甜糯双隐性基因型玉米种质的创制与评价
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摘要
【目的】创制甜糯基因双隐性玉米自交系,了解甜糯基因双隐性对品质性状的影响,为甜糯玉米育种提供参考和依据。【方法】以1份甜玉米Zea mays ssp. saccharata骨干自交系(M01)和3份糯玉米Z. mays var. ceratina自交系(L33、L35和L38)为材料,用与糯玉米隐性基因wx紧密连锁的分子标记辅助筛选,F2代得到甜糯双隐性玉米材料,连续自交,F4代得到11个纯合甜糯双隐性玉米自交系,检测其可溶性糖含量、淀粉含量及乳熟期籽粒果皮厚度,并与对应的甜、糯玉米亲本进行比较分析。【结果】与wx基因紧密连锁的引物phi061在甜、糯玉米亲本间扩增出清晰且呈共显性的条带,可在F2代作为糯质基因的前景选择标记。11份甜糯双隐性自交系材料的可溶性糖质量分数平均为12.27%,比甜玉米亲本高2.55%,远高于糯玉米亲本;淀粉质量分数平均为19.73%,比甜玉米亲本低3.77%,远低于糯玉米亲本;乳熟期籽粒冠部和背胚部果皮厚度均介于对应的杂交亲本之间。【结论】通过分子标记辅助选择可快速创制甜糯纯合双隐性玉米材料,其可溶性糖含量高于甜质亲本,淀粉含量低于甜质亲本,果皮厚度介于双亲之间。
【Objective】To create double recessive sweet-waxy corn inbred lines, identify the effects of double recessive sweet-waxy genes on quality traits, and provide a reference and basis for sweet-waxy corn breeding.【Method】The double recessive sweet-waxy corn materials with wxwxsh2 sh2 genotype were screened by molecular marker that linked with waxy corn recessive gene wx in F2 populations obtained from one elite sweet corn line(M01) and three waxy corn lines(L33, L35 and L38). Then these materials were self-pollinated continuously, and 11 pure double recessive sweet-waxy corn inbred lines with wxwxsh2 sh2 genotype were obtained in F4 populations. Their soluble sugar content, total starch content and pericarp thickness were measured and compared with the corresponding sweet corn parent and waxy corn parent. 【Result】The primer phi061, which was tightly linked to wx gene, amplified clear and codominant bands between sweet corn parent and waxy corn parent and was selected as the foreground selection marker of waxy gene. The average content of soluble sugar was 12.27% for double recessive sweet-waxy corn inbred lines, which was 2.55% higher than that sweet corn parent and far higher than that waxy corn parent. The average total starch content was 19.73%, which was 3.77% lower than that sweet corn parent, and far lower than that waxy corn parent. The pericarp thicknesses of crown and abgerminal regions of double recessive maize lines were in the interval of their corresponding parents. 【Conclusion】It is a feasible way to create rapidly the double recessive sweet-waxy corn lines by molecular marker assisted selection. Their average content of soluble sugar is higher than that of sweet corn parent, the average content of starch is lower than that of sweet parent, and the average pericarp thickness is between their parents.
【Objective】To create double recessive sweet-waxy corn inbred lines, identify the effects of double recessive sweet-waxy genes on quality traits, and provide a reference and basis for sweet-waxy corn breeding.【Method】The double recessive sweet-waxy corn materials with wxwxsh2 sh2 genotype were screened by molecular marker that linked with waxy corn recessive gene wx in F2 populations obtained from one elite sweet corn line(M01) and three waxy corn lines(L33, L35 and L38). Then these materials were self-pollinated continuously, and 11 pure double recessive sweet-waxy corn inbred lines with wxwxsh2 sh2 genotype were obtained in F4 populations. Their soluble sugar content, total starch content and pericarp thickness were measured and compared with the corresponding sweet corn parent and waxy corn parent. 【Result】The primer phi061, which was tightly linked to wx gene, amplified clear and codominant bands between sweet corn parent and waxy corn parent and was selected as the foreground selection marker of waxy gene. The average content of soluble sugar was 12.27% for double recessive sweet-waxy corn inbred lines, which was 2.55% higher than that sweet corn parent and far higher than that waxy corn parent. The average total starch content was 19.73%, which was 3.77% lower than that sweet corn parent, and far lower than that waxy corn parent. The pericarp thicknesses of crown and abgerminal regions of double recessive maize lines were in the interval of their corresponding parents. 【Conclusion】It is a feasible way to create rapidly the double recessive sweet-waxy corn lines by molecular marker assisted selection. Their average content of soluble sugar is higher than that of sweet corn parent, the average content of starch is lower than that of sweet parent, and the average pericarp thickness is between their parents.
