桔小实蝇视叶结构特征及5-羟色胺能神经元的分布
|
摘要
桔小实蝇是重要的果蔬害虫,它对不同颜色的光表现出不同的趋性。为了明确其视觉感受的结构基础,本研究采用免疫组织化学染色技术结合激光共聚焦成像分析了桔小实蝇成虫视叶内神经髓结构组成和体积大小,并利用5-羟色胺(5-hydroxytryptamine,5-HT)抗体标记了视叶内5-羟色胺能神经元,研究了其在视叶内的分布特征及细胞体数量。结果表明,桔小实蝇成虫的视叶由视神经节层、视髓、副视髓、视小叶和视小叶板5个神经髓结构组成,其中雌成虫的视髓相对体积极显著的大于雄虫的视髓相对体积。桔小实蝇每个视叶中包含12个5-HT能神经元细胞体,位于视髓的腹内侧,副视髓的前方。视叶5个神经髓区均含5-HT能神经纤维,但它们的神经纤维来自不同的神经元。对视叶神经髓结构及5-HT能神经元分布特征的研究将为未来构建桔小实蝇视觉神经通路和阐明5-HT对视觉感受的调控机制奠定解剖学基础。
The oriental fruit fly Bactrocera dorsalis Hendel is an important pest of fruits and vegetables, it shows a different tendency to the light of different colors. In order to clarify the structural basis of visual perception, the structure and volume of optic lobes in adult flies were analyzed by immunohistochemical staining combined with laser confocal imaging. The serotoninergic neurons in the optic lobe were labeled with 5-HT antibody, and the distribution characteristics and the number of somata in the optic lobes were studied. The results showed that the optic lobes of adults flies were composed of the lamina, medulla, accessory medulla, lobula,and lobula plate. The relative volume of the medulla of the female was significantly larger than that of the male adults. Each optic lobe contains 12 5-HT immunoreactivity cell bodies, which mainly distribute in the ventromedial part of the medulla and anterior of the accessory medulla. All five neuropils in the optic lobe contain 5-HT immunoreactivity neural processes, but they innervated by different neurons. The study on the structure of the neuropils in the optic lobe and the distribution of 5-HT immunoreactivity neurons will lay the anatomical foundation for constructing the visual neural pathway and clarifying the mechanism of the regulation of serotonin on the visual perception of oriental fruit fly in the future.
The oriental fruit fly Bactrocera dorsalis Hendel is an important pest of fruits and vegetables, it shows a different tendency to the light of different colors. In order to clarify the structural basis of visual perception, the structure and volume of optic lobes in adult flies were analyzed by immunohistochemical staining combined with laser confocal imaging. The serotoninergic neurons in the optic lobe were labeled with 5-HT antibody, and the distribution characteristics and the number of somata in the optic lobes were studied. The results showed that the optic lobes of adults flies were composed of the lamina, medulla, accessory medulla, lobula,and lobula plate. The relative volume of the medulla of the female was significantly larger than that of the male adults. Each optic lobe contains 12 5-HT immunoreactivity cell bodies, which mainly distribute in the ventromedial part of the medulla and anterior of the accessory medulla. All five neuropils in the optic lobe contain 5-HT immunoreactivity neural processes, but they innervated by different neurons. The study on the structure of the neuropils in the optic lobe and the distribution of 5-HT immunoreactivity neurons will lay the anatomical foundation for constructing the visual neural pathway and clarifying the mechanism of the regulation of serotonin on the visual perception of oriental fruit fly in the future.
引文
Chen XY,Liu JL,Guo WJ,et al.Associative olfactory conditioning in male adults of the oriental fruit fly,Bactrocera dorsalis (Diptera:Tephritidae) [J].Acta Entomologica Sinica,2015,58 (9):973-980.[陈小燕,刘家莉,郭文举,等.桔小实蝇雄成虫联系性嗅觉学习 [J].昆虫学报,2015,58 (9):973-980]
El Jundi B,Huetteroth W,Kurylas AE,et al.Anisometric brain dimorphism revisited:Implementation of a volumetric 3D standard brain in Manduca sexta [J].Journal of Comparative Neurology,2009,517 (2):210-225.
