obtained was 42.388 9 mg/g,the relative error of which to predicted value(42.173 2 mg/g) was 0.52%(n=3). CONCLUSIONS:The optimized extractiontechnology is simple,rapid and stable,and can be used for the extraction of flavonoids from G. uralensis.
引文
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[14]程艳刚,谭金燕,叶文冲,等.基于Plackett-Burman设计和Box-Behnken响应面法优化仙鹤草总黄酮超声提取工艺及其抗氧化抗肿瘤活性研究[J].中华中医药学刊,2018,36(10):2414-2419.
[15]苏艳莹,雷小小,桂卉,等.星点设计-效应面法优化山茱萸总环烯醚萜苷脂质体的制备工艺[J].中国药房,2018,29(19):2612-2616.
[16]邢国秀,李楠,王童,等.甘草中黄酮类化学成分的研究进展[J].中国中药杂志,2003,28(7):10-14.
[2]王忠全,丁旧玲.复方甘草酸苷的临床应用[J].中国药房,2015,16(20):1585-1586.
[3]高雪岩,王文全,魏胜利,等.甘草及其活性成分的药理活性研究进展[J].中国中药杂志,2009,34(21):2695-2700.
[4]向诚,乔雪,叶敏,等.利用数据库对甘草属植物化学成分的分类和分布分析[J].药学学报,2012,47(8):1023-1030.
[5]方鉴.甘草及其有效成分对免疫系统调节作用探究[J].海峡药学,2017,29(8):28-30.
[6]苏国林,刘刚,刘育辰,等.甘草苷的提取纯化方法和药理作用研究进展[J].中国现代中药,2011,13(10):48-51.
[7]马玲.甘草黄酮及皂苷类成分谱效关系研究[D].银川:宁夏医科大学,2015.
[8]刘育辰,李铮,马丽端,等.同时检测甘草中4种黄酮糖苷类成分的方法及其在基源植物鉴别上的应用[J].中国药学杂志,2011,46(14):1112-1116.
[9]王欢,黄嫣,李希,等.响应面法优化白花蛇舌草中齐墩果酸和熊果酸的提取工艺[J].中国实验方剂学杂志,2015,21(20):34-37.
[10]万丹娜,饶倩如,俞梦莹,等.Box-Behnken设计-响应面法优化山楂的醇沉工艺[J].中国药房,2018,29(15):2078-2081.
[11]张琳,赵凤平,王云红.Box-Behnken响应面法优选枳壳饮片炮制工艺研究[J].亚太传统医药,2018,14(2):61-64.
[12]高雪梅,祁岳,张志.响应面法优化酸藤子叶总黄酮的超声提取工艺[J].黑龙江畜牧兽医,2018(17):167-170,240.
[13]牟会荣,陈昆,王晓岚,等.正交试验法与响应面法在桑叶黄酮提取工艺优化中的应用和比较[J].江苏科技大学学报(自然科学版),2016,30(1):88-93.
[14]程艳刚,谭金燕,叶文冲,等.基于Plackett-Burman设计和Box-Behnken响应面法优化仙鹤草总黄酮超声提取工艺及其抗氧化抗肿瘤活性研究[J].中华中医药学刊,2018,36(10):2414-2419.
[15]苏艳莹,雷小小,桂卉,等.星点设计-效应面法优化山茱萸总环烯醚萜苷脂质体的制备工艺[J].中国药房,2018,29(19):2612-2616.
[16]邢国秀,李楠,王童,等.甘草中黄酮类化学成分的研究进展[J].中国中药杂志,2003,28(7):10-14.