黄刺玫果提取物对小鼠急性酒精性氧化应激反应的影响
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摘要
目的探讨黄刺玫果提取物对小鼠急性酒精氧化应激损伤的抗氧化作用。方法将小鼠随机分为5组,分别为空白对照组,模型组,黄刺玫果提取物低、中、高剂量组,采用50%乙醇经口灌胃造模,测定血清和肝组织中超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、丙二醛(MDA)、蛋白质羰基含量。结果乙醇氧化模型造模成功,中剂量组、大剂量组小鼠血清中SOD、GSH、MDA、蛋白质羰基与模型组比较,差异均有统计学意义(均P<0.05);中剂量组、大剂量组小鼠肝组织中SOD、GSH、MDA、蛋白质羰基与模型组比较,差异均有统计学意义(均P<0.05)。结论黄刺玫果提取物对小鼠急性酒精性氧化应激反应具有抗氧化作用,为黄刺玫果开发功能性食品提供实验依据。
Objective To study the antioxidant effect of Rosa xanthina Lindl fruit extract on acute alcoholic oxidative stress reaction mice. Methods Mice were randomly divided into 5 groups: blank control group, model group, low, medium and high dose of Rose xanthina Lindl fruit extract groups. The model was established by intragastric administration of 50% ethanol.Determined the contents of superoxide dismutase(SOD), reduced glutathione(GSH), malondialdehyde(MDA) and protein carbonyl in serum and liver tissue. Results The model of ethanol oxidation was successfully established. Compared with the model group, the differences of SOD, GSH, MDA and protein carbonyl in serum of medium-dose group and high-dose group had statistical significance(P<0.05). Compared with the model group, the differences of SOD, GSH, MDA and protein carbonyl in liver tissue of medium-dose group and high-dose group had statistical significance(P<0.05). Conclusion Rose xanthina Lindl fruit extract has antioxidant effect on acute alcoholic oxidative stress reaction in mice, which provides experimental basis for the development of functional food using Rose xanthina Lindl fruit.
Objective To study the antioxidant effect of Rosa xanthina Lindl fruit extract on acute alcoholic oxidative stress reaction mice. Methods Mice were randomly divided into 5 groups: blank control group, model group, low, medium and high dose of Rose xanthina Lindl fruit extract groups. The model was established by intragastric administration of 50% ethanol.Determined the contents of superoxide dismutase(SOD), reduced glutathione(GSH), malondialdehyde(MDA) and protein carbonyl in serum and liver tissue. Results The model of ethanol oxidation was successfully established. Compared with the model group, the differences of SOD, GSH, MDA and protein carbonyl in serum of medium-dose group and high-dose group had statistical significance(P<0.05). Compared with the model group, the differences of SOD, GSH, MDA and protein carbonyl in liver tissue of medium-dose group and high-dose group had statistical significance(P<0.05). Conclusion Rose xanthina Lindl fruit extract has antioxidant effect on acute alcoholic oxidative stress reaction in mice, which provides experimental basis for the development of functional food using Rose xanthina Lindl fruit.
引文
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[13]刘同刚,沙凯辉,王邦茂.三七对小鼠急性酒精性肝损伤保护作用的实验研究[J].山东医药, 2010, 50(16):44-45.
[14]文静,张春华,董甫,等.蛋白质羰基含量与蛋白质氧化损伤[J].食品科学, 2003, 24(10):153-157.
[2] Loguercio C, Federico A. Oxidative stress in viral and alcoholic hepatitis[J]. Free Radic BiolMed, 2003, 34(1):1-10.
[3]山西植物志编委会.山西植物志[M].北京:中国科学技术出版, 1998.
[4]高阳,姜会敏,霍雅玉,等.刺玫果的体外抗氧化活性研究[J].西北药学志, 2014, 29(3):226-230.
[5]焦淑萍,陈彪,姜红.山刺玫果实清除羟自由基及抗DNA损伤作用的实验研究[J].北华大学学报(自然科学版), 2002, 3(4):307-308, 318.
[6]焦淑萍,杨春玫,初秋,等.山刺玫果降血脂、抗氧化及保护血管内皮功能的实验研究[J].北华大学学报(自然科学版), 2005,6(3):228-230.
[7]郝龙平,晏嘉泽,靳艳,等.刺玫果多糖的制备及其抗氧化活性[J].中成药, 2016, 38(12):2692-2696.
[8]朱峰,岳红,祖恩峰,等.新型功能材料杜仲胶的研究与应用[J].安徽大学学报(自然科学版), 2005, 29(3):89-94.
[9]杨李,唐瑛,左娟,等.硫代巴比妥酸法测定血清脂质过氧化物方法的改进[J].华南国防医学杂志, 2004, 18(1):30-32.
[10] Li X. Improved pyrogallol autoxidation method:a reliable and cheap superoxide-scavengingassay suitable for all antioxidants[J]. J Agric Food Chem, 2012, 60(25):6418-6424.
[11]江正辉,王泰龄.酒精性肝病[M].北京:中国医药科技出版社, 2001.
[12]童英,姚小曼,吴少平.乙醇诱发急性肝损伤生物标记物的探讨[J].中国食品卫生杂志, 1999, 11(2):12-14.
[13]刘同刚,沙凯辉,王邦茂.三七对小鼠急性酒精性肝损伤保护作用的实验研究[J].山东医药, 2010, 50(16):44-45.
[14]文静,张春华,董甫,等.蛋白质羰基含量与蛋白质氧化损伤[J].食品科学, 2003, 24(10):153-157.