Fischbach KF,Dittrich APM.The optic lobe of Drosophila melanogaster.I.A Golgi analysis of wild-type structure [J].Cell and Tissue Research,1989,258 (3):441-475.
Guo P,Ma BW,Zhao XC,et al.Distribution of 5-hydroxytryptamine in the optic lobes of the oriental armyworm,Mythimna separata (Lepidotpera:Noctuidae) [J].Acta Entomologica Sinica,2018,61 (6):668-675.[郭培,马百伟,赵新成,等.5-羟色胺在粘虫视叶中的分布 [J].昆虫学报,2018,61 (6):668-675]
Hansson BS,Christensen TA,Hildebrand JG.Functionally distinct subdivisions of the macroglomerular complex in the antennal lobe of the male sphinx moth Manduca sexta [J].Journal of Comparative Neurology,1991,312 (2):264-278.
Heinze S,Reppert SM.Anatomical basis of sun compass navigation I:The general layout of the monarch butterfly brain [J].Journal of Comparative Neurology,2012,520 (8):1599-1628.
Homberg U,Christensen TA,Hildebrand JG.Structure and function of the deutocerebrum in insects [J].Annual Review of Entomology,1989,34 (1):477-501.
Immonen EV,Dacke M,Heinze S,et al.Anatomical organization of the brain of a diurnal and a nocturnal dung beetle [J].Journal of Comparative Neurology,2017,525 (8):1879-1908.
Ito K,Shinomiya K,Ito M,et al.A systematic nomenclature for the insect brain [J].Neuron,2014,81 (4):755-765.
Kinoshita M,Pfeiffer K,Homberg U.Spectral properties of identified polarized-light sensitive interneurons in the brain of the desert locust Schistocerca gregaria [J].Journal of Experimental Biology,2007,210 (8):1350-1361.
Kloppenburg P,Erber J.The modulatory effects of serotonin and octopamine in the visual system of the honey bee (Apis mellifera L.) II.Electrophysiological analysis of motion-sensitive neurons in the lobula [J].Journal of Comparative Physiology A,1995,176 (1):119-129.
Kurylas AE,Rohlfing T,Krofczik S,et al.Standardized atlas of the brain of the desert locust,Schistocerca gregaria [J].Cell and Tissue Research,2008,333 (1):125.
Leitinger G,Pabst MA,Kral K.Serotonin-immunoreactive neurons in the visual system of the praying mantis:An immunohistochemical,confocal laser scanning and electron microscopic study [J].Brain Research,1999,823 (1-2):11-23.
Liang DH,Chen R,Zhou Q.Responsiveness of Bactrocera dorsalis (Hendel) adult to different colors and odorants [J].Life Science Research,2016,20 (1):36-39.[梁丹辉,陈冉,周琼.桔小实蝇成虫对不同颜色和气味物质的趋性反应 [J].生命科学研究,2016,20 (1):36-39]
Liu H,Gao Z,Deng SZ,et al.The photokinesis of oriental fruit flies,Bactrocera dorsalis,to LED lights of various wavelengths [J].Entomologia Experimentalis et Applicata,2018,166 (2):102-112.
Liu JH,Zhao ZH.Roles of insect vision in host plant finding and locating [J].Journal of Plant Protection,2017,44 (3):353-362.[刘军和,赵紫华.昆虫视觉在寄主寻找及定位过程中的作用 [J].植物保护学报,2017,44 (3):353-362]
Mancini N,Giurfa M,Sandoz JC,et al.Aminergic neuromodulation of associative visual learning in harnessed honey bees [J].Neurobiology of Learning and Memory,2018,155:556-567.
Morante J,Desplan C.Seminars in Cell and Developmental Biology:Building a Projection Map for Photoreceptor Neurons in the Drosophila optic Lobes [C] Academic Press,2004,15 (1):137-143.
