流动注射-化学发光增强法测定L-半胱氨酸
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摘要
在碱性条件下,L-半胱氨酸对鲁米诺-过氧化氢反应所产生的化学发光有明显的增强作用.对L-半胱氨酸影响该反应体系化学发光强度的影响因素(鲁米诺、过氧化氢、pH值、泵速)进行实验研究,建立一种流动注射-化学发光增强法测定L-半胱氨酸的新方法.研究表明,在最佳实验条件下,该方法测定L-半胱氨酸的线性范围为2.0×10~(-6)～1.0×10~(-5) g/mL(r=0.997 5),检出限为3.5×10~(-7) g/mL,对6.0×10~(-6) g/mL的L-半胱氨酸进行11次平行测定,RSD为3.6%.
In alkaline medium, L-Cysteine has a significant enhancement effect on the chemiluminescence produced by the reaction of luminol and hydrogen peroxide. The influence factors of L-Cysteine on the chemiluminescence intensity of the reaction system(Luminol, hydrogen peroxide, pH, pump speed) were studied in detail. The analytical approach based on the system was established under the optimum experimental conditions. The CL intensity was linear with L-Cysteine concentration in the range of 2.0×10~(-6)～1.0×10~(-5) g/mL(r=0.997 5), and the detection limit was 3.5×10~(-7) g/mL. The relative standard deviation of 11 determinations was 3.6% for 6.0×10~(-6) g/mL L-Cysteine.
In alkaline medium, L-Cysteine has a significant enhancement effect on the chemiluminescence produced by the reaction of luminol and hydrogen peroxide. The influence factors of L-Cysteine on the chemiluminescence intensity of the reaction system(Luminol, hydrogen peroxide, pH, pump speed) were studied in detail. The analytical approach based on the system was established under the optimum experimental conditions. The CL intensity was linear with L-Cysteine concentration in the range of 2.0×10~(-6)～1.0×10~(-5) g/mL(r=0.997 5), and the detection limit was 3.5×10~(-7) g/mL. The relative standard deviation of 11 determinations was 3.6% for 6.0×10~(-6) g/mL L-Cysteine.
引文
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[3] 史伯安,郗娟,何治柯.流动注射-化学发光法测定半胱氨酸[J].分析测试学报,2005,24(6):102-105.
[4] 张苏,王凤.高效液相色谱法测定酶促反应液中的L-半胱氨酸含量[J].中国生化药物杂志,2009,30(5):318-320.
[5] CHEEVER K L,MARLOW K L,B’HYMER C,et al.Development of an HPLC-MS procedure for the quantification of N-acetyl-S-(n-propyl)-L-cysteine,the major urinary metabolite of 1-bromopropane in human urine[J].Journal of Chromatography B,2009,877(8/9):827-832.
[6] 陈述,白珊,王贞,等.牛血清白蛋白与纳米银的相互作用及半胱氨酸的检测[J].光谱实验室,2011,28(4):2047-2049.
[7] 曾飒,谭辉玲.抗坏血酸及L-Cys的流动注射动力学同时测定法[J].分析测试学报,2002,36(10):439-441.
[8] 周远华,辛妮,胡育筑.氨基酸分析新技术在药学研究中的应用[J].药学进展,2011,35(11):503-508.
[9] 孙汉文,连靠奇,梁淑轩,等.L-半胱氨酸在玻碳汞膜电极上的直接和间接测定[J].河北大学学报(自然科学版),2005,25(1):33-37.
[10] 方卢秋,李海芳,郑国金.流动注射化学发光法测定中药材中没食子酸[J].分析化学,2010,38(8):1110-1114.
[11] 赵丹华,訾言勤.流动注射化学发光增强法测定巯嘌呤的应用研究[J].分析试验室,2006,25(1):65-67.
[12] EDYTA N S,IWONA T,ANATOL K.Flow injection chemiluminescence determination of the total phenolics levels in plant-derived beverages using soluble manganese(Ⅳ)[J].Analytica Chimica Acta,2010,668(1):19-25.
[13] ZENG H J,YANG R,WANG Q W,et al.Determination of melamine by flow injection analysis based on chemiluminescence system[J].Food Chemistry,2011,127( 2):842-846.
[14] 李永新,赵丹华,卓淑娟,等.鲁米诺-四锰基磺酞菁-过氧化氢化学发光体系在蛋白质测定中的应用研究[J].分析化学,2003,31(5):638-640.
[15] ZHANG S C,WU Y Y,LI H.Chemiluminescence of cobalt(II)-hydrogen peroxide-hydrogencarbonate in the absence of luminescent reagents[J].Talanta,2000,53(3):609-612.