Bt基因导入对海岛棉农艺性状和产量性状影响的研究
|
摘要
对9个经PCR检测确定为转Bt基因阳性的海岛棉品系的农艺性状和产量性状考察结果表明,外源基因Bt的导入对9个海岛棉品系的农艺性状和产量性状有不同程度的影响。1受体为K222时,Bt基因导入使得株高、始节高、始节数、有效果枝数、铃数、有效铃数差异达到极显著水平(P<0.01)。当受体为XH25和XH30时,转基因品系农艺性状与对照差异无显著水平;2 9个转基因品系间株高、始节高、始节数和铃数差异达到极显著水平(P<0.01),有效果枝数和有效铃数差异达到显著水平(P<0.05);3 9个转基因品系的产量性状与对照差异均无显著性;4株高、始节高、始节数、果枝数、有效果枝数、铃数和有效铃数与籽棉产量呈显著相关;5两年(2014和2015)和两点(北疆和南疆)间农艺性状及产量性状差异达到显著水平。
The agronomic and yield characters of nine strains of Gossypium barbadense L.,in which Bt gene was detected by PCR,were studied. The results showed that agronomic characters and yield characters were impacted to different extent.(1) When K222 was the recipient,the difference in plant height,start high,initial segment number,number of effective branches,boll number and number of effective bolls got to excellent significant level(P < 0.01). When XH25 and XH30 were recipients,there was no difference between transgenic and non-transgenic;(2)The difference in plant height,start high,initial segment number and boll number between the nine strains got to excellent significant level(P < 0. 01). Difference in number of effective branches and number of effective boll arrived at significant level(P < 0. 05);(3) There was no significant difference between transgenic and non-transgenic in yield characters;(4) Plant height,start high,initial segment number,boll stem number,number of effective branches,boll number and number of effective boll were significantly related to yield;(5) The difference between two years(2014 and 2015) and two areas(the northern of Xinjiang and the southern of Xinjiang) got to significant level in agronomic characters and yield characters.
The agronomic and yield characters of nine strains of Gossypium barbadense L.,in which Bt gene was detected by PCR,were studied. The results showed that agronomic characters and yield characters were impacted to different extent.(1) When K222 was the recipient,the difference in plant height,start high,initial segment number,number of effective branches,boll number and number of effective bolls got to excellent significant level(P < 0.01). When XH25 and XH30 were recipients,there was no difference between transgenic and non-transgenic;(2)The difference in plant height,start high,initial segment number and boll number between the nine strains got to excellent significant level(P < 0. 01). Difference in number of effective branches and number of effective boll arrived at significant level(P < 0. 05);(3) There was no significant difference between transgenic and non-transgenic in yield characters;(4) Plant height,start high,initial segment number,boll stem number,number of effective branches,boll number and number of effective boll were significantly related to yield;(5) The difference between two years(2014 and 2015) and two areas(the northern of Xinjiang and the southern of Xinjiang) got to significant level in agronomic characters and yield characters.
引文
[1]何龙飞,韦善清,卢升安,等.转SCK基因水稻后代农艺性状变异的研究[J].广西农业生物科学,2002,21(2):73-77.
[2]Tabashnik B E,Van Rensburg.Field-evolved insect resistance to bt crops:definition,theory,and data[J].Journal of Economic Entomology,2009,102(6):2011-2025.
[3]Lu YH,Wu KM,Jiang YY,et al.Mirid bug outbreaks in multiple crops correlated with wide-scale adoption of Bt cotton in chin[J].Science,2010,328(5982):1151-1154.
[4]张欣,付亚萍,周君莉,等.水稻规模化转基因技术体系构建与应用[J].中国农业科学,2014,47(21):4141-4154.
[5]Flores S,Saxena D,Stotzky G.Transgenic Bt plants decompose less in soil than non-Bt plants[J].Soil Biology&Biochemistry,2005,37(6):1073-1082.
[6]喻树迅.我国棉花生产现状与发展趋势[J].中国工程科学,2013,15(4):9-13.
[7]张锐,王远,孟志刚,等.国产转基因抗虫棉研究回顾与展望[J].中国农业科技导报,2007,9(4):32-42.
[8]秦永华,乔志新,刘进元.转基因技术在棉花育种上的应用[J].棉花学报,2007,19(6):482-488.
[9]郑巨云,李雪源,王俊铎,等.新疆转基因棉花育种展望[J].中国棉花,2010,37(11):2-5.
[10]王孝纲,别墅,张教海,等.我国转基因棉花育种概况与展望[J].湖北农业科学,2003,(3):32-35.