N?ssel DR,Klemm N.Serotonin-like immunoreactivity in the optic lobes of three insect species [J].Cell and Tissue Research,1983,232 (1):129-140.
Nériec N,Desplan C.Current Topics in Developmental Biology:From the Eye to the Brain:Development of the Drosophila Visual System [M].Academic Press,2016,116:247-271.
Niu H,Li Y,Mao X.Distribution of GABA and 5-HT-ergic immunoreactive neurons in the visual system of butterfly Mimathyma schrenckii [J].Acta Zoologica Sinica,2004,50 (5):770-777.
Ott SR.Confocal microscopy in large insect brains:Zinc-formaldehyde fixation improves synapsin immunostaining and preservation of morphology in whole-mounts [J].Journal of Neuroscience Methods,2008,172 (2):220-230.
Piňero JC,Souder SK,Vargas RI.Vision-mediated exploitation of a novel host plant by a tephritid fruit fly [J].PLoS ONE,2017,12 (4):e0174636.
Qi YX,Wu SF,Huang J,et al.Advances in 5-hydroxytryptamine and its receptors in insects [J].Acta Entomologica Sinica,2014,57 (7):844-859.[齐易香,吴顺凡,黄佳,等.昆虫5-羟色胺及其受体的研究进展 [J].昆虫学报,2014,57 (7):844-859]
Reeves JL.Vision should not be overlooked as an important sensory modality for finding host plants [J].Environmental Entomology,2011,40 (4):855-863.
Rosner R,von Hadeln J,Salden T,et al.Anatomy of the lobula complex in the brain of the praying mantis compared to the lobula complexes of the locust and cockroach [J].Journal of Comparative Neurology,2017,525 (10):2343-2357.
Schürmann FW,Klemm N.Serotonin-immunoreactive neurons in the brain of the honeybee [J].Journal of Comparative Neurology,1984,225 (4):570-580.
Settembrini BP,Villar MJ.Distribution of serotonin in the central nervous system of the blood-feeding heteropteran,Triatoma infestans (Heteroptera:Reduviidae) [J].Journal of Morphology,2004,260 (1):21-32.
Sizemore TR,Dacks AM.Serotonergic modulation differentially targets distinct network elements within the antennal lobe of Drosophila melanogaster [J].Scientific Reports,2016,6:37119.
Strausfeld NJ,Blest AD.Golgi studies on insects Part I.The optic lobes of Lepidoptera [J].Philosophical Transactions of the Royal Society of London B,Biological Sciences,1970,258 (820):81-134.
Strausfeld NJ.Golgi studies on insects Part II.The optic lobes of Diptera [J].Philosophical Transactions of the Royal Society of London.B,Biological Sciences,1970,258 (820):135-223.
Tsuji E,Aonuma H,Yokohari F,et al.Serotonin-immunoreactive neurons in the antennal sensory system of the brain in the carpenter ant,Camponotus japonicus [J].Zoological Science,2007,24 (8):836-850.
Vallés AM,White K.Serotonin-containing neurons in Drosophila melanogaster:Development and distribution [J].Journal of Comparative Neurology,1988,268 (3):414-428.
Vleugels R,Verlinden H,Vanden Broeck J.Serotonin,serotonin receptors and their actions in insects [J].Neurotransmitter,2015,2:1-14.
Vogt K,Aso Y,Hige T,et al.Direct neural pathways convey distinct visual information to Drosophila mushroom bodies [J].Elife,2016,5:e14009.
Wei HY,Jundi B,Homberg U,et al.Implementation of pigment-dispersing factor-immunoreactive neurons in a standardized atlas of the brain of the cockroach Leucophaea maderae [J].Journal of Comparative Neurology,2010,518 (20):4113-4133.
Williamson WR,Hiesinger PR.Preparation of developing and adult Drosophila brains and retinae for live imaging [J].Journal of Visualized Experiments,2010,37:e1936.
