扇贝壳堆积场中产蛋白酶放线菌的选育和鉴定
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摘要
为更快地处理崆峒岛废弃扇贝壳,改善岛屿的生态环境,本研究从扇贝堆积场取样,经过梯度稀释、平板涂布、平板划线、产酶菌初筛、复筛,分离筛选出蛋白酶产生菌20株,根据透明圈大小挑选出产蛋白酶活力强的8株放线菌菌株(SF1～SF8)进行斜面保藏。经过进一步理化鉴定初步确定SF1为诺卡氏菌,SF2～SF8属于链霉菌属。
To improve the ecological environment of Kongtong Island, twenty protease producing actinomycetic strains were isolated from scallop shells dumping ground on the Island. Eight strains which produce higher enzymatic activity(SF1 ～ SF8) were selected. After the further physical and chemical identification, SF1 was identified as Nocardia sp. and SF2 ～ SF8 were identified as Streptomyces spp.
To improve the ecological environment of Kongtong Island, twenty protease producing actinomycetic strains were isolated from scallop shells dumping ground on the Island. Eight strains which produce higher enzymatic activity(SF1 ～ SF8) were selected. After the further physical and chemical identification, SF1 was identified as Nocardia sp. and SF2 ～ SF8 were identified as Streptomyces spp.
引文
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[9]李树文,陆秀华,尚海利.碱性蛋白酶产生菌的筛选[J].莱阳农学院学报(自然版),2006,23(1):70-72.
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[11]胡学智.蛋白酶的生产和应用(下)[J].江苏调味副食品,2007,24(4):1-4.
[12]邵凤琴,韩庆祥.酶工程在污染治理中的应用[J].石油化工高等学校学报,2003,16(2):36-40.
[2]李筠,周宏霞,刘佳琳,等.青岛近岸特征环境中海洋异养细菌的分布规律及其分子鉴定[J].中国海洋大学学报,2006,36(6):965-970.
[3]韩文君,古静燕,李天东,等.一株产琼胶酶土壤细菌Paenibacillu ssp.MY03的筛选与分子鉴定[J].绵阳师范学院学报,2007,26(2):86-90.
[4]沈萍,范秀容,李广武,等.微生物学实验[M].北京:高等教育出版社,1999.
[5]Waksman S A.The Actinomycetes[M].Chronica Botanica Company,1950.
[6]张海涛,靳艳,吴佩春,等.一株海绵放线菌的分离鉴定及活性研究[J].微生物学通报,2004,31(5):60-64.
[7]张春枝,吕明宇,张润清,等.碱性纤维素酶生产菌的筛选及分类鉴定[J].大连轻工业学院院报,1994,13(1):51-58.
[8]陈启和,何国庆,应龙.弹性蛋白酶产生菌的筛选及其发酵条件的初步研究[J].浙江大学学报(农业与生命科学版),2003,29(1):59-64.
[9]李树文,陆秀华,尚海利.碱性蛋白酶产生菌的筛选[J].莱阳农学院学报(自然版),2006,23(1):70-72.
[10]Waksman S A,Leghrvaliler H A.Actinomycetes and their Antibiotigs[M].The Williams&Wilkins company,1958.
[11]胡学智.蛋白酶的生产和应用(下)[J].江苏调味副食品,2007,24(4):1-4.
[12]邵凤琴,韩庆祥.酶工程在污染治理中的应用[J].石油化工高等学校学报,2003,16(2):36-40.