[11]Tabashnik BE,Carriere Y,Dennehy TJ,et al.Insect resistance to transgenic Bt crops:Lessons from the laboratory and field[J].Journal of Economic Entomology,2003,96(4):1031-1038.
[12]Romeis J,Meissle M,Bigler F.Transgenic crops expressing Bacillus thuringiensis toxins and biological control[J].Nature Biotechnology,2006,24(1):63-71.
[13]丰嵘,张宝红,郭香墨.外源Bt基因对棉花产量性状及抗虫性的影响[J].棉花学报,1996,8(1):10-13.
[14]张香桂,周宝良,陈松,等.抗虫棉与常规棉主要农艺及经济性状的比较研究[J].中国棉花,2004,31(5):14-16.
[15]刘冬梅,武芝霞,刘传亮,等.花粉管通道法获得棉花转基因株系主要农艺性状变异分析[J].棉花学报,2007,19(6):450-454.
[16]刘方,王坤波,宋国立,等.棉花转基因材料的获得及主要农艺性状变异分析[J].棉花学报,2009,21(1):23-27.
[17]刘剑光,肖松华,狄佳春,等.Bt基因导入对棉花农艺性状的影响[J].中国棉花,2003,30(3):15-17.
[18]刘慧君,简桂良,邹亚飞.GO基因导入对棉花农艺性状及抗病性的影响[J].分子植物育种,2003,1(5/6);669-672.
[19]钱进,杨云尧,任燕萍,等.转Mv NHX1基因棉花抗旱性研究和株系变异分析[J].分子植物育种,2014,12(1):80-86.
[20]连丽君,吕素莲,李汝忠,等.转Bet A/als基因棉花材料的农艺性状考察[J].棉花学报,2008,20(6):447-451.
[21]崔海瑞,王忠华,舒庆尧,等.转Bt基因水稻克螟稻杂交转育后代农艺性状的研究[J].中国水稻科学,2001,15(2):22-27.
[22]唐祚舜,李良材,田文忠.基因枪法转基因水稻后代农艺性状的表现[J].中国农业科学,2001,34(6):581-586.
[23]莫春红.转Bt基因水稻Bt蛋白表达量检测及螟虫抗性和农艺性状评价[D].武汉:华中农业大学,2014.
[24]徐泽俊,聂以春,张献龙,等.转双价抗虫基因棉花的主要农艺性状的遗传变异[J].植物遗传资源学报,2011,12(1):125-130.
[25]吕淑平,郭小平,赵元明.转基因抗虫棉Bt基因导入对受体材料农艺性状的影响[J].中国农学通报,2004,20(3):36-37.
[26]王忠华,张立成.Bt抗虫转基因水稻的育种应用[J].核农学报,2007,21(2):148-151,200.
[27]陈文岳.转Bt基因水稻“克螟稻”的遗传改良研究[D].杭州:浙江大学,2005.
[28]王彩霞,吴殿星,沈圣泉,等.转基因及常规水稻幼苗对潮霉素敏感性的研究[J].核农学报,2005,19(3):168-171.
[29]沈圣泉,舒庆尧,包劲松,等.应用近等基因系研究Bt基因对水稻性状表现的影响[J].浙江大学学报(农业与生命科学版),2005,31(3):283-287.
[30]刘昌文,张燕,宋义前.新疆早熟棉花主要农艺性状相关性及多项式趋势分析[J].天津农业科学,2008,14(3):11-16.
[31]周桂生,周福才,谢义明,等.温度胁迫对转Bt基因抗虫棉毒蛋白的表达和棉铃虫死亡率的影响[J].棉花学报,2009,21(4):302-306.
[32]沈平,林克剑,张永军,等.转Bt基因棉不同品种杀虫蛋白季节性表达及其对棉铃虫的控制作用[J].棉花学报,2010,22(5):393-397.
[2]Tabashnik B E,Van Rensburg.Field-evolved insect resistance to bt crops:definition,theory,and data[J].Journal of Economic Entomology,2009,102(6):2011-2025.
[3]Lu YH,Wu KM,Jiang YY,et al.Mirid bug outbreaks in multiple crops correlated with wide-scale adoption of Bt cotton in chin[J].Science,2010,328(5982):1151-1154.
[4]张欣,付亚萍,周君莉,等.水稻规模化转基因技术体系构建与应用[J].中国农业科学,2014,47(21):4141-4154.
[5]Flores S,Saxena D,Stotzky G.Transgenic Bt plants decompose less in soil than non-Bt plants[J].Soil Biology&Biochemistry,2005,37(6):1073-1082.