El Jundi B,Huetteroth W,Kurylas AE,et al.Anisometric brain dimorphism revisited:Implementation of a volumetric 3D standard brain in Manduca sexta [J].Journal of Comparative Neurology,2009,517 (2):210-225.
Fischbach KF,Dittrich APM.The optic lobe of Drosophila melanogaster.I.A Golgi analysis of wild-type structure [J].Cell and Tissue Research,1989,258 (3):441-475.
Guo P,Ma BW,Zhao XC,et al.Distribution of 5-hydroxytryptamine in the optic lobes of the oriental armyworm,Mythimna separata (Lepidotpera:Noctuidae) [J].Acta Entomologica Sinica,2018,61 (6):668-675.[郭培,马百伟,赵新成,等.5-羟色胺在粘虫视叶中的分布 [J].昆虫学报,2018,61 (6):668-675]
Hansson BS,Christensen TA,Hildebrand JG.Functionally distinct subdivisions of the macroglomerular complex in the antennal lobe of the male sphinx moth Manduca sexta [J].Journal of Comparative Neurology,1991,312 (2):264-278.
Heinze S,Reppert SM.Anatomical basis of sun compass navigation I:The general layout of the monarch butterfly brain [J].Journal of Comparative Neurology,2012,520 (8):1599-1628.
Homberg U,Christensen TA,Hildebrand JG.Structure and function of the deutocerebrum in insects [J].Annual Review of Entomology,1989,34 (1):477-501.
Immonen EV,Dacke M,Heinze S,et al.Anatomical organization of the brain of a diurnal and a nocturnal dung beetle [J].Journal of Comparative Neurology,2017,525 (8):1879-1908.
Ito K,Shinomiya K,Ito M,et al.A systematic nomenclature for the insect brain [J].Neuron,2014,81 (4):755-765.
Kinoshita M,Pfeiffer K,Homberg U.Spectral properties of identified polarized-light sensitive interneurons in the brain of the desert locust Schistocerca gregaria [J].Journal of Experimental Biology,2007,210 (8):1350-1361.
Kloppenburg P,Erber J.The modulatory effects of serotonin and octopamine in the visual system of the honey bee (Apis mellifera L.) II.Electrophysiological analysis of motion-sensitive neurons in the lobula [J].Journal of Comparative Physiology A,1995,176 (1):119-129.
Kurylas AE,Rohlfing T,Krofczik S,et al.Standardized atlas of the brain of the desert locust,Schistocerca gregaria [J].Cell and Tissue Research,2008,333 (1):125.
Leitinger G,Pabst MA,Kral K.Serotonin-immunoreactive neurons in the visual system of the praying mantis:An immunohistochemical,confocal laser scanning and electron microscopic study [J].Brain Research,1999,823 (1-2):11-23.
Liang DH,Chen R,Zhou Q.Responsiveness of Bactrocera dorsalis (Hendel) adult to different colors and odorants [J].Life Science Research,2016,20 (1):36-39.[梁丹辉,陈冉,周琼.桔小实蝇成虫对不同颜色和气味物质的趋性反应 [J].生命科学研究,2016,20 (1):36-39]
Liu H,Gao Z,Deng SZ,et al.The photokinesis of oriental fruit flies,Bactrocera dorsalis,to LED lights of various wavelengths [J].Entomologia Experimentalis et Applicata,2018,166 (2):102-112.
Liu JH,Zhao ZH.Roles of insect vision in host plant finding and locating [J].Journal of Plant Protection,2017,44 (3):353-362.[刘军和,赵紫华.昆虫视觉在寄主寻找及定位过程中的作用 [J].植物保护学报,2017,44 (3):353-362]
Mancini N,Giurfa M,Sandoz JC,et al.Aminergic neuromodulation of associative visual learning in harnessed honey bees [J].Neurobiology of Learning and Memory,2018,155:556-567.
Morante J,Desplan C.Seminars in Cell and Developmental Biology:Building a Projection Map for Photoreceptor Neurons in the Drosophila optic Lobes [C] Academic Press,2004,15 (1):137-143.
