‘冬枣’的五个自然变异材料评价
|
摘要
以‘冬枣’及其5个自然变异材料为试材,对其果实形态、果实营养、物候期、染色体倍性以及DNA指纹进行了比较分析,以期筛选出综合性状优异的‘冬枣’变异类型。结果表明:与普通‘冬枣’相比,变异1号材料为大果型,其单果质量显著增加,为25.22g,果实可溶性糖含量和糖酸比提高。在物候期上,变异1号和2号材料与‘冬枣’表现相近,但其它3个变异材料的果实成熟期比‘冬枣’晚约15d。流式细胞仪分析显示5个自然变异材料均为二倍体,15对SSR引物检测表明5个自然变异材料与‘冬枣’基因组DNA的SSR指纹相同,可能均为‘冬枣’的自然芽变。评价结果显示,变异1号属于优良大果变异,变异3、4、5号为晚熟变异。
Ziziphus jujuba Mill.‘Dongzao'and its five natural variants were taken as materials,comparisons of fruit morphology,fruit nutrition,phonological period,chromosome ploidy and DNA fingerprint were carried out,in order to selecte the variation type of ‘Dongzao'with excellent comprehensive charaters.The results showed that compared with ‘Dongzao',the variant No.1 had significantly bigger fruits with the single fruit weight of 25.22 g and higher soluble sugar content and acid-sugar ratio.The phonological period of variation No.1 and No.2 was similar to that of‘Dongzao',yet other three variants fruits matured 15 days later than ‘Dongzao'.All the five natural variants were diploidy according to flow cytometry analysis.No difference of DNA fingerprint among‘Dongzao'and five natural variants were observed using 15 pairs of SSR primers,which indicated that the five variants were the most probably bud mutations from ‘Dongzao'.The evaluation demonstrated that the variant No.1was a big fruit type and the variant No.3,No.4 and No.5 belonged to late-maturing fruit type.
Ziziphus jujuba Mill.‘Dongzao'and its five natural variants were taken as materials,comparisons of fruit morphology,fruit nutrition,phonological period,chromosome ploidy and DNA fingerprint were carried out,in order to selecte the variation type of ‘Dongzao'with excellent comprehensive charaters.The results showed that compared with ‘Dongzao',the variant No.1 had significantly bigger fruits with the single fruit weight of 25.22 g and higher soluble sugar content and acid-sugar ratio.The phonological period of variation No.1 and No.2 was similar to that of‘Dongzao',yet other three variants fruits matured 15 days later than ‘Dongzao'.All the five natural variants were diploidy according to flow cytometry analysis.No difference of DNA fingerprint among‘Dongzao'and five natural variants were observed using 15 pairs of SSR primers,which indicated that the five variants were the most probably bud mutations from ‘Dongzao'.The evaluation demonstrated that the variant No.1was a big fruit type and the variant No.3,No.4 and No.5 belonged to late-maturing fruit type.
引文
[1]刘孟军,汪民.中国枣种质资源[M].北京:中国林业出版社,2009:56-57.
[2]刘孟军,王玖瑞,刘平,等.中国枣生产与科研成就及前沿进展[J].园艺学报,2015,42(9):1683-1698.
[3]刘孟军,王玖瑞,刘平,等.枣树免去雄杂交育种的设计与实践[J].园艺学报,2014,41(7):1495-1502.
[4]李志欣,刘进余,张立树,等.优质金丝小枣新品种沧金1号的选育[J].河北农业科学,2011,15(1):68-69.
[5]王振亮,邵学红,张东风,等.抗缩果病和抗裂果枣树新品种‘曙光3号’[J].园艺学报,2012,39(4):803-084.
[6]单公华,周广芳,张琼,等.枣制干鲜食兼用新品种鲁枣8号的选育[J].中国果树,2013(5):8-10,85.
[7]单公华,周广芳,张琼,等.晚熟鲜食枣新品种‘鲁枣6号’[J].园艺学报,2012,39(7):1409-1410.
[8]单公华,周广芳,张琼,等.枣晚熟鲜食新品种鲁枣11号的选育[J].中国果树,2014(2):5-8.
[9]王森,谢碧霞,钟秋平,等.枣新品种‘中秋酥脆枣’[J].园艺学报,2009,36(5):771,781.
[10]王振亮,肖家良,韩会智,等.短枝型芽变枣新品种‘沧冬1号’[J].园艺学报,2012,39(6):1213-1214.
[11]王翠香,赵登超,孙超,等.特晚熟枣新品种‘傲雪’的选育[J].果树学报,2016,33(8):1030-1032.
[12]刘颖,冯春芳,于洪长,等.‘冬枣’天然二、四混倍体的发现与评价[J].园艺学报,2016,43(5):966-974.
[13]马庆华,李永红,梁丽松,等.冬枣优良单株综合评价体系的建立[J].农业系统科学与综合研究,2011,27(3):321-327.
[14]肖京.枣基因组SSR位点特征分析及引物开发[D].保定:河北农业大学,2014.
[15]XIAO J,ZHAO J,LIU M J,et al.Genome-wide characterization of simple sequence repeat(SSR)loci in Chinese jujube and jujube SSR primer transferability[J].PLoS One,2015,10(5):e0127812.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[17]陈莹莹.枣和酸枣果实抗坏血酸代谢机制研究[D].保定:河北农业大学,2015.
[2]刘孟军,王玖瑞,刘平,等.中国枣生产与科研成就及前沿进展[J].园艺学报,2015,42(9):1683-1698.
[3]刘孟军,王玖瑞,刘平,等.枣树免去雄杂交育种的设计与实践[J].园艺学报,2014,41(7):1495-1502.
[4]李志欣,刘进余,张立树,等.优质金丝小枣新品种沧金1号的选育[J].河北农业科学,2011,15(1):68-69.
[5]王振亮,邵学红,张东风,等.抗缩果病和抗裂果枣树新品种‘曙光3号’[J].园艺学报,2012,39(4):803-084.
[6]单公华,周广芳,张琼,等.枣制干鲜食兼用新品种鲁枣8号的选育[J].中国果树,2013(5):8-10,85.
[7]单公华,周广芳,张琼,等.晚熟鲜食枣新品种‘鲁枣6号’[J].园艺学报,2012,39(7):1409-1410.
[8]单公华,周广芳,张琼,等.枣晚熟鲜食新品种鲁枣11号的选育[J].中国果树,2014(2):5-8.
[9]王森,谢碧霞,钟秋平,等.枣新品种‘中秋酥脆枣’[J].园艺学报,2009,36(5):771,781.
[10]王振亮,肖家良,韩会智,等.短枝型芽变枣新品种‘沧冬1号’[J].园艺学报,2012,39(6):1213-1214.
[11]王翠香,赵登超,孙超,等.特晚熟枣新品种‘傲雪’的选育[J].果树学报,2016,33(8):1030-1032.
[12]刘颖,冯春芳,于洪长,等.‘冬枣’天然二、四混倍体的发现与评价[J].园艺学报,2016,43(5):966-974.
[13]马庆华,李永红,梁丽松,等.冬枣优良单株综合评价体系的建立[J].农业系统科学与综合研究,2011,27(3):321-327.
[14]肖京.枣基因组SSR位点特征分析及引物开发[D].保定:河北农业大学,2014.
[15]XIAO J,ZHAO J,LIU M J,et al.Genome-wide characterization of simple sequence repeat(SSR)loci in Chinese jujube and jujube SSR primer transferability[J].PLoS One,2015,10(5):e0127812.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[17]陈莹莹.枣和酸枣果实抗坏血酸代谢机制研究[D].保定:河北农业大学,2015.