转Bt基因抗虫棉外源Bt蛋白的差异表达
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摘要
【目的】研究转Bt基因抗虫棉外源Bt蛋白表达的时空规律,明确Bt蛋白表达量和转Bt基因抗虫棉抗性的内在联系,更加有效降低靶标害虫危害。【方法】采用ELISA(Enzyme-linked immunosorbent assay)蛋白定量检测方法,以转Bt基因抗虫棉鄂抗虫棉1号(GK19)为研究材料,对转Bt基因抗虫棉不同组织器官、不同生长时期主茎功能叶和不同生长时期主茎不同叶位叶片的Bt蛋白含量进行检测。【结果】转Bt基因抗虫棉不同组织器官Bt蛋白含量存在差异,苗期叶片最高,花、蕾、铃次之,根、茎较低;不同生长时期主茎功能叶Bt蛋白含量呈现出随生长发育时间推进而降低的趋势;不同生长时期主茎不同叶位叶片Bt蛋白含量差异较大,苗期和盛蕾期第1~7叶呈现出逐渐下降的趋势,而盛花期和盛铃期第1~7叶呈现出先缓慢升高后逐渐稳定的趋势。【结论】Bt蛋白在转Bt基因抗虫棉各生长时期,各组织器官都有表达,且表达量呈时空动态变化。
【Objective】Studying on the temporal and spatial dynamics of Bt protein expression in Bt transgenic cotton and determining the relation of Bt protein expression and Bt transgenic cotton can effectively reduce the harm of target pests.【Method】In this paper,the Bt protein contents in the different tissues,organs,main stem functional leaves and main stem leaves at different positions of transgenic cotton cultivar( GKl9) in different growth stages were studied. 【Result】The Bt protein contents of all tissues and organs were different,which were in order of seedling > flower > boll > bud > root > stem. There was a reduction tendency of Bt protein in main stem functional leaves in different growth stages with the advance of development. The Bt protein contents among main stem leaves at different positions in different growth stages were obviously different,which indicated the gradual decrease from the first to the seventh leaves in seedling and full budding stage and a gradual stabilization after a slow increase from the first to the seventh leaves in full flowering and bolling stages. 【Conclusion】The Bt protein in all growth periods,all tissues and organs of Bt transgenic cotton were expressed,which changed with temporal and spatial dynamics.
【Objective】Studying on the temporal and spatial dynamics of Bt protein expression in Bt transgenic cotton and determining the relation of Bt protein expression and Bt transgenic cotton can effectively reduce the harm of target pests.【Method】In this paper,the Bt protein contents in the different tissues,organs,main stem functional leaves and main stem leaves at different positions of transgenic cotton cultivar( GKl9) in different growth stages were studied. 【Result】The Bt protein contents of all tissues and organs were different,which were in order of seedling > flower > boll > bud > root > stem. There was a reduction tendency of Bt protein in main stem functional leaves in different growth stages with the advance of development. The Bt protein contents among main stem leaves at different positions in different growth stages were obviously different,which indicated the gradual decrease from the first to the seventh leaves in seedling and full budding stage and a gradual stabilization after a slow increase from the first to the seventh leaves in full flowering and bolling stages. 【Conclusion】The Bt protein in all growth periods,all tissues and organs of Bt transgenic cotton were expressed,which changed with temporal and spatial dynamics.
引文
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[10]Mazier M,Chaufaux J,Sanchis V,et al.The cryic gene from Bacillus thuringiensis provides protection against Spodoptera littoralis in young transgenic plants[J].Plant Science,1997,127:179-190.
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[2]孙伟,曹玉洪.转Bt基因抗虫棉Bt毒蛋白表达量的时空变化[J].安徽农业科学,2005,33(2):202-203.
[3]李汝忠,沈法富,王宗文,等.转Bt基因抗虫棉Bt基因表达的时空动态[J].山东农业科学,2002(2):7-9.
[4]姜周庚,张延昭,冯梦诗,等.高温胁迫对盛铃期Bt棉棉铃中杀虫蛋白表达量及氮代谢的影响[J].中国棉花,2012,39(4):13-32.
[5]张祥,王桂霞,顾超,等.高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响[J].应用生态学报,2012,23(11):3016-3020.
[6]王家宝,王留明,沈法富,等.环境因素对转Bt基因棉Bt杀虫蛋白表达量的影响[J].山东农业科学,2000(6):4-6.
[7]张桂玲,温四民.盐胁迫对转Bt基因棉苗期Bt蛋白表达量和氮代谢的影响[J].西北农业学报,2011,20(6):106-109.
[8]代建龙,董合忠,段留生,等.施氮肥对盐胁迫下Bt棉生长和叶片Bt蛋白含量的影响[J].棉花学报,2012,24(4):303-311.
[9]Narvaez-Vasquez J,Orozca-Cardenas M L,Ryan C A.Differential expression of a chimeric Ca MV-tomato proteinase Inhibitor I gene in leaves of transformed nightshade,tobacco and alfalfa plants[J].Plant Molecular Biology,1992,20:1149-1157.
[10]Mazier M,Chaufaux J,Sanchis V,et al.The cryic gene from Bacillus thuringiensis provides protection against Spodoptera littoralis in young transgenic plants[J].Plant Science,1997,127:179-190.
[11]Pauk J,Stefanov I,Fekete S,et al.A study of different(Ca MV35S and mas)promoter activities and risk assessment of field use in transgenic rapeseed plants[J].Euphytica,1995,85:411-416.