N?ssel DR,Klemm N.Serotonin-like immunoreactivity in the optic lobes of three insect species [J].Cell and Tissue Research,1983,232 (1):129-140.
Nériec N,Desplan C.Current Topics in Developmental Biology:From the Eye to the Brain:Development of the Drosophila Visual System [M].Academic Press,2016,116:247-271.
Niu H,Li Y,Mao X.Distribution of GABA and 5-HT-ergic immunoreactive neurons in the visual system of butterfly Mimathyma schrenckii [J].Acta Zoologica Sinica,2004,50 (5):770-777.
Ott SR.Confocal microscopy in large insect brains:Zinc-formaldehyde fixation improves synapsin immunostaining and preservation of morphology in whole-mounts [J].Journal of Neuroscience Methods,2008,172 (2):220-230.
Piňero JC,Souder SK,Vargas RI.Vision-mediated exploitation of a novel host plant by a tephritid fruit fly [J].PLoS ONE,2017,12 (4):e0174636.
Qi YX,Wu SF,Huang J,et al.Advances in 5-hydroxytryptamine and its receptors in insects [J].Acta Entomologica Sinica,2014,57 (7):844-859.[齐易香,吴顺凡,黄佳,等.昆虫5-羟色胺及其受体的研究进展 [J].昆虫学报,2014,57 (7):844-859]
Reeves JL.Vision should not be overlooked as an important sensory modality for finding host plants [J].Environmental Entomology,2011,40 (4):855-863.
Rosner R,von Hadeln J,Salden T,et al.Anatomy of the lobula complex in the brain of the praying mantis compared to the lobula complexes of the locust and cockroach [J].Journal of Comparative Neurology,2017,525 (10):2343-2357.
Schürmann FW,Klemm N.Serotonin-immunoreactive neurons in the brain of the honeybee [J].Journal of Comparative Neurology,1984,225 (4):570-580.
Settembrini BP,Villar MJ.Distribution of serotonin in the central nervous system of the blood-feeding heteropteran,Triatoma infestans (Heteroptera:Reduviidae) [J].Journal of Morphology,2004,260 (1):21-32.
Sizemore TR,Dacks AM.Serotonergic modulation differentially targets distinct network elements within the antennal lobe of Drosophila melanogaster [J].Scientific Reports,2016,6:37119.
Strausfeld NJ,Blest AD.Golgi studies on insects Part I.The optic lobes of Lepidoptera [J].Philosophical Transactions of the Royal Society of London B,Biological Sciences,1970,258 (820):81-134.
Strausfeld NJ.Golgi studies on insects Part II.The optic lobes of Diptera [J].Philosophical Transactions of the Royal Society of London.B,Biological Sciences,1970,258 (820):135-223.
Tsuji E,Aonuma H,Yokohari F,et al.Serotonin-immunoreactive neurons in the antennal sensory system of the brain in the carpenter ant,Camponotus japonicus [J].Zoological Science,2007,24 (8):836-850.
Vallés AM,White K.Serotonin-containing neurons in Drosophila melanogaster:Development and distribution [J].Journal of Comparative Neurology,1988,268 (3):414-428.
Vleugels R,Verlinden H,Vanden Broeck J.Serotonin,serotonin receptors and their actions in insects [J].Neurotransmitter,2015,2:1-14.
Vogt K,Aso Y,Hige T,et al.Direct neural pathways convey distinct visual information to Drosophila mushroom bodies [J].Elife,2016,5:e14009.
Wei HY,Jundi B,Homberg U,et al.Implementation of pigment-dispersing factor-immunoreactive neurons in a standardized atlas of the brain of the cockroach Leucophaea maderae [J].Journal of Comparative Neurology,2010,518 (20):4113-4133.
Williamson WR,Hiesinger PR.Preparation of developing and adult Drosophila brains and retinae for live imaging [J].Journal of Visualized Experiments,2010,37:e1936.