DNA对纳米CeO_2线类氧化酶活性的影响
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摘要
采用水热法合成纳米CeO_2线,使用透射电子显微镜、X射线衍射仪和拉曼光谱仪对其进行表征。使用傅里叶变换红外光谱仪和紫外可见分光光度计对DNA与纳米CeO_2线的相互作用进行分析,然后采用紫外可见分光光度法研究DNA对纳米CeO_2线类氧化酶活性的影响,并对体系进行pH影响以及特异性实验研究。结果表明,DNA抑制纳米CeO_2线的类氧化酶活性,这种抑制作用具有特异性。此工作将使纳米CeO_2得到进一步的实际应用。
The CeO_2 nanowires were synthesized by the hydrothermal method. The sample was characterized by transmission electron microscopy, X-ray diffractometer, and Raman spectrometer. Fourier transform infrared spectrometer and UV-Vis spectrophotometer were used to analyze the interaction between DNA and CeO_2 nanowires. The effect of DNA on the oxidase mimetic activity of CeO_2 nanowires was further studied by UV-Vis spectrophotometry. The impact of pH on the system and specificity experiment were also studied. The results show that DNA inhibits the oxidase activity of CeO_2 nanowires, and the inhibition is specific. This work further promotes the practical application of CeO_2 nanowires.
The CeO_2 nanowires were synthesized by the hydrothermal method. The sample was characterized by transmission electron microscopy, X-ray diffractometer, and Raman spectrometer. Fourier transform infrared spectrometer and UV-Vis spectrophotometer were used to analyze the interaction between DNA and CeO_2 nanowires. The effect of DNA on the oxidase mimetic activity of CeO_2 nanowires was further studied by UV-Vis spectrophotometry. The impact of pH on the system and specificity experiment were also studied. The results show that DNA inhibits the oxidase activity of CeO_2 nanowires, and the inhibition is specific. This work further promotes the practical application of CeO_2 nanowires.
引文
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[13] Pautler R, Kelly E Y, Huang P J J, et al. Attaching DNA to nanoceria: regulating oxidase activity and fluorescence quenching [J]. Acs Applied Materials & Interfaces, 2013, 5(15): 6 820-6 825.
[14] Zhang Y, Zhou K B, Zhai Y W, et al. Crystal plane effects of nano-CeO2 on its antioxidant activity [J]. Rsc Advances, 2014, 4(92): 50 325-50 330.
[15] Singh S, Dosani T, Karakoti A S, et al. A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties [J]. Biomaterials, 2011, 32(28): 6 745-6 753.
[2] Heckert E G, Seal S, Self W T. Fenton-like reaction catalyzed by the rare earth inner transition metal cerium [J]. Environmental Science & Technology, 2008, 42(13): 5 014-5 019.
[3] Heckert E G, Karakoti A S, Seal S, et al. The role of cerium redox state in the SOD mimetic activity of nanoceria [J]. Biomaterials, 2008, 29(18): 2 705-2 709.
[4] Asati A, Santra S, Kaittanis C, et al. Oxidase-like activity of polymer-coated cerium oxide nanoparticles [J]. Angewandte Chemie International Edition, 2009, 48(13): 2 308-2 312.
[5] Vernekar A A, Das T, Mugesh G. Vacancy-engineered nanoceria: enzyme mimetic hotspots for the degradation of nerve agents [J]. Angewandte Chemie International Edition, 2016, 55(4): 1 412-1 416.
[6] Liu B W, Huang Z C, Liu J W. Boosting the oxidase mimicking activity of nanoceria by fluoride capping: rivaling protein enzymes and ultrasensitive F- detection [J]. Nanoscale, 2016, 8(28): 13 562-13 567.
[7] Huang L J, Zhang W T, Chen K, et al. Facet-selective response of trigger molecule to CeO2 {110} for up-regulating oxidase-like activity [J]. Chemical Engineering Journal, 2017, 330: 746-752.
[8] Cheng H J, Lin S C, Muhammad F, et al. Rationally modulate the oxidase-like activity of nanoceria for self regulated bioassays [J]. Acs Sensors, 2016, 1(11): 1 336-1 343.
[9] Tana, Zhang M L, Li J, et al. Morphology-dependent redox and catalytic properties of CeO2 nanostructures: Nanowires, nanorods and nanoparticles [J]. Catalysis Today, 2009, 148(1): 179-183.
[10] Yang Y S, Mao Z, Huang W J, et al. Redox enzyme-mimicking activities of CeO2 nanostructures: Intrinsic influence of exposed facets [J]. Scientific Reports, 2016, 6: 35 344.
[11] Sisubalan N, Ramkumar V S, Pugazhendhi A, et al. ROS-mediated cytotoxic activity of ZnO and CeO2 nanoparticles synthesized using the Rubia cordifolia L. leaf extract on MG-63 human osteosarcoma cell lines [J]. Environmental Science and Pollution Research, 2017 (4): 1-11.
[12] Xu C, Liu Z, Wu L, et al. Nucleoside triphosphates as promoters to enhance nanoceria enzyme-like activity and for single-nucleotide polymorphism typing [J]. Advanced Functional Materials, 2014, 24 (11): 1 624-1 630.
[13] Pautler R, Kelly E Y, Huang P J J, et al. Attaching DNA to nanoceria: regulating oxidase activity and fluorescence quenching [J]. Acs Applied Materials & Interfaces, 2013, 5(15): 6 820-6 825.
[14] Zhang Y, Zhou K B, Zhai Y W, et al. Crystal plane effects of nano-CeO2 on its antioxidant activity [J]. Rsc Advances, 2014, 4(92): 50 325-50 330.
[15] Singh S, Dosani T, Karakoti A S, et al. A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties [J]. Biomaterials, 2011, 32(28): 6 745-6 753.