Box-Behnken响应面法优化排闼宗阳颗粒提取工艺
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摘要
目的:采用Box-Behnken响应面法筛选出排闼宗阳颗粒最佳提取工艺。方法:通过建立一种HPLC法能够同时测定排闼宗阳颗粒中绿原酸、咖啡酸、槲皮苷三种指标成分,并以其含量为指标,在单因素试验基础上,采用Box-Behnken响应面法考察提取时间、加水倍数、提取次数对三种指标成分的影响。结果:采用Design-Expert 8. 06软件对所得数据进行处理,并通过软件对三个模型进行整合,得到的最佳提取工艺为提取次数2次,提取时间1. 08 h,加水倍量8. 6倍。结论:该HPLC方法准确、重复性良好,Box-Behnken优化提取工艺的方法稳定可行,操作简单,可为深入研究及应用提供依据。
Objective: To screen the best extraction technology of Paita Zongyang granule by Bbox-Behnken response surface methodology. Methods: An HPLC method was established to simultaneously determine chlorogenic acid,caffeic acid and quercitrin in Paita Zongyang granule. Based on single factor experiments,Box-Behnken response surface methodology was used to investigate the effects of extraction time,water addition times and extraction times on the three index components. Results: The data were processed by design-expert 8. 06 software,and three models were integrated by the software. The optimal extraction process was twice extraction,1. 08 h extraction time and 8. 6 times of water addition. Conclusion: The HPLC method is accurate and repeatable. The extraction process optimized by Box-Behnken method is stable,feasible and simple to operate,which can provide basis for the further research and application.
Objective: To screen the best extraction technology of Paita Zongyang granule by Bbox-Behnken response surface methodology. Methods: An HPLC method was established to simultaneously determine chlorogenic acid,caffeic acid and quercitrin in Paita Zongyang granule. Based on single factor experiments,Box-Behnken response surface methodology was used to investigate the effects of extraction time,water addition times and extraction times on the three index components. Results: The data were processed by design-expert 8. 06 software,and three models were integrated by the software. The optimal extraction process was twice extraction,1. 08 h extraction time and 8. 6 times of water addition. Conclusion: The HPLC method is accurate and repeatable. The extraction process optimized by Box-Behnken method is stable,feasible and simple to operate,which can provide basis for the further research and application.
引文
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10江迎春,邓翀,李景丽.Box-Behnken响应面法优选金樱子提取工艺[J].中国中医药信息杂志,2015,22(8):99-102
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13席晓志,王婉卿,崔晓伟,等.响应面优化杜仲提取物抗氧化活性及其工艺研究[J].中国现代中药,2018,20(3):310-315
2 Fuentes E,Caballero J.Chlorogenic Acid Inhibits Human Platelet Activation and Thrombus Formation[J].Plos One,2014,9(3):1-13
3王旭瑞,常亮.浅谈蒙医药用植物成分绿原酸抗氧化作用[J].中国民族医药杂志,2016,10(10):44-45
4 Kumaran Ks,Prince PSM.Caffeic acid protects rat heart mitochondria against isoproterenol-induced oxidative damage[J].Cell Stress and Chaperones,2010,15:791-806
5林鹏飞,贾小舟,祁燕,等.酚酸类化合物研究进展[J].广东化工,2017,1(44):50-52
6汤小平,马哲龙,施宁川,等.槲皮苷衍生物的合成及药理作用研究[J].中国药师,2016,19(2):251-254
7石宏志,高南南,李勇枝,等.泽兰有效部分对血小板聚集和血栓形成的影响[J].中草药,2003,34(10):923-926
8张红兵,韩梅,温进坤.欧亚旋覆花总黄酮类提取物抑制内皮损伤诱导的血管氧化应激反应[J].中国中药杂志,2009,34(5):615-619
9周迎春,郭丽新,王世龙.泽兰有效成分对急性血瘀大鼠凝血功能和体外血栓形成的影响[J].中医药学报,2013,41(1):22-24
10江迎春,邓翀,李景丽.Box-Behnken响应面法优选金樱子提取工艺[J].中国中医药信息杂志,2015,22(8):99-102
11陈哲,路娟,彭纪铭,等.Box-Behnken设计-效应面法优化紫檀茋纳米混悬剂处方[J].中医药信息,2017,34(6):14-19
12傅光辉,湛建峰.Box-Behnken效应面法优化决明子中蒽醌类成分提取工艺[J].中国药师,2017,20(9):1554-1558
13席晓志,王婉卿,崔晓伟,等.响应面优化杜仲提取物抗氧化活性及其工艺研究[J].中国现代中药,2018,20(3):310-315