摘要
目的:探讨短链脂肪酸对C2C12小鼠骨骼肌细胞AMPK的作用。方法:分别用不同浓度的乙酸钠、丙酸钠和丁酸钠孵育C2C12细胞24 h,MTS试验检测细胞活力,Western blot检测AMPK磷酸化水平。结果:与对照组相比,1 mmol/L和4 mmol/L乙酸钠,4 mmol/L丙酸钠以及4 mmol/L、8 mmol/L和16 mmol/L丁酸钠升高AMPK磷酸化水平,但不影响其总蛋白水平。结论:短链脂肪酸激活C2C12小鼠骨骼肌细胞AMPK。
Objective: To investigate the effect of SCFAs on AMPK in mouse C2 C12 skeletal muscle cells. Methods: C2 C12 cells were incubated with different concentrations of sodium acetate, sodium propionate and sodium butyrate for 24 hours, respectively. The cell viability was measured by MTS assay. The phosphorylation of AMPK was detected by western blot. Results: Compared with the control group, the phosphorylation of AMPK increased under treatments with 1 mmol/L and 4 mmol/L sodium acetate, 4 mmol/L propionate and4 mmol/L, 8 mmol/L, and 16 mmol/L butyrate, respectively. The total protein level of AMPK was not affected. Conclusion: SCFAs phosphorylate AMPK in mouse C2 C12 skeletal muscle cells.
引文
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