替吉奥下调大鼠胃癌前病变组织Notch1和Jagged1的表达
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摘要
目的:探讨替吉奥治疗大鼠胃癌前病变的效果以及对Notch信号通路蛋白Notch1和Jagged1表达情况的影响。方法:将60只大鼠随机取等分3组:对照组、模型组、替吉奥治疗组。用100μg/ml的MNNG溶液灌胃,制作大鼠胃癌模型。模型建立成功后治疗组给予替吉奥治疗药物,对照组以及模型组给予等体积的生理盐水。PCR法检测胃癌前病期组织Notch信号通路Notch1和Jagged1的基因表达水平; Western blot检测Notch1和Jagged的蛋白表达量。结果:PCR检测结果显示,Notch1和Jagged1在对照组中仅有少量的表达,与对照组相比,模型组的表达出现了显著增高(P<0. 05),相比与模型组,替吉奥治疗组表达有明显下降,仍高于对照组的表达(P<0. 05); Western blot检测结果表明,Notch1和Jagged1在对照组中蛋白仅有微量的基础表达,与对照组相比,模型组的两蛋白表达水平明显地上升(P<0. 05),同模型组相比,替吉奥治疗组的两蛋白表达均有所下降,但仍高于对照组(P<0. 05)。结论:替吉奥治疗可显著降低大鼠胃癌前病变组织Notch1和Jagged1蛋白的表达。
Objective: To explore the effect of S-1( Tegafur,Gimeracil and Oteracil Porassium Capsules) treatment on the expression of Notch signaling pathway protein Notch1 and Jagged1 in rat gastric precancerous lesions. Methods: 60 healthy male SD rats were randomly and equally divided into three groups: control group,model group,and S-1 treatment group. The rat gastric cancer model was established by the intragastric administration of 100 μg/ml MNNG solution. Rats with gastric precancerous lesions were treated with S-1 or saline. The mRNA levels of Notch1 and Jagged1 were detected by PCR. The protein levels of Notch1 and Jagged1 were detected by Western blot. Results: PCR showed that Notch1 and Jagged1 mRNA levels were significantly higher in the gastric tissues of model group than that of control group; S-1 treatment could partially decrease the mRNA levels of these two genes( P<0. 05). Consistent with the PCR result,Western blot result showed that S-1 treatment decreased the levels of Notch1 and Jagged1 protein in rat gastric precancerous lesions. Conclusion: S-1 treatment can decrease Notch1 and Jagged1 expression in rat gastric precancerous lesions.
Objective: To explore the effect of S-1( Tegafur,Gimeracil and Oteracil Porassium Capsules) treatment on the expression of Notch signaling pathway protein Notch1 and Jagged1 in rat gastric precancerous lesions. Methods: 60 healthy male SD rats were randomly and equally divided into three groups: control group,model group,and S-1 treatment group. The rat gastric cancer model was established by the intragastric administration of 100 μg/ml MNNG solution. Rats with gastric precancerous lesions were treated with S-1 or saline. The mRNA levels of Notch1 and Jagged1 were detected by PCR. The protein levels of Notch1 and Jagged1 were detected by Western blot. Results: PCR showed that Notch1 and Jagged1 mRNA levels were significantly higher in the gastric tissues of model group than that of control group; S-1 treatment could partially decrease the mRNA levels of these two genes( P<0. 05). Consistent with the PCR result,Western blot result showed that S-1 treatment decreased the levels of Notch1 and Jagged1 protein in rat gastric precancerous lesions. Conclusion: S-1 treatment can decrease Notch1 and Jagged1 expression in rat gastric precancerous lesions.
引文
[1]孙秀娣,牧人,周有尚,等.中国胃癌死亡率20年变化情况分析及其发展趋势预测[J].中华肿瘤杂志,2004,26(1):4-9.Sun XD,Mu R,Zhou YS,et al. Analysis of the 20-year changes in gastric cancer mortality in China and its development trend prediction[J].Chin J Oncol,2004,26(1):4-9.
[2] Van Cutsem E,de Haas S,Kang YK,et al.Bevacizumab in combination with chemotherapy as first-line therapy in advanced gastric cancer:a biomarker evaluation from the AVAGAST randomized phaseⅢtrial[J].J Clin Oncol,2012,30(17):2119-2127.
[3]吴瑕,何君,王文军. miR-107靶向NID2通过Notch信号通路调控肺癌细胞的增殖侵袭[J].中国免疫学杂志,2017,33(11):1662-1667.Wu X,He J,Wang WJ. Mir-107 targeted NID2 to regulate proliferation of lung cancer cells through Notch signaling pathway[J]. J Chin Immunol,2017,33(11):1662-1667.
[4] Sun HW,Wu C,Tan HY,et al.Combination DLL4 with Jagged1-siRNA can enhance inhibition of the proliferation and invasiveness activity of human gastric carcinoma by Notch1/VEGF pathway[J].Hepatogastroenterol,2012,59(115):924-929.
