BALB/c突变卷毛小鼠皮肤创伤愈合对比分析
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摘要
目的探讨BALB/c突变卷毛小鼠在皮肤损伤自然愈合过程中与对照BALB/c小鼠存在的差异。方法选择6周龄BALB/c突变卷毛小鼠和对照BALB/c小鼠各10只,于小鼠背部做2个直径为0.5 cm的圆形皮肤全层创口,备皮肤损伤模型,于损伤后第3,7,10,14天观察皮肤外形愈合情况,测量皮肤损伤面积的变化及计算伤口愈合率;同时于损伤后第3,7,10,14天取损伤部位皮肤连带周围正常皮肤,进行HE染色和天狼猩红染色,以观察皮肤在愈合过程中的形态学变化。结果外观结果显示:突变卷毛小鼠在创后3 d和7d的愈合伤口明显愈合较快。愈合率结果显示:卷毛小鼠的皮肤在创后3 d、7d的愈合率显著高于对照小鼠的愈合率,具有统计学意义(P<0.05),而在创后10 d和14 d两组小鼠的愈合率不具有显著差异。病理结果显示:突变卷毛小鼠在创后3 d创面胶原纤维明显增多,可见少量炎症细胞浸润,毛细血管扩张充血,创后7 d胶原增长迅速,肉芽组织显著增多,可见部分表皮再生生长。天狼猩红染色结果显示:卷毛小鼠在创后3 d至14 d可以清晰的看到胶原纤维和肉芽组织从底层逐渐顶替修复破损部位,直至表皮和毛囊生长出来。结论 BALB/c突变卷毛小鼠皮肤创伤后前期的愈合能力强于对照小鼠,为今后该突变小鼠应用于皮肤创伤愈合模型研究提供了理论参考。
Objective To investigate the difference between BALB/c mutant curly mice and control BALB/c mice in terms of natural healing of skin injuries. Methods Ten 6-week-old BALB/c mutant curly mice and 10 control BALB/c mice were selected. Two full-thickness skin wounds with a diameter of 0.5 cm were created on the back of the mice. A skin injury model was used. Skin healing was observed at 3, 7, 10, and 14 days after injury, and changes in the skin damage area and the wound healing rate were measured. Animals were sacrificed at 3, 7, 10, and 14 days after injury. HE staining and Sirius staining were performed to observe pathological changes in the skin during healing. Results The skin wounds healed faster in the mutant curly mice at 3 and 7 days after wound healing. The skin wound healing rate of the curly mice at 3 and 7 days after injury was significantly higher than that of the control mice(P < 0.05), but there was no significant difference between the two groups at 10 and 14 days after injury. The pathological examination showed that the collagen fibers of the mutant curly haired mice increased significantly 3 days after injury, and there was a small amount of inflammatory cell infiltration, and capillary dilation and congestion. Collagen grew rapidly 7 days after injury, levels of granulation tissue increased significantly, and some epidermis regenerated and grew. The Sirius scarlet staining result showed that collagen fibers and granulation tissue were present from the bottom layer of the injury, to repair the damaged area, until the epidermis and hair follicles grew out from 3 to 14 days in the curly-haired mice. Conclusions BALB/c mutant curly mice have better healing ability than control mice in the early stage of skin trauma, which provides a theoretical reference for the application of BALB/c mutant mice in skin wound healing models in the future.
Objective To investigate the difference between BALB/c mutant curly mice and control BALB/c mice in terms of natural healing of skin injuries. Methods Ten 6-week-old BALB/c mutant curly mice and 10 control BALB/c mice were selected. Two full-thickness skin wounds with a diameter of 0.5 cm were created on the back of the mice. A skin injury model was used. Skin healing was observed at 3, 7, 10, and 14 days after injury, and changes in the skin damage area and the wound healing rate were measured. Animals were sacrificed at 3, 7, 10, and 14 days after injury. HE staining and Sirius staining were performed to observe pathological changes in the skin during healing. Results The skin wounds healed faster in the mutant curly mice at 3 and 7 days after wound healing. The skin wound healing rate of the curly mice at 3 and 7 days after injury was significantly higher than that of the control mice(P < 0.05), but there was no significant difference between the two groups at 10 and 14 days after injury. The pathological examination showed that the collagen fibers of the mutant curly haired mice increased significantly 3 days after injury, and there was a small amount of inflammatory cell infiltration, and capillary dilation and congestion. Collagen grew rapidly 7 days after injury, levels of granulation tissue increased significantly, and some epidermis regenerated and grew. The Sirius scarlet staining result showed that collagen fibers and granulation tissue were present from the bottom layer of the injury, to repair the damaged area, until the epidermis and hair follicles grew out from 3 to 14 days in the curly-haired mice. Conclusions BALB/c mutant curly mice have better healing ability than control mice in the early stage of skin trauma, which provides a theoretical reference for the application of BALB/c mutant mice in skin wound healing models in the future.
