Beta介孔沸石负载CHCA用于MALDI-TOF-MS分析恩诺沙星小分子化合物的初探
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摘要
考察了Beta沸石材料负载传统有机基质α-氰基-4-羟基肉桂酸(CHCA)应用于基质辅助激光解吸电离-飞行时间质谱(MALDI-TOF-MS)分析恩诺沙星药物小分子的效果。实验中,分别制备了质子、钠离子及银离子交换的Beta沸石,并在相同质谱条件下进行了比较研究,考察了不同离子交换的Beta沸石以及CHCA/Beta Na不同混合比例对恩诺沙星药物小分子的检测效果。实验结果表明,使用金属离子交换的Beta沸石作为基质材料,能够实现恩诺沙星药物小分子的选择性离子化,同时,Beta Na沸石负载CHCA新型复合基质用于恩诺沙星药物小分子的分析中,具有较理想的抑制干扰碎片离子、提高恩诺沙星质谱峰强度等效果。此外,结合前期研究认为,沸石表面活性位点与恩诺沙星药物小分子存在相互作用关系,进而影响MALDI-TOF-MS质谱分析的离子化效率。
Cyano-4-hydroxycinnamic acid( CHCA) loading on Beta zeolite as novel composite matrix was applied in matrix assisted laser desorption ionization time-of-flight mass spectrometry( MALDI-TOF-MS) analysis of enrofloxacin small compounds.In the experiments,H+,Na+and Ag+ion exchanged Beta zeolites were prepared,respectively.These new matrixes were examined and compared in the analysis of enrofloxacin at the same MALDI-TOF-MS conditions.The results indicated that the enrofloxacin could be selectively ionized by using metal ions exchanged zeolite.Additionally,CHCA loading on Beta Na zeolite with the mass ratio of4 ∶2 was capable of suppressing fragments and promoting peak intensity of enrofloxacin. Based on previous research,it was presumed that the interaction between the activity site on zeolite surface and enrofloxacin would affect the ionization efficiency of MALDI-TOF-MS analysis.
Cyano-4-hydroxycinnamic acid( CHCA) loading on Beta zeolite as novel composite matrix was applied in matrix assisted laser desorption ionization time-of-flight mass spectrometry( MALDI-TOF-MS) analysis of enrofloxacin small compounds.In the experiments,H+,Na+and Ag+ion exchanged Beta zeolites were prepared,respectively.These new matrixes were examined and compared in the analysis of enrofloxacin at the same MALDI-TOF-MS conditions.The results indicated that the enrofloxacin could be selectively ionized by using metal ions exchanged zeolite.Additionally,CHCA loading on Beta Na zeolite with the mass ratio of4 ∶2 was capable of suppressing fragments and promoting peak intensity of enrofloxacin. Based on previous research,it was presumed that the interaction between the activity site on zeolite surface and enrofloxacin would affect the ionization efficiency of MALDI-TOF-MS analysis.
引文
[1]TANAKA K,WAKI H,IDO Y,et al.Disulfide bond formation has occurred correctly and rapid commun[J].Mass Spectrom.,1988,2:151-153.
[2]DONG X,CHENG J,LI J,et al.Graphene as a novel matrix for the analysis of small molecules by MALDI-TOF MS[J].Anal.Chem.,2010,82(14):6 208-6 214.
[3]WANG P,GIESE R W.Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry[J]. J. Chromatogr.A,2017,1 486:35-41.
[4]YANG M,FUJINO T.Silver nanoparticles on zeolite surface for laser desorption/ionization mass spectrometry of low molecular weight compounds[J]. Chem. Phys. Lett.,2013,576:61-64.
[5]LU M,YANG X,YANG Y,et al.Nanomaterials as assisted matrix of laser desorption/ionization time-of-flight mass spectrometry for the analysis of small molecules[J].Nanomaterials,2017,7(4):87.
[6]LI X H,WU X,KIM J M,et al.MALDI-TOF-MS analysis of small molecules using modified mesoporous material SBA-15 as assisted matrix[J].J.Am.Soc.Mass Spectrom.,2009,20(11):2 167-2 173.
[7]TANG H Z,MA Y L,LIU F,et al.Detection of small molecules using SBA-15 modified CHCA as a novel matrix of MALDI-TOF MS[J].Int.J.Mass Spectrom.,2017,417:34-39.
[8]MIN Q,ZHANG X,CHEN X,et al.N-doped graphene:an alternative carbon-based matrix for highly efficient detection of small molecules by negative ion MALDI-TOF MS[J].Anal.Chem.,2014,86(18):9 122-9 130.
[9]LIN Z,ZHENG J,LIN G,et al.Negative ion laser desorption/ionization time-of-flight mass spectrometric analysis of small molecules using graphitic carbon nitride nanosheet matrix[J].Anal.Chem.,2015,87(15):8 005-8 012.
[10]董美花,孙士美,金彪.氧化硅介孔材料在基质辅助激光解析飞行时间质谱分析有机小分子中的应用[J].分析化学,2014,42(9):1 235-1 239.
[11]WANG S,NIU H,ZENG T,et al.Rapid determination of small molecule pollutants using metal-organic frameworks as adsorbent and matrix of MALDI-TOF-MS[J].Microporous Mesoporous Mat.,2017,239:390-395.
[12]CAI Z,YAN X P,YANG X,et al. Zeolitic imidazolate framework nanocrystals for enrichment and direct detection of environmental pollutants by negative ion surfaceassisted laser desorption/ionization time-of-flight mass spectrometry[J]. RSC Adv.,2016,6(28):23 790-23 793.
[13]SUZUKI J,FUJINO T.Matrix-assisted laser desorption ionization mass spectrometry of maltohexaose and acetylsalicylic acid using alkali metal cation-substituted zeolites[J].Anal.Sci.,2012,28(9):901-904.