[5]王春斐,李登欣,桂忠娜.胃癌组织中Notch1、Jagged1表达变化及意义[J].山东医药,2016,56(10):40-41.Wang CF,Li DX,Gui ZN. Expression and significance of Notch1and Jagged1 in gastric cancer tissues[J].Shandong Med,2016,56(10):40-41.
[6]李纪远,张灿斌,马新,等.长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响[J].中国免疫学杂志,2017,33(9):1392-1397.Li JY,Zhang CB,Ma X,et al.Effects of long chain non-coding H19targeted regulation on the invasion and migration of lung cancer through the Notch pathway[J]. J Chin Immunol,2017,33(9):1392-1397.
[7]丁海霞,赵连梅,单保恩.肿瘤相关巨噬细胞和CCL5在胃癌中的表达及其相关性研究[J].中国免疫学杂志,2016,32(1):74-78.Ding HX,Zhao LM,Shan BE. The expression and correlation of tumor related macrophages and CCL5 in gastric cancer[J]. J Chin Immunol,2016,32(1):74-78.
[8]陆建邦.胃癌发病因素的流行病学研究进展[J].肿瘤防治研究,2001,28(2):157-159.Lu JB. Advances in epidemiological studies on the pathogenesis of gastric cancer[J]. Cancer Prevention Control,2001,28(2):157-159.
[9]周春颜,刘丽波,谭爱萍.替吉奥的作用机制及临床应用的研究发展[J].中外医疗,2012,31(13):109-110.Zhou CY,Liu LB,Tan AP. Research and development of the mechanism and clinical application of diageo[J]. Chin Foreign Med,2012,31(13):109-110.
[10]陈颖,杨大明,邵俊,等.替吉奥单药新辅助化疗对进展期胃癌的影响[J][J].中国医药导刊,2012,14(1):43-44.Chen Y,Yang DM,Shao J,et al.Effect of neoadjuvant chemotherapy on advanced gastric cancer[J]. Chin J Med,2012,14(1):43-44.
[2] Van Cutsem E,de Haas S,Kang YK,et al.Bevacizumab in combination with chemotherapy as first-line therapy in advanced gastric cancer:a biomarker evaluation from the AVAGAST randomized phaseⅢtrial[J].J Clin Oncol,2012,30(17):2119-2127.
[3]吴瑕,何君,王文军. miR-107靶向NID2通过Notch信号通路调控肺癌细胞的增殖侵袭[J].中国免疫学杂志,2017,33(11):1662-1667.Wu X,He J,Wang WJ. Mir-107 targeted NID2 to regulate proliferation of lung cancer cells through Notch signaling pathway[J]. J Chin Immunol,2017,33(11):1662-1667.
[4] Sun HW,Wu C,Tan HY,et al.Combination DLL4 with Jagged1-siRNA can enhance inhibition of the proliferation and invasiveness activity of human gastric carcinoma by Notch1/VEGF pathway[J].Hepatogastroenterol,2012,59(115):924-929.
[5]王春斐,李登欣,桂忠娜.胃癌组织中Notch1、Jagged1表达变化及意义[J].山东医药,2016,56(10):40-41.Wang CF,Li DX,Gui ZN. Expression and significance of Notch1and Jagged1 in gastric cancer tissues[J].Shandong Med,2016,56(10):40-41.
[6]李纪远,张灿斌,马新,等.长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响[J].中国免疫学杂志,2017,33(9):1392-1397.Li JY,Zhang CB,Ma X,et al.Effects of long chain non-coding H19targeted regulation on the invasion and migration of lung cancer through the Notch pathway[J]. J Chin Immunol,2017,33(9):1392-1397.
[7]丁海霞,赵连梅,单保恩.肿瘤相关巨噬细胞和CCL5在胃癌中的表达及其相关性研究[J].中国免疫学杂志,2016,32(1):74-78.Ding HX,Zhao LM,Shan BE. The expression and correlation of tumor related macrophages and CCL5 in gastric cancer[J]. J Chin Immunol,2016,32(1):74-78.
[8]陆建邦.胃癌发病因素的流行病学研究进展[J].肿瘤防治研究,2001,28(2):157-159.Lu JB. Advances in epidemiological studies on the pathogenesis of gastric cancer[J]. Cancer Prevention Control,2001,28(2):157-159.
[9]周春颜,刘丽波,谭爱萍.替吉奥的作用机制及临床应用的研究发展[J].中外医疗,2012,31(13):109-110.Zhou CY,Liu LB,Tan AP. Research and development of the mechanism and clinical application of diageo[J]. Chin Foreign Med,2012,31(13):109-110.
[10]陈颖,杨大明,邵俊,等.替吉奥单药新辅助化疗对进展期胃癌的影响[J][J].中国医药导刊,2012,14(1):43-44.Chen Y,Yang DM,Shao J,et al.Effect of neoadjuvant chemotherapy on advanced gastric cancer[J]. Chin J Med,2012,14(1):43-44.