Box-Behnken设计-响应面法优选雪松松针中蛇床子素的提取工艺
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摘要
目的:优选雪松松针中蛇床子素的提取工艺。方法:采用高效液相色谱法测定蛇床子素含量。在单因素考察的基础上,以乙醇体积分数、提取时间、料液比为考察因素,以蛇床子素含量为响应值,采用Box-Behnken设计-响应面法优选雪松松针中蛇床子素的提取工艺,并进行验证试验。结果:确定的最佳提取工艺为乙醇体积分数88%,料液比1∶20(g/mL),提取2次、每次57min。在此工艺条件下,提取得到蛇床子素的平均含量为0.675 7 mg/g(RSD=1.78%,n=3),与预测值0.680 9 mg/g的相对误差为0.59%。结论:优选的最佳提取工艺方法简便、可行,可用于雪松松针中蛇床子素的提取。
OBJECTIVE: To optimize the extraction technology of osthole from the pine needles of Cedrus deodara.METHODS:HPLC method was adopted to determine the content of osthole. Based on single factor test,ethanol volume fraction,extraction time and material-solvent ratio were selected as influential factors,and the content of osthole was selected as responsevalue. Box-Behnken design-response surface methodology was used to optimize the extraction technology of osthole in pine needlesof C. deodara. Validation test was conducted. RESULTS:The optimal extraction technology was as follows as ethanol volumefraction of 88%,material-solvent ratio of 1∶20(g/mL),extracting for 2 times,lasting for 57 min each time. Under thistechnology,average content of osthole was 0.675 7 mg/g(RSD=1.78%,n=3),and the relative error of which to predicted value0.680 9 mg/g was 0.59%. CONCLUSIONS:The optimal extraction technology is simple and feasible,and it can be used for theextraction of osthole from the pine needles of C. deodara.
OBJECTIVE: To optimize the extraction technology of osthole from the pine needles of Cedrus deodara.METHODS:HPLC method was adopted to determine the content of osthole. Based on single factor test,ethanol volume fraction,extraction time and material-solvent ratio were selected as influential factors,and the content of osthole was selected as responsevalue. Box-Behnken design-response surface methodology was used to optimize the extraction technology of osthole in pine needlesof C. deodara. Validation test was conducted. RESULTS:The optimal extraction technology was as follows as ethanol volumefraction of 88%,material-solvent ratio of 1∶20(g/mL),extracting for 2 times,lasting for 57 min each time. Under thistechnology,average content of osthole was 0.675 7 mg/g(RSD=1.78%,n=3),and the relative error of which to predicted value0.680 9 mg/g was 0.59%. CONCLUSIONS:The optimal extraction technology is simple and feasible,and it can be used for theextraction of osthole from the pine needles of C. deodara.
引文
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[3]刘东彦,胡鹏斌,石晓峰,等. HPLC法测定雪松不同部位4个黄酮类化合物的含量[J].中国现代应用药学,2014,31(12):1510-1514.
[4]沈薇,石晓峰,胡彩香,等. HPLC法测定雪松不同部位5种酚酸类成分的含量[J].药物分析杂志,2016,36(12):2174-2179.
[5]沈薇,石晓峰,宁红霞,等.雪松不同部位中原花青素的含量测定及其清除DPPH自由基的活性[J].广西植物,2015,35(6):930-934.
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[7]覃小燕,胡珍,张花美,等.蛇床子素药理作用及相关机制研究进展[J].天津中医药,2018,35(11):877-880.
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[10]何继祥,程静,袁建平.反相高效液相色谱法测定舒洁洗液中蛇床子素含量[J].中成药,2002,24(8):629-630.
[11]张荣,方庆.蛇床子素提取制备工艺的研究[J].中医药学刊,2003,21(8):1336.
[12]赵军,张婷婷,杨蒙蒙,等. HPLC法同时测定连丹乳膏中蛇床子素和丹皮酚的含量[J].中国药房,2014,25(35):3300-3302.
[13]孟繁浩,李遇伯,杨正毅,等. HPLC法同时测定蛇床子中蛇床子素和欧前胡素含量[J].沈阳药科大学学报,2004,21(3):201-204.
[14]王永辉,房树标,高丽,等.高效液相色谱法测定蛇床子素微乳中蛇床子素的含量[J].中华中医药杂志,2013,28(11):3426-3428.
[2]李师,刘东彦,石晓峰,等.雪松松针正丁醇部位的化学成分研究[J].中草药,2014,45(18):2602-2606.
[3]刘东彦,胡鹏斌,石晓峰,等. HPLC法测定雪松不同部位4个黄酮类化合物的含量[J].中国现代应用药学,2014,31(12):1510-1514.
[4]沈薇,石晓峰,胡彩香,等. HPLC法测定雪松不同部位5种酚酸类成分的含量[J].药物分析杂志,2016,36(12):2174-2179.
[5]沈薇,石晓峰,宁红霞,等.雪松不同部位中原花青素的含量测定及其清除DPPH自由基的活性[J].广西植物,2015,35(6):930-934.
[6]石晓峰,李师,刘东彦,等. RP-HPLC法同时测定雪松松针中4个有效成分的含量[J].药物分析杂志,2014,34(2):243-246.
[7]覃小燕,胡珍,张花美,等.蛇床子素药理作用及相关机制研究进展[J].天津中医药,2018,35(11):877-880.
[8]安芳,王书华,张丹参,等. HPLC法测定兔血浆中蛇床子素的含量[J].中草药,2004,35(1):43-44.
[9]孙晓丹.高效液相色谱法测定妇炎平栓蛇床子素的含量[J].现代医药卫生,2010,26(2):165-166,322.
[10]何继祥,程静,袁建平.反相高效液相色谱法测定舒洁洗液中蛇床子素含量[J].中成药,2002,24(8):629-630.
[11]张荣,方庆.蛇床子素提取制备工艺的研究[J].中医药学刊,2003,21(8):1336.
[12]赵军,张婷婷,杨蒙蒙,等. HPLC法同时测定连丹乳膏中蛇床子素和丹皮酚的含量[J].中国药房,2014,25(35):3300-3302.
[13]孟繁浩,李遇伯,杨正毅,等. HPLC法同时测定蛇床子中蛇床子素和欧前胡素含量[J].沈阳药科大学学报,2004,21(3):201-204.
[14]王永辉,房树标,高丽,等.高效液相色谱法测定蛇床子素微乳中蛇床子素的含量[J].中华中医药杂志,2013,28(11):3426-3428.