短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用研究
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摘要
目的:探讨短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用。方法:分别用不同浓度的乙酸钠、丙酸钠和丁酸钠孵育C2C12细胞24 h,MTS试验检测细胞活力,Western blot检测AMPK磷酸化水平。结果:与对照组相比,1 mmol/L和4 mmol/L乙酸钠,4 mmol/L丙酸钠以及4 mmol/L、8 mmol/L和16 mmol/L丁酸钠升高AMPK磷酸化水平,但不影响其总蛋白水平。结论:短链脂肪酸激活C2C12小鼠骨骼肌细胞AMPK。
Objective: To investigate the effect of SCFAs on AMPK in mouse C2 C12 skeletal muscle cells. Methods: C2 C12 cells were incubated with different concentrations of sodium acetate, sodium propionate and sodium butyrate for 24 hours, respectively. The cell viability was measured by MTS assay. The phosphorylation of AMPK was detected by western blot. Results: Compared with the control group, the phosphorylation of AMPK increased under treatments with 1 mmol/L and 4 mmol/L sodium acetate, 4 mmol/L propionate and4 mmol/L, 8 mmol/L, and 16 mmol/L butyrate, respectively. The total protein level of AMPK was not affected. Conclusion: SCFAs phosphorylate AMPK in mouse C2 C12 skeletal muscle cells.
Objective: To investigate the effect of SCFAs on AMPK in mouse C2 C12 skeletal muscle cells. Methods: C2 C12 cells were incubated with different concentrations of sodium acetate, sodium propionate and sodium butyrate for 24 hours, respectively. The cell viability was measured by MTS assay. The phosphorylation of AMPK was detected by western blot. Results: Compared with the control group, the phosphorylation of AMPK increased under treatments with 1 mmol/L and 4 mmol/L sodium acetate, 4 mmol/L propionate and4 mmol/L, 8 mmol/L, and 16 mmol/L butyrate, respectively. The total protein level of AMPK was not affected. Conclusion: SCFAs phosphorylate AMPK in mouse C2 C12 skeletal muscle cells.
引文
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[2]Bryan K,McGivney B A,Farries G,et al.Equine skeletal muscle adaptations to exercise and training:evidence of differential regulation of autophagosomal and mitochondrial components[J].BMC genomics,2017,18(1):595
[3]Krabbe K S,Nielsen A R,Krogh-Madsen R,et al.Brain-derived neurotrophic factor(BDNF)and type 2 diabetes[J].Diabetologia,2007,50(2):431
[4]Friedrichsen M,Mortensen B,Pehmoller C,et al.Exercise-induced AMPK activity in skeletal muscle:role in glucose uptake and insulin sensitivity[J].Mol cell endocrinol,2013,366(2):204
[5]Xie W,Wang L,Dai Q,et al.Activation of AMPK restricts coxsackievirus B3 replication by inhibiting lipid accumulation[J].Jmol cell cardiol,2015,85:155
[6]Maruvada P,Leone V,Kaplan L M,et al.The human microbiome and obesity:moving beyond associations[J].Cell Host Microbe,2017,22(5):589
[7]Canfora E E,Jocken J W,Blaak E E.Short-chain fatty acids in control of body weight and insulin sensitivity[J].Nat rev Endocrinol,2015,11(10):577
[8]den Besten G,Bleeker A,Gerding A,et al.Short-Chain fatty acids protect against high-fat diet-induced obesity via a PPARgammadependent switch from lipogenesis to fat oxidation[J].Diabetes,2015,64(7):2398
[9]Zhang B B,Zhou G,Li C.AMPK:an emerging drug target for diabetes and the metabolic syndrome[J].Cell metab,2009,9(5):407
[10]廖健文,岳莹莹,牛文彦.AMPK对Rab-GAP的调节作用研究[J].天津医科大学学报,2018,24(2):97
[11]Hardie D G.AMPK-sensing energy while talking to other signaling pathways[J].Cell metab,2014,20(6):939
[12]Lantier L,Fentz J,Mounier R,et al.AMPK controls exercise endurance,mitochondrial oxidative capacity,and skeletal muscle integrity[J].FASEB J,2014,28(7):3211
[13]Lin Y H,Chen Y,Smith T C 2nd,et al.Short-Chain Fatty Acids(SCFAs)alter metabolic and virulence attributes of Borrelia burgdorferi[J].Infect immun,2018,86(9):pii:e00217-18.doi:10.1128/IAI.00217-18.Print 2018 Sep
[14]Andersson U,Branning C,Ahrne S,et al.Probiotics lower plasma glucose in the high-fat fed C57BL/6J mouse[J].Benef microbes,2010,1(2):189
[15]M embrez M,Blancher F,Jaquet M,et al.Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice[J].FASEB J,2008,22(7):2416
[16]Gao Z,Yin J,Zhang J,et al.Butyrate improves insulin sensitivity and increases energy expenditure in mice[J].Diabetes,2009,58(7):1509
[17]Palacios-Gonzalez B,Zarain-Herzberg A,Flores-Galicia I,et al.Genistein stimulates fatty acid oxidation in a leptin receptorindependent manner through the JAK2-mediated phosphorylation and activation of AMPK in skeletal muscle[J].Biochim Biophys Acta,2014,1841(1):132