引文
[1]李小琴,王青峰.广东省甜玉米发展现状与对策探讨[J].作物杂志,2007(3):32-34.
[2]刘蔚楠,万忠,甘阳英,等.2015年广东甜玉米产业发展形势与对策建议[J].广东农业科学,2016,43(3):12-16.
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[7]张沛敏,邵林生,闫建宾,等.黑甜糯玉米新品种黑甜糯631的选育[J].中国蔬菜,2018(5):74-76.
[8]王萍,田凤芝,李晨.北方甜糯玉米优质高效栽培及发展前景[J].吉林蔬菜,2016(6):18-19.
[9]郝德荣,冒宇翔,陈国清,等.我国鲜食甜糯玉米育种现状与展望[J].浙江农业科学,2016,57(4):478-481.
[10]CREECH R G,MCARDLE F J.Gene interaction for quantitative changes in carbohydrates in maize kernels 1[J].Crop Sci,1966,6(2):192-194.
[11]李新海,白丽,彭泽斌,等.糯玉米育种技术研究进展[J].玉米科学,2003(专刊):14-16.
[12]杨耀迥,张述宽,滕辉升,等.应用分子标记辅助选择甜糯双隐基因型玉米种质[J].广西农业科学,2010,41(1):1-3.
[13]DOYLE J J,DOYLE J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bull,1987,19(1):11-15.
[14]冯发强,张晶,王青峰,等.甜玉米LCYE等位基因多态性及功能分析[J].华南农业大学学报,2014,35(5):25-30.
[15]李晓旭,李家政.优化蒽酮比色法测定甜玉米中可溶性糖的含量[J].保鲜与加工,2013,4:24-27.
[16]中华人民共和国国家标准.谷物籽粒粗淀粉测定法:NY/T 11-1985[S].北京:中国标准出版社,1985.
[17]刘春泉,宋江峰,李大婧.鲜食甜糯玉米的营养及其加工[J].农产品加工,2010(6):8-9.
[18]RIBAUT J M,HOISINGTON D.Marker-assisted selection:New tools and strategies[J].Trends Plant Sci,1998,3(6):236-239.
[19]RIBAUT J M,JIANG C,HOISINGTON D.Simulation experiments on efficiencies of gene introgression by backcrossing[J].Crop Sci,2002,42(2):557-565.
[20]BABU R,NAIR S K,PRASANNA B M,et al.Integrating marker-assisted selection in crop breeding-Prospects and challenges[J].Curr Sci,2004,87(5):607-619.
[21]PRASANNA B M,PIXLEY K,WARBURTON M,et al.Molecular marker-assisted breeding options for maize improvement in Asia[J].Mol Breed,2010,26(2):339-356.
[22]FENG F,WANG Q,LIANG C,et al.Enhancement of tocopherols in sweet corn by marker-assisted backcrossing of ZmVTE4[J].Euphytica,2015,206(2):513-521.
[23]徐小万,雷建军,罗少波,等.作物基因聚合分子育种[J].植物遗传资源学报,2010,11(3):364-368.
[24]马军韬,张国民,辛爱华,等.水稻品种抗稻瘟病分析及基因聚合抗性改良[J].植物保护学报,2016,43(2):177-183.
[25]郝小琴,吴子恺.双隐性甜糯玉米的主要农艺及品质性状[J].作物学报,2003,29(3):321-329.
[26]郝小琴,吴子恺,张慧英.鲜食甜糯玉米子粒可溶性总糖含量的研究[J].玉米科学,2005,13(2):72-75.
[27]ZHANG S,HUANG Y,HE Z,et al.Variation law of pericarp tenderness of super sweet corn kernel[J].Agr Sci Technol,2016,17(7):1671-1674.