引文
[1] 楼敏铭,王宏伟,马洁.脐带间充质干细胞条件培养液联合透明质酸修复小鼠皮肤损伤[J].中国组织工程研究,2018,22(1):20-25.
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[7] 张伟,刘伟江,王鹏,等.间充质干细胞培养上清对小鼠皮肤损伤修复的实验研究[J].军事医学,2018,42(3):176-181.
[8] 赵光明,董建勋,李媛,等.回阳生肌方药对糖尿病皮肤溃疡大鼠创面愈合的影响[J].北京中医药大学学报,2014,37(7):495-499,后插2-后插3.
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[11] 王海涛,朱梓赫,李树伟,等.皮肤创伤愈合过程中短暂扩增细胞增生及皮肤干细胞迁移[J].中国组织化学与细胞化学杂志,2016,25(4):328-334.
[12] 伍小敏,蔡放,王宏法,等.罗哌卡因对皮肤损伤愈合及GM-CSF表达的影响[J].中国现代医学杂志,2017,27(6):23-26.
[13] Ding Y,Cui L,Zhao Q,et al.Platelet-rich fibrin accelerates skin wound healing in diabetic mice[J].Ann Plast Surg,2017,79(3):e15-e19.
[14] 丁越,张辉,孙晓菊.骨髓间充质干细胞促进皮肤创伤愈合及细胞因子在愈合过程表达研究[J].中国实用口腔科杂志,2015,8(2):103-107.
[15] Greaves NS,Ashcroft KJ,Baguneid M,et al.Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing [J].J Dermatol Sci,2013,72(3):206-217.
[16] 李晓娟,李硕,白冰珂,等.BALB/c突变卷毛小鼠皮肤组织结构的分析[J].中国比较医学杂志,2014,24(4):47-50.
[2] Wang PH,Huang BS,Yeh CC,et al.Wound healing[J].J Chin Med Assoc,2018,81(2):94-101.
[3] 朱婷,冯玉,肖静,等.三七皂苷R1不同给药方式对皮肤创伤愈合作用的影响[J].中国药理学通报,2018,34(3):325-330.
[4] 李晓娟,李硕,白冰珂,等.BALB/c突变卷毛小鼠皮肤组织结构的分析[J].中国比较医学杂志,2014,24(4):47-50.
[5] 张凯,司艺玲,林季,等.瘦素及其抗体对小鼠皮肤创伤的修复作用[J].标记免疫分析与临床,2008,15(4):231.
[6] 刘亚敏,张诗武,魏焕萍.免疫组织化学和天狼猩红双重染色技术在病理诊断中的应用[J].武警医学院学报,2004,13(3):237-238.
[7] 张伟,刘伟江,王鹏,等.间充质干细胞培养上清对小鼠皮肤损伤修复的实验研究[J].军事医学,2018,42(3):176-181.
[8] 赵光明,董建勋,李媛,等.回阳生肌方药对糖尿病皮肤溃疡大鼠创面愈合的影响[J].北京中医药大学学报,2014,37(7):495-499,后插2-后插3.
[9] Hong WX,Hu MS,Esquivel M,et al.The role of hypoxia-inducible factor in wound healing[J].Adv Wound Care (New Rochelle),2014,3(5):390-399.
[10] Yanai H,Lumenta DB,Vierlinger K,et al.Middle age has a significant impact on gene expression during skin wound healing in male mice[J].Biogerontology,2016,17(4):763-770.
[11] 王海涛,朱梓赫,李树伟,等.皮肤创伤愈合过程中短暂扩增细胞增生及皮肤干细胞迁移[J].中国组织化学与细胞化学杂志,2016,25(4):328-334.
[12] 伍小敏,蔡放,王宏法,等.罗哌卡因对皮肤损伤愈合及GM-CSF表达的影响[J].中国现代医学杂志,2017,27(6):23-26.
[13] Ding Y,Cui L,Zhao Q,et al.Platelet-rich fibrin accelerates skin wound healing in diabetic mice[J].Ann Plast Surg,2017,79(3):e15-e19.
[14] 丁越,张辉,孙晓菊.骨髓间充质干细胞促进皮肤创伤愈合及细胞因子在愈合过程表达研究[J].中国实用口腔科杂志,2015,8(2):103-107.
[15] Greaves NS,Ashcroft KJ,Baguneid M,et al.Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing [J].J Dermatol Sci,2013,72(3):206-217.
[16] 李晓娟,李硕,白冰珂,等.BALB/c突变卷毛小鼠皮肤组织结构的分析[J].中国比较医学杂志,2014,24(4):47-50.