康复新液对溃疡性结肠炎大鼠肠道菌群的影响
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摘要
目的:研究康复新液对2,4-二硝基氯苯(DNCB)联合醋酸诱导溃疡性结肠炎(UC)大鼠肠道菌群的影响。方法:采用DNCB联合醋酸建立UC模型,造模后各组分别灌肠给予美沙拉嗪,康复新液低、中、高剂量药物,正常对照组与模型对照组给予0.9%氯化钠溶液。末次给药后处死大鼠,收集粪便样本,采用荧光定量PCR法检测各组大鼠粪便样本中主要菌种数量的变化。结果:经药物治疗后,与模型对照组比较,康复新液各剂量组拟杆菌属、双歧杆菌属增加,康复新液低剂量组乳杆菌属增加;康复新液低剂量组大肠埃希菌及康复新液中、高剂量组肠球菌属显著减少(P<0.05)。结论:康复新液可通过调节肠道菌群,使益生菌数量增加,条件致病菌数量减少,平衡肠道菌群辅助UC的治疗。
Objective: To study the effect of Kangfuxin liquid on gut flora in rats with ulcerative colitis induced by 2,4-dinitrochlorobenzene combined with acetic acid. Methods: Rat models of UC were established by using DNCB combined with acetic acid. Groups of UC were treated with Mesalazine, and low, medium and high doses of Kangfuxin respectively. Normal saline was given to the control group and the model group. After their final treatment rats were sacrificed, and fecal samples were collected.Fluorescence quantitative PCR was used to detect the changes in the number of major bacterial species in fecal samples of rats in each group. Results: Compared with the model group, the number of Bacteroides and Bifidobacterium increased in all the groups treated with Kangfuxin. The number of Lactobacillus increased and the number of Escherichia coli significantly reduced in low-dose Kangfuxin group; Enterococcus significantly decreased in mediumdose and highliquid can improve people's gut bacteria and help control ulcerative colitis by increasing the number of probiotics, reducing the number of opportunistic pathogens and balancing gut flora.
Objective: To study the effect of Kangfuxin liquid on gut flora in rats with ulcerative colitis induced by 2,4-dinitrochlorobenzene combined with acetic acid. Methods: Rat models of UC were established by using DNCB combined with acetic acid. Groups of UC were treated with Mesalazine, and low, medium and high doses of Kangfuxin respectively. Normal saline was given to the control group and the model group. After their final treatment rats were sacrificed, and fecal samples were collected.Fluorescence quantitative PCR was used to detect the changes in the number of major bacterial species in fecal samples of rats in each group. Results: Compared with the model group, the number of Bacteroides and Bifidobacterium increased in all the groups treated with Kangfuxin. The number of Lactobacillus increased and the number of Escherichia coli significantly reduced in low-dose Kangfuxin group; Enterococcus significantly decreased in mediumdose and highliquid can improve people's gut bacteria and help control ulcerative colitis by increasing the number of probiotics, reducing the number of opportunistic pathogens and balancing gut flora.
引文
[1]孙中美,胡立明,毛堂友,等.溃疡性结肠炎中西医治疗进展[J].辽宁中医药大学学报,2018,20(11):171-175.
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[9]宋美茹,姚萍.应用实时荧光定量PCR定量检测溃疡性结肠炎肠道大肠埃希菌乳酸杆菌及双歧杆菌属的变化[J].中国微生态学杂志,2012,24(3):239-243.
[10]刘伟,刘翔,林漫鹏,等.溃疡性结肠炎患者正常与病变肠段肠道菌群比较[J].湖北民族学院学报(医学版),2011,28(1):4-6.
[11]孙勇,丁彦青.溃疡性结肠炎患者肠道菌群与病理变化关系的探讨[J].现代消化及介入诊疗,2009,14(1):26-28.
[12]ANDOH A,IMAEDA H,AOMATSU T,et al.Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn’s disease using terminal restriction fragment length polymorphism analysis[J].J Gastroenterol,2011,46(4):479-486.
[13]徐晓玲,柴海娜,孙超,等.扬州市初诊为溃疡性结肠炎患者的肠道菌群变化及意义[J].国际消化病杂志,2017,37(2):117-119.
[14]张瑶,颜宏利,周海玥,等.化学法建立溃疡性结肠炎动物模型的研究进展[J].世界华人消化杂志,2015,23(27):4384-4392.
[15]TREMAROLI V,BACKHED F.Functional interactions between the gutmicrobiota and host metabolism[J].Nature,2012,489(7415):242-249.
[16]李旻.人体肠道菌群结构与宿主代谢的相关性研究[D].上海:上海交通大学,2009.
[2]臧思源,柳越冬.溃疡性结肠炎与粪便菌群移植研究进展[J].辽宁中医药大学学报,2018,20(11):101-104.
[3]FEDORAK R N,MADSEN K L.Probiotics and the management of inflammatory bowel disease[J].Inflammatory Bowel Diseases,2004,10(3):286-299.
[4]罗廷顺,高孟婷,马芳芳,等.美洲大蠊药理作用及临床应用研究进展[J].安徽农业科学,2012,40(10):5933-5942.
[5]王朋川,何苗,张汉超,等.康复新液对2,4-二硝基氯苯联合醋酸诱导大鼠溃疡性结肠炎的影响[J].大理大学学报,2016,1(4):17-21.
[6]HAMAMOTO N,MAEMURA K,HIRATA I,et al.Inhibition of dextran sulphate sodium(DSS)-induced colitis in mice by intracilonically administered antibodies against adhesion molecules(endothelial leucoyte adhesion molecule-1(ELAM-1)or intercellular adhesion molecule-1(ICAM-1)[J].Clin Exp Immunol,1999,117:462-468.
[7]程悦,左浩江,廖虹瑜,等.肠道常见菌群荧光定量PCR检测方法的建立[J].现代预防医学,2014,41(23):4338-4341.
[8]蒋曼,高鸿亮,姚萍.RT-PCR技术定量检测新疆溃疡性结肠炎患者肠道菌群的改变[J].世界华人消化杂志,2014,22(4):596-600.
[9]宋美茹,姚萍.应用实时荧光定量PCR定量检测溃疡性结肠炎肠道大肠埃希菌乳酸杆菌及双歧杆菌属的变化[J].中国微生态学杂志,2012,24(3):239-243.
[10]刘伟,刘翔,林漫鹏,等.溃疡性结肠炎患者正常与病变肠段肠道菌群比较[J].湖北民族学院学报(医学版),2011,28(1):4-6.
[11]孙勇,丁彦青.溃疡性结肠炎患者肠道菌群与病理变化关系的探讨[J].现代消化及介入诊疗,2009,14(1):26-28.
[12]ANDOH A,IMAEDA H,AOMATSU T,et al.Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn’s disease using terminal restriction fragment length polymorphism analysis[J].J Gastroenterol,2011,46(4):479-486.
[13]徐晓玲,柴海娜,孙超,等.扬州市初诊为溃疡性结肠炎患者的肠道菌群变化及意义[J].国际消化病杂志,2017,37(2):117-119.
[14]张瑶,颜宏利,周海玥,等.化学法建立溃疡性结肠炎动物模型的研究进展[J].世界华人消化杂志,2015,23(27):4384-4392.
[15]TREMAROLI V,BACKHED F.Functional interactions between the gutmicrobiota and host metabolism[J].Nature,2012,489(7415):242-249.
[16]李旻.人体肠道菌群结构与宿主代谢的相关性研究[D].上海:上海交通大学,2009.