[2]刘蔚楠,万忠,甘阳英,等.2015年广东甜玉米产业发展形势与对策建议[J].广东农业科学,2016,43(3):12-16.
[3]李一男.国家玉米政策调整后鲜食玉米的发展机遇和方向[J].农业开发与装备,2017(9):60.
[4]祁显涛,李燕敏,谢传晓.玉米甜、糯性状育种的遗传学基础[J].玉米科学,2017,25(2):1-5.
[5]龙德祥,任晓菊,李勤,等.鲜食玉米育种概况及新品种选育方法[J].中国种业,2018(1):21-23.
[6]吴子恺.异隐纯合体杂交法与甜糯玉米育种[J].玉米科学,2003,11(3):13-17.
[7]张沛敏,邵林生,闫建宾,等.黑甜糯玉米新品种黑甜糯631的选育[J].中国蔬菜,2018(5):74-76.
[8]王萍,田凤芝,李晨.北方甜糯玉米优质高效栽培及发展前景[J].吉林蔬菜,2016(6):18-19.
[9]郝德荣,冒宇翔,陈国清,等.我国鲜食甜糯玉米育种现状与展望[J].浙江农业科学,2016,57(4):478-481.
[10]CREECH R G,MCARDLE F J.Gene interaction for quantitative changes in carbohydrates in maize kernels 1[J].Crop Sci,1966,6(2):192-194.
[11]李新海,白丽,彭泽斌,等.糯玉米育种技术研究进展[J].玉米科学,2003(专刊):14-16.
[12]杨耀迥,张述宽,滕辉升,等.应用分子标记辅助选择甜糯双隐基因型玉米种质[J].广西农业科学,2010,41(1):1-3.
[13]DOYLE J J,DOYLE J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bull,1987,19(1):11-15.
[14]冯发强,张晶,王青峰,等.甜玉米LCYE等位基因多态性及功能分析[J].华南农业大学学报,2014,35(5):25-30.
[15]李晓旭,李家政.优化蒽酮比色法测定甜玉米中可溶性糖的含量[J].保鲜与加工,2013,4:24-27.
[16]中华人民共和国国家标准.谷物籽粒粗淀粉测定法:NY/T 11-1985[S].北京:中国标准出版社,1985.
[17]刘春泉,宋江峰,李大婧.鲜食甜糯玉米的营养及其加工[J].农产品加工,2010(6):8-9.
[18]RIBAUT J M,HOISINGTON D.Marker-assisted selection:New tools and strategies[J].Trends Plant Sci,1998,3(6):236-239.
[19]RIBAUT J M,JIANG C,HOISINGTON D.Simulation experiments on efficiencies of gene introgression by backcrossing[J].Crop Sci,2002,42(2):557-565.
[20]BABU R,NAIR S K,PRASANNA B M,et al.Integrating marker-assisted selection in crop breeding-Prospects and challenges[J].Curr Sci,2004,87(5):607-619.
[21]PRASANNA B M,PIXLEY K,WARBURTON M,et al.Molecular marker-assisted breeding options for maize improvement in Asia[J].Mol Breed,2010,26(2):339-356.
[22]FENG F,WANG Q,LIANG C,et al.Enhancement of tocopherols in sweet corn by marker-assisted backcrossing of ZmVTE4[J].Euphytica,2015,206(2):513-521.
[23]徐小万,雷建军,罗少波,等.作物基因聚合分子育种[J].植物遗传资源学报,2010,11(3):364-368.
[24]马军韬,张国民,辛爱华,等.水稻品种抗稻瘟病分析及基因聚合抗性改良[J].植物保护学报,2016,43(2):177-183.
[25]郝小琴,吴子恺.双隐性甜糯玉米的主要农艺及品质性状[J].作物学报,2003,29(3):321-329.
[26]郝小琴,吴子恺,张慧英.鲜食甜糯玉米子粒可溶性总糖含量的研究[J].玉米科学,2005,13(2):72-75.
[27]ZHANG S,HUANG Y,HE Z,et al.Variation law of pericarp tenderness of super sweet corn kernel[J].Agr Sci Technol,2016,17(7):1671-1674.