CD1_D基因与B7-1基因真核表达载体的构建
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摘要
目的构建表达小鼠CD1_D基因和B7-1基因的真核表达载体。方法用PCR方法获得基因,定向克隆连接到真核表达载体上,得到重组真核表达载体,使用酶切方法加以鉴定。结果酶切得到的片段与所需相符,得到重组的逆转录病毒载体。结论重组小鼠CD1_D基因和B7-1基因真核表达载体的构建为今后进一步的研究奠定了基础。
Objective To construct recombinant eukaryotic vectors expressing B7-1 gene and CD1_D gene.Methods PCR was performed in order to get B7-1 gene and CD1_D gene,then they were ligated into eukaryotic vectors,and identified by digestion.Results The amplified fragments were coincident to the goal product.Recombinant eukaryotic vectors were obtained.Conclusions We got recombinant eukaryotic vectors expressing B7-1 gene and CD1_D gene which provides a foundation for the further study.
Objective To construct recombinant eukaryotic vectors expressing B7-1 gene and CD1_D gene.Methods PCR was performed in order to get B7-1 gene and CD1_D gene,then they were ligated into eukaryotic vectors,and identified by digestion.Results The amplified fragments were coincident to the goal product.Recombinant eukaryotic vectors were obtained.Conclusions We got recombinant eukaryotic vectors expressing B7-1 gene and CD1_D gene which provides a foundation for the further study.
引文
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[4]Spanoudakis E,Hu M,Naresh K,et al.Regulation of multiple myeloma survival and progression by CD1_D[J].Blood,2009,113(11):2498-2507.
[5]Ren SP,Wu CT,Huang WR,et al.Adenoviral-raediated transfer of human wild-type p53,GM-CSF and B7-1 genes results in growth suppression and autologous anti-tumor cytotoxicity of multiple myeloma cells in vitro[J].Cancer Immunol Immunother,2006,55(4):375-385.
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