川崎病患儿血清8-异构前列腺素的变化及其意义
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摘要
目的观察川崎病(KD)患儿血清8-异构前列腺素(8-ISO-PGF2a)水平变化,探讨其氧化应激在KD发病机制中的作用和与冠状动脉扩张的相关性。方法以30例健康儿童为对照组,将36例初发KD患儿根据心脏彩超结果分为冠状动脉病变组(CAL组)14例和无冠状动脉病变组(NCAL组)22例,采用酶联免疫法检测两组患儿血清8-ISO-PGF2a水平和肿瘤坏死因子TNF-α水平,运用超声心动图检测冠状动脉扩张程度,分析血清8-ISO-PGF2a水平变化及其与TNF-α水平和冠状动脉扩张程度的相关性。结果 CAL组和NCAL组患儿急性期和恢复期血清8-ISOPGF2a水平高于对照组,差异有统计学意义(P<0.05);CAL组急性期和恢复期血清8-ISO-PGF2a水平明显高于NCAL组,差异有统计学意义(P<0.05),且患儿血清8-ISO-PGF2a水平与TNF-α呈显著正相关(r=0.693,P<0.01);两组患儿血清8-ISO-PGF2a水平与冠状动脉扩张程度呈正相关(r=0.589,P<0.01)。结论 8-异构前列腺素可能参与KD患儿急性期血管炎的发病机制,且对评估KD冠状动脉损伤具有良好的临床价值。
Objective To observe the change of 8-ISO-PGF2aof patients with Kawasaki disease(KD)and discuss the role of its oxidative stress in pathogenesis of KD and relation with coronary artery dilation.Methods 30cases of healthy children were selected as control group,and 36cases of children patients with KD were divided into coronary artery lesion group(CAL group,with 14cases)and non coronary artery lesion group(non CAL group,with 22cases). Enzyme-linked immunoassay detection was used to detect the level of serum 8-ISO-PGF2aand tumor necrosis factor TNF-αof two groups with children patients,and echocardiography was used to detect the degree of coronary artery dilation and analyze relation between the change of serum 8-ISO-PGF2alevel and degree of coronary artery dilation.Results Serum 8-ISO-PGF2alevel of CAL group and NCAL group in acute phase and convalescence were higher than that of control group with statistically significant difference(P<0.05).Serum 8-ISO-PGF2alevel of CAL group in acute phase and convalescence was obviously higher than that of NCAL group with statistically significant difference(P<0.05)and serum 8-ISO-PGF2alevel of children patients was positively correlative with TNF-α(r=0.693,P<0.01).Serum 8-ISO-PGF2a level of children patients of two groups was positively correlative with coronary artery dilation(r=O.589,P<0.01). Conclusion 8-ISO-PGF2amay involve the pathogenesis of KD children with acute vasculitis and has good clinic value for the evaluation of KD coronary artery injury.
Objective To observe the change of 8-ISO-PGF2aof patients with Kawasaki disease(KD)and discuss the role of its oxidative stress in pathogenesis of KD and relation with coronary artery dilation.Methods 30cases of healthy children were selected as control group,and 36cases of children patients with KD were divided into coronary artery lesion group(CAL group,with 14cases)and non coronary artery lesion group(non CAL group,with 22cases). Enzyme-linked immunoassay detection was used to detect the level of serum 8-ISO-PGF2aand tumor necrosis factor TNF-αof two groups with children patients,and echocardiography was used to detect the degree of coronary artery dilation and analyze relation between the change of serum 8-ISO-PGF2alevel and degree of coronary artery dilation.Results Serum 8-ISO-PGF2alevel of CAL group and NCAL group in acute phase and convalescence were higher than that of control group with statistically significant difference(P<0.05).Serum 8-ISO-PGF2alevel of CAL group in acute phase and convalescence was obviously higher than that of NCAL group with statistically significant difference(P<0.05)and serum 8-ISO-PGF2alevel of children patients was positively correlative with TNF-α(r=0.693,P<0.01).Serum 8-ISO-PGF2a level of children patients of two groups was positively correlative with coronary artery dilation(r=O.589,P<0.01). Conclusion 8-ISO-PGF2amay involve the pathogenesis of KD children with acute vasculitis and has good clinic value for the evaluation of KD coronary artery injury.
引文
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[6]祁燕杰,李亚蕊.川崎病患儿血清巨噬细胞炎症蛋白-1含量变化及与冠状动脉扩张的相关性[J].临床儿科杂志,2013,31(6):519.
[7]张晗,谢利剑,黄敏,等.川崎病患儿外周血CD15抗原表达相关研究[J].临床儿科杂志,2013,31(5):433-435.
[8]廖先华,许玉霞,王建军.川崎病患儿血钾和血钠水平的变化及意义[J].西部医学,2010,22(9):1652-1654.
[2]张金旭.血清8-异构前列腺素变化与川崎病相关性的研究[D].吉林:吉林大学白求恩第一医院,2012:3.
[3]Schw edhelmE,TsikasD,DurandT,et al.Tandem mass spectrometric uantification of 8-iso-pristagland in F2nand itsmetabo lite2,3-dinor-5-dihydro,8-isoprostaglan in F2ain human urine[J].JChromatograph B,2000,744(1):99-112.
[4]杨波,李世钢.大剂量丙种球蛋白对川崎病患儿血管氧化应激的影响[J].中国动脉硬化杂志,2011,29(2):126-132.
[5]刘中生,费路.冠心病患者血清8-isoPGF2a水平检测及与冠脉病变Gensi ni积分的关系[J].山东医药,2011,51(3):50-51.
[6]祁燕杰,李亚蕊.川崎病患儿血清巨噬细胞炎症蛋白-1含量变化及与冠状动脉扩张的相关性[J].临床儿科杂志,2013,31(6):519.
[7]张晗,谢利剑,黄敏,等.川崎病患儿外周血CD15抗原表达相关研究[J].临床儿科杂志,2013,31(5):433-435.
[8]廖先华,许玉霞,王建军.川崎病患儿血钾和血钠水平的变化及意义[J].西部医学,2010,22(9):1652-1654.