[14]SUZUKI J,SATO A,YAMAMOTO R,et al.Matrix-assisted laser desorption ionization using lithium-substituted mordenite surface[J]. Chem. Phys. Lett.,2012,546(3):159-163.
[15]刘婧,杨武,毛学荣,等.磺酸基功能化的介孔材料HSO-3-Zr-La-SBA-15的制备[J].化学试剂,2018,40(5):483-486.
[16]杨梦瑞,李鹏,王敏,等.介孔沸石材料负载传统基质用于激光解吸电离-飞行时间质谱分析小分子化合物[J].分析化学,2017,45(10):1 542-1 546.
[17]MENGRUI Y,FUJINO T.Silver nanoparticles on zeolite surface for laser desorption/ionization mass spectrometry of low molecular weight compounds[J]. Chem. Phys.Lett.,2013,576:61-64.
[18]KOMORI Y,SHIMA H,FUJINO T,et al.Pronounced selectivity in matrix-assisted laser desorption-ionization mass spectrometry with 2,4,6-trihydroxyacetophenone on a zeolite surface:intensity enhancement of protonated peptides and suppression of matrix-related ions[J]. J.Phys.Chem.,2010,114(3):1 593-1 600.
[19]YAMAMOTO R,FUJINO T.2,4,6-Trihydroxyacetophenone on zeolite surface:correlation between electronic relaxation and fragmentation on mass spectra[J].Chem.Phys.Lett.,2012,543:76-81.
[2]DONG X,CHENG J,LI J,et al.Graphene as a novel matrix for the analysis of small molecules by MALDI-TOF MS[J].Anal.Chem.,2010,82(14):6 208-6 214.
[3]WANG P,GIESE R W.Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry[J]. J. Chromatogr.A,2017,1 486:35-41.
[4]YANG M,FUJINO T.Silver nanoparticles on zeolite surface for laser desorption/ionization mass spectrometry of low molecular weight compounds[J]. Chem. Phys. Lett.,2013,576:61-64.
[5]LU M,YANG X,YANG Y,et al.Nanomaterials as assisted matrix of laser desorption/ionization time-of-flight mass spectrometry for the analysis of small molecules[J].Nanomaterials,2017,7(4):87.
[6]LI X H,WU X,KIM J M,et al.MALDI-TOF-MS analysis of small molecules using modified mesoporous material SBA-15 as assisted matrix[J].J.Am.Soc.Mass Spectrom.,2009,20(11):2 167-2 173.
[7]TANG H Z,MA Y L,LIU F,et al.Detection of small molecules using SBA-15 modified CHCA as a novel matrix of MALDI-TOF MS[J].Int.J.Mass Spectrom.,2017,417:34-39.
[8]MIN Q,ZHANG X,CHEN X,et al.N-doped graphene:an alternative carbon-based matrix for highly efficient detection of small molecules by negative ion MALDI-TOF MS[J].Anal.Chem.,2014,86(18):9 122-9 130.
[9]LIN Z,ZHENG J,LIN G,et al.Negative ion laser desorption/ionization time-of-flight mass spectrometric analysis of small molecules using graphitic carbon nitride nanosheet matrix[J].Anal.Chem.,2015,87(15):8 005-8 012.
[10]董美花,孙士美,金彪.氧化硅介孔材料在基质辅助激光解析飞行时间质谱分析有机小分子中的应用[J].分析化学,2014,42(9):1 235-1 239.
[11]WANG S,NIU H,ZENG T,et al.Rapid determination of small molecule pollutants using metal-organic frameworks as adsorbent and matrix of MALDI-TOF-MS[J].Microporous Mesoporous Mat.,2017,239:390-395.
[12]CAI Z,YAN X P,YANG X,et al. Zeolitic imidazolate framework nanocrystals for enrichment and direct detection of environmental pollutants by negative ion surfaceassisted laser desorption/ionization time-of-flight mass spectrometry[J]. RSC Adv.,2016,6(28):23 790-23 793.
[13]SUZUKI J,FUJINO T.Matrix-assisted laser desorption ionization mass spectrometry of maltohexaose and acetylsalicylic acid using alkali metal cation-substituted zeolites[J].Anal.Sci.,2012,28(9):901-904.
[14]SUZUKI J,SATO A,YAMAMOTO R,et al.Matrix-assisted laser desorption ionization using lithium-substituted mordenite surface[J]. Chem. Phys. Lett.,2012,546(3):159-163.
[15]刘婧,杨武,毛学荣,等.磺酸基功能化的介孔材料HSO-3-Zr-La-SBA-15的制备[J].化学试剂,2018,40(5):483-486.
[16]杨梦瑞,李鹏,王敏,等.介孔沸石材料负载传统基质用于激光解吸电离-飞行时间质谱分析小分子化合物[J].分析化学,2017,45(10):1 542-1 546.
[17]MENGRUI Y,FUJINO T.Silver nanoparticles on zeolite surface for laser desorption/ionization mass spectrometry of low molecular weight compounds[J]. Chem. Phys.Lett.,2013,576:61-64.
[18]KOMORI Y,SHIMA H,FUJINO T,et al.Pronounced selectivity in matrix-assisted laser desorption-ionization mass spectrometry with 2,4,6-trihydroxyacetophenone on a zeolite surface:intensity enhancement of protonated peptides and suppression of matrix-related ions[J]. J.Phys.Chem.,2010,114(3):1 593-1 600.
[19]YAMAMOTO R,FUJINO T.2,4,6-Trihydroxyacetophenone on zeolite surface:correlation between electronic relaxation and fragmentation on mass spectra[J].Chem.Phys.Lett.,2012,543:76-81.