[6]喻树迅.我国棉花生产现状与发展趋势[J].中国工程科学,2013,15(4):9-13.
[7]张锐,王远,孟志刚,等.国产转基因抗虫棉研究回顾与展望[J].中国农业科技导报,2007,9(4):32-42.
[8]秦永华,乔志新,刘进元.转基因技术在棉花育种上的应用[J].棉花学报,2007,19(6):482-488.
[9]郑巨云,李雪源,王俊铎,等.新疆转基因棉花育种展望[J].中国棉花,2010,37(11):2-5.
[10]王孝纲,别墅,张教海,等.我国转基因棉花育种概况与展望[J].湖北农业科学,2003,(3):32-35.
[11]Tabashnik BE,Carriere Y,Dennehy TJ,et al.Insect resistance to transgenic Bt crops:Lessons from the laboratory and field[J].Journal of Economic Entomology,2003,96(4):1031-1038.
[12]Romeis J,Meissle M,Bigler F.Transgenic crops expressing Bacillus thuringiensis toxins and biological control[J].Nature Biotechnology,2006,24(1):63-71.
[13]丰嵘,张宝红,郭香墨.外源Bt基因对棉花产量性状及抗虫性的影响[J].棉花学报,1996,8(1):10-13.
[14]张香桂,周宝良,陈松,等.抗虫棉与常规棉主要农艺及经济性状的比较研究[J].中国棉花,2004,31(5):14-16.
[15]刘冬梅,武芝霞,刘传亮,等.花粉管通道法获得棉花转基因株系主要农艺性状变异分析[J].棉花学报,2007,19(6):450-454.
[16]刘方,王坤波,宋国立,等.棉花转基因材料的获得及主要农艺性状变异分析[J].棉花学报,2009,21(1):23-27.
[17]刘剑光,肖松华,狄佳春,等.Bt基因导入对棉花农艺性状的影响[J].中国棉花,2003,30(3):15-17.
[18]刘慧君,简桂良,邹亚飞.GO基因导入对棉花农艺性状及抗病性的影响[J].分子植物育种,2003,1(5/6);669-672.
[19]钱进,杨云尧,任燕萍,等.转Mv NHX1基因棉花抗旱性研究和株系变异分析[J].分子植物育种,2014,12(1):80-86.
[20]连丽君,吕素莲,李汝忠,等.转Bet A/als基因棉花材料的农艺性状考察[J].棉花学报,2008,20(6):447-451.
[21]崔海瑞,王忠华,舒庆尧,等.转Bt基因水稻克螟稻杂交转育后代农艺性状的研究[J].中国水稻科学,2001,15(2):22-27.
[22]唐祚舜,李良材,田文忠.基因枪法转基因水稻后代农艺性状的表现[J].中国农业科学,2001,34(6):581-586.
[23]莫春红.转Bt基因水稻Bt蛋白表达量检测及螟虫抗性和农艺性状评价[D].武汉:华中农业大学,2014.
[24]徐泽俊,聂以春,张献龙,等.转双价抗虫基因棉花的主要农艺性状的遗传变异[J].植物遗传资源学报,2011,12(1):125-130.
[25]吕淑平,郭小平,赵元明.转基因抗虫棉Bt基因导入对受体材料农艺性状的影响[J].中国农学通报,2004,20(3):36-37.
[26]王忠华,张立成.Bt抗虫转基因水稻的育种应用[J].核农学报,2007,21(2):148-151,200.
[27]陈文岳.转Bt基因水稻“克螟稻”的遗传改良研究[D].杭州:浙江大学,2005.
[28]王彩霞,吴殿星,沈圣泉,等.转基因及常规水稻幼苗对潮霉素敏感性的研究[J].核农学报,2005,19(3):168-171.
[29]沈圣泉,舒庆尧,包劲松,等.应用近等基因系研究Bt基因对水稻性状表现的影响[J].浙江大学学报(农业与生命科学版),2005,31(3):283-287.
[30]刘昌文,张燕,宋义前.新疆早熟棉花主要农艺性状相关性及多项式趋势分析[J].天津农业科学,2008,14(3):11-16.
[31]周桂生,周福才,谢义明,等.温度胁迫对转Bt基因抗虫棉毒蛋白的表达和棉铃虫死亡率的影响[J].棉花学报,2009,21(4):302-306.
[32]沈平,林克剑,张永军,等.转Bt基因棉不同品种杀虫蛋白季节性表达及其对棉铃虫的控制作用[J].棉花学报,2010,22(5):393-397.