摘要
考察了Beta沸石材料负载传统有机基质α-氰基-4-羟基肉桂酸(CHCA)应用于基质辅助激光解吸电离-飞行时间质谱(MALDI-TOF-MS)分析恩诺沙星药物小分子的效果。实验中,分别制备了质子、钠离子及银离子交换的Beta沸石,并在相同质谱条件下进行了比较研究,考察了不同离子交换的Beta沸石以及CHCA/Beta Na不同混合比例对恩诺沙星药物小分子的检测效果。实验结果表明,使用金属离子交换的Beta沸石作为基质材料,能够实现恩诺沙星药物小分子的选择性离子化,同时,Beta Na沸石负载CHCA新型复合基质用于恩诺沙星药物小分子的分析中,具有较理想的抑制干扰碎片离子、提高恩诺沙星质谱峰强度等效果。此外,结合前期研究认为,沸石表面活性位点与恩诺沙星药物小分子存在相互作用关系,进而影响MALDI-TOF-MS质谱分析的离子化效率。
Cyano-4-hydroxycinnamic acid( CHCA) loading on Beta zeolite as novel composite matrix was applied in matrix assisted laser desorption ionization time-of-flight mass spectrometry( MALDI-TOF-MS) analysis of enrofloxacin small compounds.In the experiments,H+,Na+and Ag+ion exchanged Beta zeolites were prepared,respectively.These new matrixes were examined and compared in the analysis of enrofloxacin at the same MALDI-TOF-MS conditions.The results indicated that the enrofloxacin could be selectively ionized by using metal ions exchanged zeolite.Additionally,CHCA loading on Beta Na zeolite with the mass ratio of4 ∶2 was capable of suppressing fragments and promoting peak intensity of enrofloxacin. Based on previous research,it was presumed that the interaction between the activity site on zeolite surface and enrofloxacin would affect the ionization efficiency of MALDI-TOF-MS analysis.
引文
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