SFRP2对小鼠淋巴结总T细胞的作用研究
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摘要
目的:研究分泌型卷曲相关蛋白2(SFRP2)对C57小鼠淋巴结总T细胞功能的影响。方法:利用SFRP2重组蛋白构建高浓度SFRP2条件;体外分离培养C57小鼠淋巴结总T细胞;流式细胞术检测0.4 ng/mL SFRP2重组蛋白对C57小鼠淋巴结总T细胞的增殖、凋亡及亚群的影响。结果:0.4 ng/mL SFRP2重组蛋白抑制总T细胞的增殖和凋亡,主要抑制早期凋亡,对晚期凋亡率无明显影响;0.4 ng/mL SFRP2重组蛋白增加Th1亚群比例,降低Th2亚群比例,提高了Th1/Th2比例。结论:一定浓度SFRP2重组蛋白可以抑制总T细胞的增殖和凋亡,并促进T细胞向Th1亚群分化,抑制其向Th2亚群分化。
Objective: To study the effect of secreted frizzled-related protein 2(SFRP2) on lymph node T cells in C57 mice. Methods: SFRP2 recombinant protein was used to construct high concentration SFRP2. The total T cells of lymph node from C57 mouse were isolated and cultured in vitro. The effects of SFRP2 recombinant protein on proliferation, apoptosis and subpopulation of lymph node T cells in C57 mice were detected by flow cytometry. Results: SFRP2(0.4 ng/mL) inhibited the proliferation of total T cells;SFRP2(0.4 ng/mL)inhibited the early apoptosis of total T cells, has no significant effect on late apoptosis rate, and inhibits total apoptosis rate; SFRP2(0.4 ng/mL) increases the proportion of Th1 subpopulations, decreases the proportion of Th2 subpopulations, and changes the Th1/Th2 balance. Conclusions: The results indicate that the concentration of SFRP2(0.4 ng/mL) can inhibit the proliferation of total T cells, promote apoptosis, and affect the balance of Th1/Th2.
Objective: To study the effect of secreted frizzled-related protein 2(SFRP2) on lymph node T cells in C57 mice. Methods: SFRP2 recombinant protein was used to construct high concentration SFRP2. The total T cells of lymph node from C57 mouse were isolated and cultured in vitro. The effects of SFRP2 recombinant protein on proliferation, apoptosis and subpopulation of lymph node T cells in C57 mice were detected by flow cytometry. Results: SFRP2(0.4 ng/mL) inhibited the proliferation of total T cells;SFRP2(0.4 ng/mL)inhibited the early apoptosis of total T cells, has no significant effect on late apoptosis rate, and inhibits total apoptosis rate; SFRP2(0.4 ng/mL) increases the proportion of Th1 subpopulations, decreases the proportion of Th2 subpopulations, and changes the Th1/Th2 balance. Conclusions: The results indicate that the concentration of SFRP2(0.4 ng/mL) can inhibit the proliferation of total T cells, promote apoptosis, and affect the balance of Th1/Th2.
引文
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[7] Rattis FM,Voermans C,Reya T.Wnt signaling in the stem cell niche[J].Curr Opin Hematol,2004,11(2):88-94.
[8] Yu G,Wang J,Lin X,et al.Demethylation of SFRP2 by histone demethylase KDM2A regulated osteo-dentinogenic differentiation of stem cells of the apical papilla[J].Cell Prolif,2016,49(3):330-340.
[9] Liu D,Wang Y,Jia Z,et al.Demethylation of IGFBP5 by Histone Demethylase KDM6B Promotes Mesenchymal Stem Cell-Mediated Periodontal Tissue Regeneration by Enhancing Osteogenic Differentiation and Anti-Inflammation Potentials[J].Stem Cells,2015,33(8):2523-2536.
[10] Le Blanc K,Mougiakakos D.Multipotent mesenchymal stromal cells and the innate immune system[J].Nat Rev Immunol,2012,12(5):383-396.
[11] Bernardo ME,Fibbe WE.Mesenchymal stromal cells:sensors and switchers of inflammation[J].Cell Stem Cell,2013,13(4):392-402.
[12] Liu Y,Wang S,Shi S.The role of recipient T cells in mesenchymal stem cell-based tissue regeneration[J].Int J Biochem Cell Biol,2012,44(11):2044-2050.
[13] Mescher AL,Neff AW,King MW.Inflammation and immunity in organ regeneration[J].Dev Comp Immunol,2017,66:98-110.
[14] Brant JO,Yoon JH,Polvadore T,et al.Cellular events during scar-free skin regeneration in the spiny mouse,Acomys[J].Wound Repair Regen,2016,24(1):75-88.
[15] Mescher AL,Neff AW,King MW.Changes in the Inflammatory Response to Injury and Its Resolution during the Loss of Regenerative Capacity in Developing Xenopus Limbs[J].PLoS One,2013,8(11):e80477.
[16] Tidball JG,Villalta SA.Regulatory interactions between muscle and the immune system during muscle regeneration[J].Am J Physiol Regul Integr Comp Physiol,2010,298(5):R1173-R118.
[17] Ramasamy R,Lam EW,Soeiro I,et al.Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells:impact on in vivo tumor growth[J].Leukemia,2006,21(2):304-310.
[18] Lim JY,Park MJ,Im KI,et al.Combination cell therapy using mesenchymal stem cells and regulatory T-cells provides a synergistic immunomodulatory effect associated with reciprocal regulation of TH1/TH2 and th17/treg cells in a murine acute graft-versus-host disease model[J].Cell Transplant,2014,23(6):703-714.
[19] Yamaza T,Kentaro A,Chen C,et al.Immunomodulatory proper-ties of stem cells from human exfoliated deciduous teeth[J].Stem Cell Res Ther,2010,1(1):5.